Cloning of fatty acid biosynthetic genes β-ketoacyl CoA synthase, fatty acid elongase, stearoyl-ACP desaturase, and fatty acid desaturase and analysis of expression in the early developmental stages of flax (Linum usitatissimum L.) seeds

Plant Science

Volumn 166, Issue 6, 2004, Pages 1487-1496

  Fofana, Bourlaye ^a   Duguid, Scott ^b   Cloutier, Sylvie ^a  

^a Cereal Research Centre   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

cDNA library; Condensing enzymes; Desaturases; Fatty acid biosynthesis; Omega 3; Omega 6 fatty acids

Indexed keywords

BIOSYNTHESIS; DNA; ENZYMES; FATTY ACIDS; FOOD PRODUCTS; GENES;

BIOSYNTHETIC PATHWAYS; CONDENSING PROTEINS; FLAXSEED PRODUCTS; MULTIPLE SEQUENCE ALIGNMENT;

CLONING;

LINUM; LINUM USITATISSIMUM;

EID: 1942478427     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plantsci.2004.01.025     Document Type: Article

References (47)

1. Hasler C.M., Kundrat S., Wool D. Functional foods and cardiovascular disease. Curr. Arteroscler. Rep. 2:2000;467-475.
2. Watkins B.A., Devitt A.A., Feng S. Designed eggs containing conjugated linoleic acids and omega-3 polyunsaturated fatty acids. World Rev. Nutr. Diet. 90:2001;162-182.
3. Lemay A., Dodin S., Kadri N., Jacques H., Forest J.C. Flaxseed dietary supplement versus hormone replacement therapy in hypercholesterollemic menopausal women. Obstet. Gynecol. 100:2002;495-504.
4. Ogborn M.R., Nitschmann E., Bankovic-Calic N., Weiler H.A., Aukema H. Dietary flax oil reduces renal injury, oxidized LDL content, and tissue n-6/n-3 FA ration in experimental polycystic kidney disease. Lipids. 37:2002;1059-1065.
5. Cunnane S.C., Ganguli S., Menard C., Liede A.C., Hamadeh M.J., Chen Z.Y., Wolever T.M., Jenkins D.J. High alpha-linolenic acid flaxseed (Linum usitatissimum): some nutritional properties in humans. Br. J. Nutr. 69:1993;443-453.
6. O'Neill W., McKee S., Clarke A.F. Flaxseed (Linum usitatissimum) supplementation associated with reduced skin test lesional area in horses with Culicoides hypersensitivity. Can. J. Vet. Res. 66:2002;272-277.
7. Simopoulos A.P. Essential fatty acids in health and chronic disease. Am. J. Clin. Nutr. 70:1999;560S-569S.
8. Simopoulos A.P. The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed. Pharmacother. 56:2002;365-379.
9. Weber P., Raederstorff D. Triglyceride-lowering effect of omega-3 LC-polyunsaturated fatty acids - a review. Nutr. Metab. Cardiovasc. Dis. 10:2000;28-37.
10. Voelker T., Kinney A.J. Variation in the biosynthesis of seed-storage lipids. Ann. Rev. Plant Physiol. Plant Mol. Biol. 52:2001;335-361.
11. Thelen J.J., Ohlrogge J.B. Metabolic engineering of fatty acid biosynthesis in plants. Metabol. Eng. 4:2002;12-21.
12. Wallis J.G., Watts J.L., Browse J. Polyunsaturated fatty acid synthesis: what will they think of next. TIBS. 27:2002;467-473.
13. Tai H., Jaworski J.G. 3-Ketoacyl-acyl carrier protein synthase III from spinach (Spinacha oleracea) is not similar to other condensing enzymes of fatty acid synthase. Plant Physiol. 103:1993;1361-1367.
14. Shimakata T., Stumpf P.K. Isolation and function of spinach leaf β-ketoacyl-[acyl-carrier-protein] synthase. Proc. Natl. Acad. Sci. U. S. A. 79:1982;5808-5812.
15. Kauppinen S., Siggaard-Anderson M., von Wettstein-Knowles P. β-Ketoacyl-ACP synthase I of Echerichia coli nucleotide sequence of the fabB gene and identification of the cerulenin binding residue. Carlsberg Res. Commun. 53:1988;357-370.
16. Dehesh K., Edwards P., Fillatti J., Slabaugh M., Byrne J. KAS IV: a 3-ketoacyl-ACP synthase from Cuphea sp. is a medium chain specific condensing enzyme. Plant J. 15:1998;383-390.
17. Dehesh K., Tai H., Edwards P., Byrne J., Jaworski J.G. Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis. Plant Physiol. 125:2001;1103-1114.
18. Shanklin J., Somerville C. Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. Proc. Natl. Acad. Sci. U. S. A. 88:1991;2510-2514.
19. Knutzon D.S., Thompson G.A., Radke S.E., Johnson W.B., Knauf V.C., Kridl J.C. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc. Natl. Acad. Sci. U. S. A. 89:1992;2624-2628.
20. Mikkilineni V., Rocheford T.R. Sequence variation and genomic organization of fatty acid desaturase-2 (FAD2) and fatty acid desaturase-6 (FAD6) cDNAs in maize. Theor. Appl. Genet. 106(1):2003;326-1332.
21. Iba K., Gibson S., Nishiuchi T., Fuse T., Nishimura M., Aronde V., Hugly S., Somerville C. A gene encoding a chloroplast ω-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the FAD7 mutant of Arabidopsis thalina. J. Biol. Chem. 268:1993;24099-24105.
22. Miquel M.F., Browse J.A. High-oleate oilseeds fail to develop at low temperature. Plant Physiol. 106:1994;421-427.
23. Schultz D.J., Suh M.C., Ohlrogge J.B. Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influenced by ferredoxin. Plant Physiol. 124:2000;681-692.
24. Han J., Luhs W., Sonntag K., Zahringer U., Borchardt D.S., Wolter F.P., Heinz E., Frentzen M. Functional characterization of beta-ketoacyl-CoA synthase genes from Brassica napus L. Plant Mol. Biol. 46:2001;229-239.
25. El-Shattory Y. Chromatographic column fractionation and fatty acid composition of different lipid classes of linseed oil. Nahrung. 20:1976;307-311.
26. Slack C.R., Roughan P.G. Rapid temperature-induced changes in the fatty acid composition of certain lipids in developing linseed and soya-bean cotyledons. Biochem. J. 170:1978;437-439.
27. Bhatia I.S., Sukhija P.S. Changes in fatty acids of linseed oil (Linum usitatissimum) during ripening. Indian J. Biochem. 7:1970;215-216.
28. Slack C.R., Bertaud W.S., Shaw B.D., Holland R., Browse J., Wright H. Some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower (Carthamus tinctorius) and linseed (Linum ustatissimum). Biochemistry. 190:1980;551-561.
29. Wanasundara J.P.D., Shahidi F. Functional properties and amino-acid composition of solvent-extracted flaxseed meals. Food Chem. 49:1994;45-51.
30. Oomah D.B., Kenaschuk E.O., Mazza G. Phytic acid content of flaxseed as influenced by cultivar, growing season, and location. J. Agric. Food Chem. 44:1996;2663-2666.
31. Singh S., McKinney S., Green A. Sequence of a cDNA from Linum usitatissimum encoding the stearoyl-ACP carrier protein desaturase. Plant Physiol. 140:1994;1075.
32. Altschul S.F., Madden T.L., Shaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:1997;3389-3402.
33. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:1994;4673-4680.
34. O'Hara P., Slabas A.R., Fawcett T. Fatty acid and lipid biosynthetic genes are expressed at constant molar ratios but different absolute levels during embryogenesis. Plant Physiol. 129:2002;310-320.
35. Moon H., Smith M.A., Kunst L. A condensing enzyme from the seeds of Lesquerella fendleri that specifically elongates hydroxyl fatty acids. Plant Physiol. 127:2001;1635-1643.
36. Millar A.A., Kunst L. Very-long-chain fatty acid biosynthesisis is controlled through the expression and specificity of the condensing enzyme. Plant J. 12:1997;121-131.
37. Ghanevati M., Jaworski J.G. Engineering and mechanistic studies of the Arabidopsis FAE1-ketoacyl-CoA synthase, FAE1 KCS. Eur. J. Biochem. 269:2002;3531-3539.
38. Emanuelsson O., Nielsen H., Brunak S., von Heijne C. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300:2000;1005-1016.
39. Shanklin J., Cahoon E.B. Desaturation and related modifications of fatty acids. Ann. Rev. Plant Physiol. Plant Mol. Biol. 49:1998;611-641.
40. Jin U.-H., Lee J.-W., Chung Y.-S., Lee J.-H., Yi Y.B., Kim Y.-K., Hyung N.-I., Pyee J.-H., Chung C.-H. Characterization and temporal expression of a ω-6 fatty acid desaturase cDNA from sesame (Sesamum indicum L.) seeds. Plant Sci. 161:2001;935-941.
41. Domergue F., Spiekermann P., Lerchl J., Beckmann C., Kilian O., Kroth P.G., Boland W., Zahringer U., Heinz E. New insight into Phaeodactylum tricornutum fatty acid metabolism, Cloning and functional characterization of plastidial and microsomal Δ12-fatty acid desaturase. Plant Physiol. 131:2003;1648-1660.
42. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 44:1987;406-425.
43. Heppard E.P., Kinney A.J., Stecca K.L., Miao G.H. Developmental and growth temperature regulation of two different microsomal omega-6 desaturase genes in soybeans. Plant Physiol. 110:1996;311-319.
44. Liu Q., Singh S.P., Brubaker C.L., Sharp P.J., Green A.G., Marshall D.R. Molecular cloning and expression of cDNA encoding a microsomal ω-6 fatty acid desaturase from cotton (Gossypium hirsutum). Aust. J. Plant Physiol. 26:1999;101-106.
45. Verwoert I., Meller-Harel Y., van der Linden K., Verbree B., Koes R., Stuitje A. The molecular basis of the high linoleic acid content in Petunia seed oil: analysis of a seed-specific linoleic acid mutant. Biochem. Soc. Trans. 28:2000;631-632.
46. Ishiguro S., Kawai-Oda A., Ueda J., Nishida I., Okada K. The defective in anther dehiscence1 gene encodes a novel phospholipase A1 catalysing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis. Plant Cell. 13:2001;2191-2209.
47. Suzuki M., Yamaguchi S., Iida T., Hashimoto I., Teranishi H., Mizoguchi M., Yano F., Todoroki Y., Watanabe N., Yokoyama1 M. Endogenous-ketol linolenic acid levels in short day-induced cotyledons are closely related to flower induction in Pharbitis nil. Plant Cell Physiol. 44:2003;135-143.