Expression of functional β2-adrenergic receptors in the lung epithelial cell lines 16HBE14o-, Calu-3 and A549

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1691, Issue 2-3, 2004, Pages 169-179

  Abraham, Getu ^a   Kneuer, Carsten ^a   Ehrhardt, Carsten ^b   Honscha, Walther ^a   Ungemach, Fritz R ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

2 Adrenoceptor; 2 AR mRNA; Cell culture; Cyclic AMP; Lung epithelial cells

Indexed keywords

3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; 5 [2 [[2 HYDROXY 3 [4 (1 METHYL 4 TRIFLUOROMETHYL 2 IMIDAZOLYL)PHENOXY]PROPYL]AMINO]ETHOXY]SALICYLAMIDE; ADENYLATE CYCLASE; BETA 1 ADRENERGIC RECEPTOR BLOCKING AGENT; BETA 2 ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR BLOCKING AGENT; CYANOPINDOLOL I 125; CYCLIC AMP; ISOBUTYLMETHYLXANTHINE; ISOPRENALINE; MESSENGER RNA; RADIOLIGAND; RECEPTOR SUBTYPE; TRITIUM; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CYANOIODOPINDOLOL; ISOPROTEIN;

ADRENERGIC SYSTEM; ARTICLE; BINDING ASSAY; CELL MEMBRANE; CELL STIMULATION; CELL STRAIN CACO 2; CELL STRAIN COS1; CELL SURFACE; CELL TYPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; DISSOCIATION CONSTANT; EPITHELIUM CELL; EXPERIMENTAL MODEL; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOCYTOCHEMISTRY; LIGAND BINDING; LUNG ALVEOLUS EPITHELIUM; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; RADIOASSAY; RADIOIMMUNOASSAY; RECEPTOR DENSITY; REGULATORY MECHANISM; RESPIRATORY EPITHELIUM; RESPIRATORY SYSTEM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SYNTHESIS; ANIMAL; CELL LINE; CYTOLOGY; DOSE RESPONSE; GENETICS; LUNG; METABOLISM;

MAMMALIA;

ADENYLATE CYCLASE; ADRENERGIC BETA-AGONISTS; ADRENERGIC BETA-ANTAGONISTS; ANIMALS; CELL LINE; CYCLIC AMP; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIAL CELLS; HUMANS; IODOCYANOPINDOLOL; ISOPROTERENOL; LUNG; PROTEIN ISOFORMS; RADIOLIGAND ASSAY; RECEPTORS, ADRENERGIC, BETA-2;

EID: 1942444543     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamcr.2004.02.002     Document Type: Article

References (44)

1. Barnes P.J. Novel approaches and targets for treatment of chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 160:1999;S72-S79.
2. Barnes P.J. Effect of beta-agonists on inflammatory cells. J. Allergy Clin. Immunol. 104:1999;10-17.
3. Barnes P.J. Beta-adrenergic receptors and their regulation. Am. J. Respir. Crit. Care Med. 152:1995;838-860.
4. Salathe M. Effects of beta-agonists on airway epithelial cells. J. Allergy Clin. Immunol. 110:2002;275-281. (Suppl).
5. Nijkamp F.P., Engels F., Henricks P.A., Van Oosterhout A.J. Mechanisms of beta-adrenergic receptor regulation in lungs and its implications for physiological responses. Physiol. Rev. 72:1992;323-367.
6. Van der Velden V.H.J., Savelkaoul H.F.J., Versnel M.A. Bronchial epithelium: morphology, function and pathophysiology of asthma. Eur. Cytokine Netw. 9:1998;585-597.
7. Polito A.J., Proud D. Epithelia cells as regulators of airway inflammation. J. Allergy Clin. Immunol. 102:1998;714-718.
8. Folkerts G., Nijkamp F.P. Airway epithelium: more than just a barrier. Trends Pharmacol. Sci. 19:1998;334-341.
9. Aksoy M.O., Mardini I.A., Yang Y., Bin W., Zhou S., Kelsen S.G. Glucocorticoid effects on the beta-adrenergic receptor-adenylyl cyclase system of human airway epithelium. J. Allergy Clin. Immunol. 109:2002;491-497.
10. Seldon P.M., Barnes P.J., Meja K., Giembycz M.A. Suppression of lipopolysaccharide-induced tumor necrosis factor-alpha generation from human peripheral blood monocytes by inhibitors of phosphodiesterase 4: interaction with stimulants of adenylyl cyclase. Mol. Pharmacol. 48:1995;747-757.
11. Izeboud C.A., Vermeulen R.M., Zwart A., Voss H.P., van Miert A.S., Witkamp R.F. Stereoselectivity at the beta2-adrenoceptor on macrophages is a major determinant of the anti-inflammatory effects of beta2-agonists. Naunyn-Schmiedeberg's Arch. Pharmacol. 362:2000;184-189.
12. Turki J., Green S.A., Newman K.B., Meyers M.A., Liggett S.B. Human lung cell beta 2-adrenergic receptors desensitize in response to in vivo administered beta-agonist. Am. J. Physiol. 269:1995;L709-L714.
13. McGraw D.W., Forbes S.L., Mak J.C., Witte D.P., Carrigan P.E., Leikauf G.D., Liggett S.B. Transgenic overexpression of beta(2)-adrenergic receptors in airway epithelial cells decreases bronchoconstriction. Am. J. Physiol. 279:2000;L379-L389.
14. Kelsen S.G., Anakwe O.O., Aksoy M.O., Reddy P.J., Dhanesekaran N., Penn R., Benovic J.L. Chronic effects of catecholamines on the beta 2-adrenoreceptor system in cultured human airway epithelial cells. Am. J. Physiol. 272:1997;L916-L924.
15. Mizuno Y., Ito Y., Aoyama M., Kume H., Nakayama S., Yamaki K. Imipramine inhibits Cl(-) secretion by desensitization of beta-adrenergic receptors in calu-3 human airway cells. Biochem. Biophys. Res. Commun. 274:2000;620-625.
16. Zhang Y., Reenstra W.W., Chidekel A. Antibacterial activity of apical surface fluid from the human airway cell line calu-3: pharmacologic alteration by corticosteroids and b2-agonists. Am. J. Respir. Cell Mol. Biol. 25:2001;196-202.
17. Nishimura K., Tamaoki J., Isono K., Aoshiba K., Nagai A. Beta-adrenergic receptor-mediated growth of human airway epithelial cell lines. Eur. Respir. J. 20:2002;353-358.
18. Abraham G., Schusser G.F., Ungemach F.R. Dexamethasone-induced increase in lymphocyte beta-adrenergic receptor density and cAMP formation in vivo. Pharmacology. 67:2003;1-5.
19. 125I]-iodocyanopindolol. Equine Vet. J. 33:2001;487-493.
20. Scatchard G. The interaction of proteins for molecules and ions. Ann. N.Y. Acad. Sci. 51:1949;660-672.
21. Cheng Y., Prusoff W.H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 percent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22:1973;3099-3108.
22. Bilski A.J., Halliday S.E., Fitzgerald J.D., Wale J.L. The pharmacology of a beta 2-selective adrenoceptor antagonist (ICI 118,551). J. Cardiovasc. Pharmacol. 5:1983;430-437.
23. Dooley D.J., Bittiger H., Reymann N.C. CGP 20712 A: a useful tool for quantitating beta 1- and beta 2-adrenoceptors. Eur. J. Pharmacol. 130:1986;137-139.
24. Carstairs J.R., Nimmo A.J., Barnes P.J. Autoradiographic visualization of beta-adrenoceptor subtypes in human lung. Am. Rev. Respir. Dis. 132:1985;541-547.
25. Davis P.B., Silski C.L., Kercsmar C.M., Infeld M. Beta-adrenergic receptors on human tracheal epithelial cells in primary culture. Am. J. Physiol. 258:1990;C71-C76.
26. Amstad P., Reddel R.R., Pfeifer A., Malan-Shibley L., Mark G.E., Harris C.C. Neoplastic transformation of a human bronchial epithelial cell line by a recombinant retrovirus encoding viral Harvey ras. Mol. Carcinog. 1:1988;151-160.
27. Shen B.Q., Finkbeiner W.E., Wine J.J., Mrsny R.J., Widdicombe J.H. Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl-secretion. Am. J. Physiol. 266:1994;L493-L501.
28. Cozens A.L., Yezzi M.J., Kunzelmann K., Ohrui T., Chin L., Eng K., Finkbeiner W.E., Widdicombe J.H., Gruenert D.C. CFTR expression and chloride secretion in polarized immortal human bronchialepithelial cells. Am. J. Respir. Cell Mol. Biol. 10:1994;38-47.
29. Dorscheid D.R., Conforti A.E., Hamann K.J., Rabe K.F., White S.R. Characterization of cell surface lectin-binding patterns of human airway epithelium. Histochem. J. 31:1999;145-151.
30. -: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies. Cell Tissue Res. 308:2002;391-400.
31. Kunzelmann K., Koslowsky T., Hug T., Gruenert D.C., Greger R. cAMP-dependent activation of ion conductances in bronchial epithelial cells. Pflügers Arch. 428:1994;590-596.
32. Linden A. Increased interleukin-8 release by b-adrenoceptor activation in human transformed bronchial epithelial cells. Br. J. Pharmacol. 119:1996;402-406.
33. Elbert K.J., Schafer U.F., Schafers H.J., Kim K.J., Lee V.H., Lehr C.M. Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies. Pharm. Res. 16:1999;601-608.
34. Szentendrei T., Lazar-Wesley E., Nakane T., Virmani M., Kunos G. Selective regulation of beta2-adrenergic receptor gene expression by interleukin-1 in cultured human lung tumor cells. J. Cell. Physiol. 152:1992;478-485.
35. Factor P., Adir Y., Mutlu G.M., Burhop J., Dumasius V. Effects of beta2-adrenergic receptor overexpression on alveolar epithelial active transport. J. Allergy Clin. Immunol. 110:2002;S242-S246.
36. Henry P.J., Rigby P.J., Goldie R.G. Distribution of beta 1- and beta 2-adrenoceptors in mouse trachea and lung: a quantitative autoradiographic study. Br. J. Pharmacol. 99:1990;136-144.
37. Agrawal D.K., Schugel J.W., Townley R.G. Comparison of beta adrenoceptors in bovine airway epithelium and smooth muscle cells. Biochem. Biophys. Res. Commun. 148:1987;178-183.
38. Kelsen S.G., Higgins N.C., Zhou S., Mardini I.A., Benovic J.L. Expression and function of the beta-adrenergic receptor coupled-adenylyl cyclase system on human airway epithelial cells. Am. J. Respir. Crit. Care Med. 152:1995;1774-1783.
39. Penn R.B., Kelsen S.G., Benovic J.L. Regulation of beta-agonist- and prostaglandin E2-mediated adenylyl cyclase activity in human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 11:1994;496-505.
40. Nogami M., Romberger D.J., Rennard S.I., Toews M.L. Agonist-induced desensitization of beta-adrenoceptors of bovine bronchial epithelial cells. Clin. Sci. (Lond.). 85:1993;651-657.
41. Penn R.B., Shaver J.R., Zangrilli J.G., Pollice M., Fish J.E., Peters S.P., Benovic J.L. Effects of inflammation and acute beta-agonist inhalation on beta 2-AR signaling in human airways. Am. J. Physiol. 271:1996;L601-L608.
42. del Monte F., Kaumann A.J., Poole-Wilson P.A., Wynne D.G., Pepper J., Harding S.E. Coexistence of functioning beta 1- and beta 2-adrenoceptors in single myocytes from human ventricle. Circulation. 88:1993;854-863.
43. Milligan G., Bond R.A. Inverse agonism and the regulation of receptor number. Trends Pharmacol. Sci. 18:1997;468-474.
44. Strossberg A.D. Structure and function of the beta 3-adrenergic receptor. Annu. Rev. Pharmacol. Toxicol. 37:1997;421-450.