Rapid screening for HLA-B27 by a TaqMan-PCR assay using sequence-specific primers and a minor groove binder probe, a novel type of TaqMan™ probe

Journal of Immunological Methods

Volumn 287, Issue 1-2, 2004, Pages 179-186

  Sylvain, Kuzio ^a   Aurélie, Hanguehard ^b   Marc, Morelle ^b   Christophe, Ronsin ^b  

^a Lab d'Analyses Medicales Ruffie A   (France)

^b LCL   (France)

Author keywords

Rn; Ct; HLA B27; Melting temperature; MGB; MGB probe; Minor groove binder; Minor groove binder probe; MLCT; Normalized reporter signal; PCR SSP; Real time PCR; Sequence specific primers; SSP; Tm; TaqMan; Threshold cycle

Indexed keywords

HLA B27 ANTIGEN;

ANALYTIC METHOD; ANTIGEN DETECTION; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; GENE SEQUENCE; HUMAN; MELTING POINT; MOLECULAR PROBE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SCREENING TEST; SEROLOGY; VALIDATION PROCESS;

DNA PRIMERS; FLOW CYTOMETRY; GENETIC PREDISPOSITION TO DISEASE; GENOTYPE; HLA-B27 ANTIGEN; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RISK FACTORS; SENSITIVITY AND SPECIFICITY; SPONDYLITIS, ANKYLOSING; TAQ POLYMERASE;

EID: 1942424202     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.01.027     Document Type: Article

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