Determining PPARγ-ligand binding affinity using fluorescent assay with cis-parinaric acid as a probe

Science in China, Series B: Chemistry

Volumn 48, Issue 2, 2005, Pages 122-131

  Gao, Zhenting ^a   Luo, Haibin ^a   Chen, Lili ^a   Shen, Jianhua ^a   Chen, Kaixian ^a   Jiang, Hualiang ^a   Shen, Xu ^a  

^a SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

cis parinaric acid; Displacement assay; Equilibrium dissociation constant; Fluorescent probe; IC50; PPAR

Indexed keywords

AFFINITY CHROMATOGRAPHY; CURVE FITTING; DISSOCIATION; PROBES; PROTEINS;

CIS PARINARIC ACID; DISPLACEMENT ASSAY; EQUILIBRIUM DISSOCIATION CONSTANT; FLUORESCENT PROBE; LIGAND BINDING AFFINITY; RECEPTOR;

BINDING ENERGY;

EID: 19044377748     PISSN: 10069291     EISSN: None     Source Type: Journal    
DOI: 10.1360/03yb0217     Document Type: Article

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