Purification and partial characterisation of an acid lipase in germinating lipidbody linseedlings

Turkish Journal of Botany

Volumn 29, Issue 3, 2005, Pages 177-184

  Sammour, Reda H ^a  

^a Tanta University   (Egypt)

Author keywords

Acid lipase; Ether extracted lipid body membrane protein (ELBP); Triton X 100 solubilised lipid body membrane protein (XLBP)

Indexed keywords

ENZYME;

EID: 18944401719     PISSN: 1300008X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Beisson F, Ferté N, Nari J, Noat G, Arondel, V & Verger R (1999). Use of naturally fluorescent triacylglycerols from Parinari glaberrimum to detect low lipase activities from Arabidopsis thaliana seedlings. Journal of Lipid Research 40: 1033-1040.
2. Beisson F, Arondel V & Verger R (2000). Assaying Arabidopsis lipase activity. Biochem Soc Trans 28: 773-775.
3. Ben Miled DD, Zarrouk M & Cherif A (2000). Sodium chloride effects on lipase activity in germinating rape seeds. Biochem Soc Trans 28: 899-902.
4. Bhardwaj K, Raju A & Rajasekharan R (2001). Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (phospho) lipase family. Plant Physiol 127: 1728-1738.
5. Bradford MM (1976). A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
6. Edqvist J & Farbos I (2002). Characterization of germination-specific lipid transfer proteins from Euphorbia lagascae. Planta 215: 41-50.
7. Fischer M & Pleiss J (2003). "The Lipase Engineering Database: a navigation and analysis tool for protein families". Nucl Acid Res 31: 319-321.
8. Folch J, Less M & Stainley GHS (1957). A simple method for the isolation and purification of total lipids from animal tissues. Biol Chem J 226 : 497-509.
9. Gandhi NN & Mukherjee KD (2000). Specificity of papaya lipase in esterification with respect to the chemical structure of substrates. J Agric Food Chem 48: 566-70.
10. Hammer MF & Murphy JB (1993). Properties of the lipid body lipase of Pinus edulis and electrophoretic purification of its 64 kDa protein. Physiologia Plantarum 87: 39-44.
11. Hammer MF & Murphy JB (1994). Lipase activity and in vivo triacylglycerol utilization during Pinus edulis seed germination. Plant Physiol Biochem 32: 861.
12. Hills MJ & Murphy DJ (1988). Characterization of lipases from the lipid bodies and microsomal membranes of erucic acid-free ouseed-rape (Brassica napus) cotyledons. Biol Chem J 249: 687-693.
13. Huang AHC (1985). Lipid bodies. 1. In: Linkins HF & Jackson F (eds). Modern Methods of Plant Analysis. Berlin: Springer Verlag. 145-151.
14. Huang AHC, Qu R, Lal YK, Ratnayake C, Chan KL, Kurok GW, Oo KC & Cao YZ (1991). Structure, synthesis and degradation of oil bodies in maize. In: Emes M (ed). Compartmentation of Plant Metabolism in Non-Photòsynthesis Tissues. Cambridge: Cambridge University Press. 43-58.
15. Huang AHC (1996). Oleosins and oil bodies in seeds and other organs. Plant Physiol 110: 1055-1061
16. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
17. Lin YH & Huang AH (1983). Lipase in lipid bodies of cotyledons of rape and mustard seedlings. Arch Biochem Biophys 225: 360-369.
18. Lin YH & Huang AHC (1984). Purification and initial characterization of lipase from the scutella of corn seedling. Plant Physiol 76: 719-722.
19. Lin YH, Yu C & Huang AHC (1986). Substrate specificities of lipases from corn and other seeds. Arch. Biochem Biophys 244: 346-356.
20. Luddy FE, Barford RA, Herb SF & Paul M (1968). A rapid quantitative procedure for the preparation of methyl esters of butter fat and other fats. J Am Oil Chem Soc 45: 549-552.
21. Maeshima M & Beevers H (1985). Purification and properties of glyoxysomal lipase from castor bean. Plant Physiol 79: 489-493.
22. Miled N, Canaan S, Dupuis L, Roussel A, Riviere M, Carriere F, de Caro A, Cambillau C & Verger R (2000). Digestive lipases: from three-dimensional structure to physiology. Biochimie 82: 973-86.
23. Millichip M, Tatham AS, Jackson F, Griffiths G, Shewry PR & Stobart AK (1996). Purification and characterization of oil-bodies (oleosomes) and oil-body boundary proteins (oleosins) from the developing cotyledons of sunflower (Helianthus annuus L.). Biochem J 314: 333-337.
24. Moulin A, Teissere M, Bernard C & Pieroni G (1994). Lipases of the Euphorbiaceae family: purification of a lipase from Euphorbia characias latex and structure - function relationships with the B chain of ricin. Proc Natl Acad Sci 91: 11328-11332.
25. Murphy DJ & Cummins I (1990). Seed oil-bodies: Isolation, composition and role of oil-body apolipoproteins. Phytochemistry 28: 2063-2069.
26. Muto, S & Beevers, H (1974). Lipase activities in castor bean endosperm during germination. Plant Physiol 54: 23-28.
27. Napier JA, Stobart AK & Shewry PR (1996). The structure and biogenesis of plant oil bodies: The role of the ER membrane and the oleosin class of proteins. Plant Mol Biol 31: 945.
28. Ncube I, Gitlesen T, Adlercreutz P, Read JS & Mattiassson B (1995) Fatty acid selectivity of a lipase purified from Vernonia galamensis seed. Biochim Biophys Acta 1257: 149-156.
29. Ory RL, Angelo AJ St. & Altschul AM (1962). The acid lipase of the castor bean. Properties and substrate specificity. J Lipid Research 3: 99-105.
30. Ory RL (1969). Acid lipase of the castor bean. Lipids 4: 177-185.
31. Roussel A, Miled N, Berti-Dupuis L, Riviere M, Spinelli S, Berna P, Gruber V, Verger R & Cambillau C (2002). Crystal structure of the open form of dog gastric lipase in complex with a phosphonate inhibitor. J Biol Chem 277: 2266-2274.
32. Sammour RH, El-Shourbagy MN, Abo-Shady AM & Abasery AM (1994). The seed proteins of linseed (Linum usitatissimum L.). Bot Bull Acad Sin 38: 171-177.
33. Shimizu S & Nakano M (2003). Structural characterization of triacylglycerol in several oils containing gamma-linolenic acid. Biosci Biotechnol Biochem 67: 60-7.
34. Siedow JN (1991). Plant lipoxygenase: structure and function. Annu Rev Plant Physiol 42: 145-188.
35. Stymme S & Stobart AK (1987). Triacyl glycerol biosynthesis, 10. In: Stumpf PK & Coan EE (ed.). The Biochemistry of Plants. New York: Academic Press. 175-214.
36. Towbin H, Staehelin T & Gorden J (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedures and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
37. Tsydendambaev VD & Vereshchagin (2003). Changes in triacylglycerol composition during ripening of sea buckthorn (Hippophae rhamnoides L.) seeds. J Agric Food Chem 51: 1278-1283.
38. Tzen TC, Cao YZ, Laurent P, Ratnayake C & Huang AHC (1993). Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiol 101: 267-276.
39. Stymme S & Stobart AK (1987). Triacyl glycerol biosynthesis, 10. In: Stumpf PK & Coan EE (eds). The Biochemistry of plants. New York: Academic Press. 175-214.
40. Yatsu LY & Jacks TL (1972). Spherosome membranes half unit-membranes. Plant Physiol 49: 937-943.