UV-induced DNA damage in human keratinocytes: Quantitation and correlation with long-term survival

Experimental Dermatology

Volumn 14, Issue 5, 2005, Pages 349-355

  Lisby, Steen ^a   Gniadecki, Robert ^a   Wulf, Hans Christian ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

D37; DNA damage; Keratinocytes; Ultraviolet radiation; XL PCR

Indexed keywords

ARTICLE; CARCINOGENESIS; CELL CULTURE; CELL CYCLE; CELL DEATH; CELL GROWTH; CELL STRAIN; CELL SURVIVAL; CLONOGENESIS; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DAMAGE; GENE EXPRESSION; GROWTH INHIBITION; HUMAN; HUMAN CELL; KERATINOCYTE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RADIATION DOSE; RADIATION DOSE DISTRIBUTION; RADIATION INJURY; SKIN CANCER; ULTRAVIOLET A RADIATION; ULTRAVIOLET B RADIATION;

CELL LINE, TRANSFORMED; CELL SURVIVAL; CYTOLOGICAL TECHNIQUES; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, RADIATION; GLOBINS; HUMANS; KERATINOCYTES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; ULTRAVIOLET RAYS;

EID: 18844374818     PISSN: 09066705     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0906-6705.2005.00282.x     Document Type: Article

References (26)

1. Ravanat J L, Douki T, Cadet J. Direct and indirect effects of UV radiation on DNA and its components. J Photochem Photobiol B 2001: 63: 88-102.
2. Rosenstein B S, Mitchell D L. Action spectra for the induction of pyrimidine(6-4)pyrimidone photoproducts and cyclobutane pyrimidine dimers in normal human skin fibroblasts. Photochem Photobiol 1987: 45: 775-780.
3. Wikonkal N M, Brash D E. Ultraviolet radiation induced signature mutations in photocarcinogenesis. J Investig Dermatol Symp Proc 1999: 4: 6-10.
4. Lai L W, Ducore J M, Rosenstein B S. DNA-protein crosslinking in normal human skin fibroblasts exposed to solar ultraviolet wavelengths. Photochem Photobiol 1987: 46: 143-146.
5. Drouin R, Therrien J P. UVB-induced cyclobutane pyrimidine dimer frequency correlates with skin cancer mutational hotspots in p53. Photochem Photobiol 1997: 66: 719-726.
6. Bohr V A, Smith C A, Okumoto D S, Hanawalt P C. DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall. Cell 1985: 40: 359-369.
7. Laws G M, Skopek T R, Reddy M V, Storer R D, Glaab W E. Detection of DNA adducts using a quantitative long PCR technique and the fluorogenic 5′ nuclease assay (TaqMan). Mutat Res 2001: 484: 3-18.
8. Yarosh D B, Boumakis S, Brown A B et al. Measurement of UVB-induced DNA damage and its consequences in models of immunosuppression. Methods 2002: 28: 55-62.
9. Sutherland J C, Monteleone D C, Trunk J G, Bennett P V, Sutherland B M. Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis. Electrophoresis 2001: 22: 843-854.
10. Fairbairn D W, Olive P L, O'Neill K L. The comet assay: a comprehensive review. Mutat Res 1995: 339: 37-59.
11. Dizdaroglu M. The use of capillary gas chromatography-mass spectrometry for identification of radiation-induced DNA base damage and DNA base-amino acid cross-links. J Chromatogr 1984: 295: 103-121.
12. Douki T, Cadet J. Individual determination of the yield of the main UV-induced dimeric pyrimidine photoproducts in DNA suggests a high mutagenicity of CC photolesions. Biochemistry 2001: 40: 2495-2501.
13. Ravanat J L, Turesky R J, Gremaud E, Trudel L J, Stadler R H. Determination of 8-oxoguanine in DNA by gas chromatography - mass spectrometry and HPLC - electrochemical detection: overestimation of the background level of the oxidized base by the gas chromatography - mass spectrometry assay. Chem Res Toxicol 1995: 8: 1039-1045.
14. Cadet J, Douki T, Ravanat J L. Artifacts associated with the measurement of oxidized DNA bases. Environ Health Perspect 1997: 105: 1034-1039.
15. Petersen A B, Gniadecki R, Vicanova J, Thorn T, Wulf H C. Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes. J Photochem Photobiol B 2000: 59: 123-131.
16. Vollmer A C, Belkin S, Smulski D R, Van Dyk T K, LaRossa R A. Detection of DNA damage by use of Escherichia coli carrying recA′::lux, uvrA′::lux, or alkA′::lux reporter plasmids. Appl Environ Microbiol 1997: 63: 2566-2571.
17. White C I, Sedgwick S G. The use of plasmid DNA to probe DNA repair functions in the yeast Saccharomyces cerevisiae. Mol Gen Genet 1985: 201: 99-106.
18. Kalinowski D P, Illenye S, Van Houten B. Analysis of DNA damage and repair in murine leukemia L1210 cells using a quantitative polymerase chain reaction assay. Nucleic Acids Res 1992: 20: 3485-3494.
19. Chandrasekhar D, Van Houten B. High resolution mapping of UV-induced photoproducts in the Escherichia coli lacI gene. Inefficient repair of the non-transcribed strand correlates with high mutation frequency. J Mol Biol 1994: 238: 319-332.
20. Van Houten B, Cheng S, Chen Y. Measuring gene-specific nucleotide excision repair in human cells using quantitative amplification of long targets from nanogram quantities of DNA. Mutat Res 2000: 460: 81-94.
21. Boukamp P, Petrussevska R T, Breitkreutz D, Hornung J, Markham A, Fusenig N E. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988: 106: 761-771.
22. Gniadecki R. Effects of 1,25-dihydroxyvitamin D3 and its 20-epi analogues (MC 1288, MC 1301, KH 1060), on clonal keratinocyte growth: evidence for differentiation of keratinocyte stem cells and analysis of the modulatory effects of cytokines. Br J Pharmacol 1997: 120: 1119-1127.
23. Gniadecka M, Wulf H C, Mosensen N N, Poulsen T. Photoprotection in vitiligo and normal skin. A quantitative assessment of the role of stratum corneum, viable epidermis and pigmentation. Acta Derm Venereol 1996: 76: 429-432.
24. Kaidbey K H, Agin P P, Sayre R M, Kligman A M. Photoprotection by melanin - a comparison of black and Caucasian skin. J Am Acad Dermatol 1979: 1(3) 249-260.
25. Courdavault S, Baudouin C, Sauvaigo S et al. Unrepaired cyclobutane pyrimidine dimers do not prevent proliferation of UV-B-irradiated cultured human fibroblasts. Photochem Photobiol 2004: 79: 145-151.
26. Ayala-Torres S, Chen Y, Svoboda T, Rosenblatt J, Van Houten B. Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction. Methods 2000: 22: 135-147.