Mammalian mitochondrial endonuclease G: Digestion of R-loops and localization in intermembrane space

European Journal of Biochemistry

Volumn 269, Issue 23, 2002, Pages 5765-5770

  Ohsato, Takashi ^a   Ishihara, Naotada ^a   Muta, Tsuyoshi ^a   Umeda, Shuyo ^a   Ikeda, Shogo ^b   Mihara, Katsuyoshi ^a   Hamasaki, Naotaka ^a   Kang, Dongchon ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b OKAYAMA UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Endonuclease G; Mitochondria; Mitochondrial DNA; R loop; Triplex DNA

Indexed keywords

DNA; DOUBLE STRANDED DNA; ENDONUCLEASE; GUANINE; MITOCHONDRIAL DNA; MITOCHONDRIAL ENZYME; NUCLEASE; PRIMER RNA; RIBONUCLEASE H; RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; DNA CLEAVAGE; DNA REPLICATION; DNA REPLICATION ORIGIN; DNA RNA HYBRIDIZATION; DNA SEQUENCE; DNA STRAND BREAKAGE; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME SUBSTRATE; EXTRACELLULAR MATRIX; HUMAN; MAMMAL; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RAT;

DNA REPLICATION; DNA, MITOCHONDRIAL; ENDODEOXYRIBONUCLEASES; HELA CELLS; HUMANS; HYDROLYSIS; SUBMITOCHONDRIAL PARTICLES;

ANIMALIA; MAMMALIA;

EID: 18744377469     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03238.x     Document Type: Article

References (19)

1. Gerschenson, M., Houmiel, K.L. & Low, R.L. (1995) Endonuclease G from mammalian nuclei is identical to the major endonuclease of mitochondria. Nucleic Acids Res. 23, 88-97.
2. Widlak, P., Li, L.Y., Wang, X. & Garrard, W.T. (2001) Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: Cooperation with exonuclease and DNase I. J. Biol. Chem. 276, 48404-48409.
3. Cote, J. & Ruiz-Carrillo, A. (1993) Primers for mitochondrial DNA replication generated by endonuclease G. Science 261, 765-769.
4. Shadel, G.S. & Clayton, D.A. (1997) Mitochondrial DNA maintenance in vertebrates. Annu. Rev. Biochem. 66, 409-435.
5. Lee, D.Y. & Clayton, D.A. (1997) RNase mitochondrial RNA processing correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication. Genes Dev. 11, 582-592.
6. Zassenhaus, H.P., Hofmann, T.J., Uthayashanker, R., Vincent, R.D. & Zona, M. (1988) Construction of a yeast mutant lacking the mitochondrial nuclease. Nucleic Acids Res. 16, 3282-3296.
7. Li, L.Y., Luo, X. & Wang, X. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria. Nature (London) 412, 95-99.
8. Ohsato, T., Muta, T., Fukuoh, A., Shinagawa, H., Hamasaki, H. & Kang, D. (1999) R-loop in the replication origin of human mitochondrial DNA is resolved by RecG, a Holliday junction-specific helicase. Biochem. Biophys. Res. Commun. 255, 1-5.
9. Lee, D.Y. & Clayton, D.A. (1996) Properties of primer RNA-DNA hybrid at the mouse mitochondrial DNA leading origin of replication. J. Biol. Chem. 271, 24262-24269.
10. Ishihara, N. & Mihara, K. (1998) Identification of the protein import components of the rat mitochondrial inner membrane, rTIM17, rTIM23, and rTIM44. J. Biochem. (Tokyo) 123, 722-732.
11. Iwahashi, J., Yamazaki, S., Komiya, T., Nomura, N., Nishikawa, S., Endo, T. & Mihara, K. (1997) Analysis of the functional domain of the rat liver mitochondrial import receptor Tom20. J. Biol. Chem. 272, 18467-18472.
12. Ruiz-Carrillo, A. & Renaud, J. (1987) Endonuclease G: A (dG) n X (dC) n-specific DNase from higher eukaryotes. EMBO J. 6, 401-407.
13. Ikeda, S. & Ozaki, K. (1997) Action of mitochondrial endonuclease G on DNA damaged by L-ascorbic acid, peplomycin, and cis-diamminedichloroplatinum (II). Biochem. Biophys. Res. Commun. 235, 291-294.
14. Gilbert, D.E. & Feigon, J. (1999) Multistranded DNA structures. Cur R Opinion Sruct. Biol. 9, 305-314.
15. LeProust, E.M., Pearson, C.E., Sinden, R.R. & Gao, X. (2000) Unexpected formation of parallel duplex in GAA and TTC trinucleotide repeats of Friedreich's ataxia. J. Mol. Biol. 302, 1063-1080.
16. Masse, E. & Drolet, M. (1999) Escherichia coli DNA topoisomerase I inhibits R-loop formation by relaxing transcription-induced negative supercoiling. J. Biol. Chem. 274, 16659-16664.
17. Asai, T. & Kogoma, T. (1994) D-loops and R-loops: Alternative mechanisms for the initiation of chromosome replication in Escherichia coli. J. Bacteriol. 176, 1807-1812.
18. Hong, X., Cadwell, G.W. & Kogoma, T. (1995) Escherichia coli RecG and RecA proteins in R-loop formation. EMBO J. 14, 2385-2392.
19. Praseuth, D., Guieysse, A.L. & Helene, C. (1999) Triple helix formation and the antigene strategy for sequence-specific control of gene expression. Biochim. Biophys. Acta. 1489, 181-206.