Assembly of an artificial protein hydrogel through leucine zipper aggregation and bisulfide bond formation

Macromolecules

Volumn 38, Issue 9, 2005, Pages 3909-3916

  Shen, Wei ^a   Lammertink, Rob G H ^a   Sakata, Jill K ^a   Kornfield, Julia A ^a   Tirrell, David A ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIFICATION; ANISOTROPIC SWELLING; ARTIFICIAL PROTEINS; TISSUE ENGINEERING;

AGGLOMERATION; CHEMICAL BONDS; DISSOLUTION; GELATION; HYDROGELS; LIGHT SCATTERING; PH EFFECTS; RHEOLOGY; SWELLING;

PROTEINS;

EID: 18744373049     PISSN: 00249297     EISSN: None     Source Type: Journal    
DOI: 10.1021/ma048348s     Document Type: Article

References (39)

1. Lee, K. Y.; Mooney, D. J. Chem. Rev. 2001, 101, 1869-1879.
2. Gutowska, A.; Jeong, B.; Jasionowski, M. Anatomical Record 2001, 263, 342-349.
3. Delgado, M.; Spanka, C.; Kerwin, L. D.; Wentworth, P.; Janda, K. M. Biomacromolecules 2002, 3, 262-271.
4. Halstenberg, S.; Panitch, A.; Rizzi, S.; Hall, H.; Hubbell, J. A. Biomacromolecules 2002, 3, 710-723.
5. Hisano, N.; Morikawa, N.; Iwata, H.; Ikada, Y. J. Biomed. Mater. Res. 1998, 40, 115-123.
6. Jen, A. C.; Wake, M. C.; Mikos, A. G. Biotechnol. Bioeng. 1996, 50, 357-364.
7. Risbud, M.; Endres, M.; Ringe, J.; Bhonde, R.; Sittinger, M. J. Biomed. Mater. Res. 2001, 56, 120-127.
8. Stile, R. A.; Healy, K. E. Biomacromolecules 2001, 2, 185-194.
9. Martens, P. J.; Bryant, S. J.; Anseth, K. S. Biomacromolecules 2003, 4, 283-292.
10. Petka, W. A.; Harden, J. L.; McGrath, K. P.; Wirtz, D.; Tirrell, D. A. Science 1998, 281, 389-392.
11. Wang, C.; Stewart, R. J.; Kopecek, J. Nature (London) 1999, 397, 417-420.
12. Surks, H. K.; Richards, C. T.; Mendelsohn, M. E. J. Biol. Chem. 2003, 278, 51484-51493.
13. O'Shea, E. K.; Rutkowski, R.; Stafford, W. F.; Kim, P. S. Science 1989, 245, 646-648.
14. O'Shea, E. K.; Klemm, J. D.; Kim, P. S.; Alber, T. Science 1991,254, 539-544.
15. Ishiyama, M.; Shiga, M.; Sasamoto, K.; Mizoguchi, M.; He, P. G. Chem. Pharm. Bull. 1993, 41, 1118-1122.
16. 10Atrp had the same viability as control cells.
17. 2O), and the pH values were adjusted to the range between 7.0 and 8.0. Oscillation frequency sweep tests (from 100 to 0.001 rad/s) were performed at 1% strain on an RFS III rheometer (TA Instruments, New Castle, DE) with a cone-and-plate geometry (0.04 rad cone angle and 25 mm diameter). The storage modulus levels off between 1 and 100 rad/s under all conditions examined (22°C) with values less than 13% of those calculated on the basis of an ideal network assumption where 100% chains are elastically effective, suggesting the predominance of loops.
18. Kennedy, S. B. Ph.D. Dissertation, University of Massachusetts-Amherst, Amherst, MA, 2001.
19. Fluorescein maleimide was site-specifically labeled to the cysteine residue engineered at the end of the leucine zipper A (with a 14-amino acid linker between the zipper domain and the cysteine residue). Fluorescence is self-quenched in this labeled protein solution because of leucine zipper self-assembly. Mixing a labeled leucine zipper solution with an unlabeled leucine zipper solution in a stopped-flow spectrometer led to fluorescence de-quenching, suggesting the strand exchange among leucine zipper aggregates. Details will be reported separately.
20. Xu, B.; Yekta, A.; Li, L.; Masoumi, Z.; Winnik, M. A. Colloids Surf., A 1996, 112, 239-250.
21. Chao, H. M.; Houston, M. E.; Grothe, S.; Kay, C. M.; OconnorMcCourt, M.; Irvin, R. T.; Hodges, R. S. Biochemistry 1996, 35, 12175-12185.
22. Wendt, H.; Berger, C.; Baici, A.; Thomas, R. M.; Bosshard, H. R. Biochemistry 1995, 34, 4097-4107.
23. Kuhn, K.; Glanville, R. W.; Babel, W.; Qian, R. Q.; Dieringer, H.; Voss, T.; Siebold, B.; Oberbaumer, I.; Schwarz, U.; Yamada, Y. Ann. N.Y. Acad. Sci. 1985, 460, 14-24.
24. Yu, J. L.; Yu, D. W.; Checkla, D. M.; Freedberg, I. M.; Bertolino, A. P. J. Invest. Dermatol. 1993, 101, S56-S59.
25. Inoue, S.; Tanaka, K.; Arisaka, F.; Kimura, S.; Ohtomo, K.; Mizuno, S. J. Biol. Chem. 2000, 275, 40517-40528.
26. Zhou, N. E.; Kay, C. M.; Hodges, R. S. Biochemistry 1993, 32, 3178-3187.
27. 10 solutions (140 μM, pH 10.0) were filtered through 0.02 μM filters and subjected to dynamic light scattering measurements on BI-9000AT (Brookhaven) and WyattQELS (Wyatt Technology) digital autocorrelators. The data were analyzed with software (based on the CONTIN algorithm) provided by Brookhaven.
28. Lumb, K. J.; Kim, P. S. Biochemistry 1995, 34, 8642-8648.
29. Petka, W. A. Ph.D. Dissertation, University of Massachusetts-Amherst, Amherst, MA, 1997.
30. Riddles, P. W.; Blakeley, R. L.; Zerner, B. Methods Emymol. 1983, 91, 49-60.
31. Crocker, J. C.; Grier, D. G. J. Colloid Interface Sci. 1998, 179, 298-310.
32. Huglin, M. B. Light Scattering from Polymer Solutions; Academic Press: London, 1972.
33. Jackman, R. J.; Duffy, D. C.; Ostuni, E.; Willmore, N. D.; Whitesides, G. M. Anal. Chem. 1998, 70, 2280-2287.
34. Provencher, S. W.; Glockner, J. Biochemistry 1981, 20, 3337.
35. Kuramitsu, S.; Hamaguchi, K. J. Biochemistry 1979, 18, 443-456.
36. Miller, D. R.; Macosko, C. W. Macromolecules 1976, 9, 206-211.
37. Macosko, C. W.; Miller, D. R. Macromolecules 1976, 9, 199206.
38. Tanaka, T.; Sun, S. T.; Hirokawa, Y.; Katayama, S.; Kucera, J.; Hirose, Y.; Amiya, T. Nature (London) 1987, 325, 796-798.
39. Harmon, M. E.; Tang, M.; Frank, C. W. Polymer 2003, 44, 4547-4556.