Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential

Biological Chemistry

Volumn 383, Issue 9, 2002, Pages 1383-1396

  Stumvoll, Sabina ^a   Lidholm, Jonas ^a   Thunberg, Ronald ^a   DeWitt, Asa Marknell ^a   Eibesteiner, Petra ^a   Swoboda, Ines ^a   Bugajska Schretter, Agnes ^a   Spitzauer, Susanne ^a   Vangelista, Luca ^a   Kazemi Shirazi, Lili ^a   Sperr, Wolfgang R ^a   Valent, Peter ^a   Kraft, Dietrich ^a   Valenta, Rudolf ^a  

^a UNIVERSITY OF VIENNA   (Austria)

Author keywords

Allergy; Circular dichroism spectroscopy; Grass pollen allergen; IgE cross reactivity; Phl p 4

Indexed keywords

HISTAMINE; IMMUNOGLOBULIN E; POLLEN ANTIGEN; PROTEIN PHL P 4; UNCLASSIFIED DRUG; ALLERGEN; PERIODATE; PERIODIC ACID; PHL P 4 PROTEIN, PHLEUM PRATENSE; VEGETABLE PROTEIN;

ALPHA HELIX; ANTIBODY SPECIFICITY; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; BASOPHIL; CIRCULAR DICHROISM; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC TEST; FOOD; GRASS; HISTAMINE RELEASE; HUMAN; IMMUNOLOGY; MOLECULAR WEIGHT; NONHUMAN; PHLEUM PRATENSE; POLLEN ALLERGY; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; RABBIT; SENSITIZATION; SKIN MANIFESTATION; TREE; WEED; ANIMAL; CHEMISTRY; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; POLLEN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; SKIN TEST; WESTERN BLOTTING;

ORYCTOLAGUS CUNICULUS; PHLEUM PRATENSE; POACEAE;

ALLERGENS; ANIMALS; BLOTTING, WESTERN; CIRCULAR DICHROISM; CROSS REACTIONS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTAMINE RELEASE; HUMANS; IMMUNOGLOBULIN E; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; PERIODIC ACID; PHLEUM; PLANT PROTEINS; POLLEN; PROTEIN CONFORMATION; PROTEIN FOLDING; RABBITS; SKIN TESTS;

EID: 18644376280     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2002.157     Document Type: Article

References (47)

1. Ansari, A.A., Shenbagamurthi, P., and Marsh, D.G. (1989). Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III: comparison with known Lol p I and II sequences. Biochemistry 28, 8665-8670.
2. Beaven, M. A., and Metzger, H. (1993). Signal transduction by Fc receptors: the Fc epsilon RI case. Immunol. Today 14, 222-226.
3. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Breiteneder, H., Pettenburger, K., Bito, A., Valenta, R., Kraft, D., Rumpold, H., Scheiner, O., and Breitenbach, M. (1989). The gene coding for the major birch pollen allergen Bet v 1, is highly homologous to a pea disease resistance response gene. EMBO J. 8, 1935-1938.
5. Bufe, A., Spangfort, M. D., Kahlert, H., Schlaak, M., and Becker, W. M. (1996). The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199, 413-415.
6. Dolecek, C., Vrtala, S., Laffer, S., Steinberger, P., Kraft, D., Scheiner, O., and Valenta, R. (1993). Molecular characterization of Phl p II, a major timothy grass (Phleum pratense) pollen allergen. FEBS Lett. 335, 299-304.
7. Ebner, C., Hirschwehr, R., Bauer, L., Breiteneder, H., Valenta, R., Ebner, H., Kraft, D., and Scheiner, O. (1995). Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin). J. Allergy Clin. Immunol. 95, 962-969.
8. Ekramoddoullah, A.K.M., Kisil, F.T., and Sehon, A.H. (1983). Immunochemical characterization of a high molecular weight basic allergen (HMBA) of rye grass (Lolium perenne) pollen. Mol. Immunol. 20, 465-473.
9. Fischer, S., Grote, M., Fahlbusch, B., Müller, W.D., Kraft, D., and Valenta, R. (1996). Characterization of Phl p 4, a major timothy grass (Phleum pratense) pollen allergen. J. Allergy Clin. Immunol. 98, 189-198.
10. Fling, S.P., and Gregerson, D.S. (1986). Peptide and protein molecular weight Determination by electrophoresis using a high-molarity Tris buffer system without urea. Anal. Biochem. 155, 83-88.
11. Freidhoff, L.R., Ehrlich-Kautzky, E., Grant, J.H., Meyers, D.A., and Marsh, D.G. (1986). A Study of the human immune response to Lolium perenne (rye) pollen and its components, Lol p I and Lol p II (rye I and rye II). J. Allergy Clin. Immunol. 78, 1190-201.
12. Jaggi, K.S., Ekramodoullah, A.K., and Kisil, F.T. (1989). Allergenic fragments of ryegrass (Lolium perenne) pollen allergen Lol p IV. Int. Arch. Allergy Appl. Immunol. 89, 342-348.
13. Johnson, P., and Marsh, D.G. (1965). 'Isoallergens' from rye grass pollen. Nature 206, 935-937.
14. Kay, A.B. (1997) Allergy and Allergic Diseases (Oxford, UK: Blackwell Science).
15. King, T.P., Hoffmann, D., Lowenstein, H., Marsh, D.G., Platts-Mills, T.A., and Thomas, W. (1995). Allergen nomenclature (WHO). J. Allergy Clin. Immunol. 96, 4-14.
16. Laffer, S., Valenta, R., Vrtala, S., Susani, M., van Ree, R., Kraft, D., Scheiner, O., and Duchêne, M. (1994). Complementary DNA cloning of the major allergen Phl p 1 from timothy grass (Phleum pratense); recombinant Phl p 1 inhibits IgE-binding to group 1 allergens from eight different grass species. J. Allergy Clin. Immunol. 94, 689-698.
17. Laffer, S., Spitzauer, S., Susani, M., Pairleitner, H., Schweiger, C., Grönlund, H., Menz, G., Pauli, G., Ishii, T., Nolte, H., Ebner, C., Sehon, A. H., Kraft, D., Eichler, H. G., and Valenta, R. (1996a). Comparison of recombinant timothy grass pollen allergens with natural extract for diagnosis of grass pollen allergy in different populations. J. Allergy Clin. Immunol. 98, 652-658.
18. Laffer, S., Vangelista, L., Steinberger, P., Kraft, D., Pastore, A., and Valenta, R. (1996b). Molecular characterization of Bip 1, a monoclonal antibody that modulates IgE binding to birch pollen allergen Bet v 1. J. Immunol. 157, 4943-4962.
19. Leduc-Brodard, V., Inacio, F., Jaquinod, M., Forest, E., David, B., and Peltre, G. (1996). Characterization of Dac g 4, a major basic allergen from Dactylis glomerata pollen. J. Allergy Clin. Immunol. 98, 1065-1072.
20. Mari, A., Iacovacci, P., Afferni, C., Barletta, B., Tinghino, R., Di-Felice, G., and Pini, C. (1999). Specific IgE to cross-reactive carbohydrate determinants strongly affect the in vitro diagnosis of allergic diseases. J. Allergy Clin. Immunol. 103, 1005-1011.
21. Matthiesen, F., and Lowenstein, H. (1991). Group V allergens in grass pollens. II. Investigation of group V allergens in pollens from 10 grasses. Clin. Exp. Allergy 21, 309-330.
22. Matthiesen, F., Friberg, L., Olsen, M., and Lowenstein, H. (1993). Purification and characterization of the Phleum pratense (timothy) pollen allergen Phl p 6. In: Molecular Biology and Immunology of Allergens, A. H. Sehon and D. Kraft, eds. (Boca Raton, FL, USA: CRC Press), pp. 189-191.
23. Mittermann, I., Fetrow, J. S., Schaak, D. L., Almo, S. C., Kraft, D., Heberle-Bors, E., and Valenta, R. (1998). Oligomerization of profilins from birch, man and yeast. Profilin, a ligand for itself? Sex. Plant Reprod. 11, 183-191.
24. Müller, W.D, Fahlbusch, B., Schlenvoigt, G., Wahl, R., and Jäger, L. (1993). Qualitative and quantitative detection of group V and IV grass pollen allergens using specific monoclonal antibodies. In: Molecular Biology and Immunology of Allergens, A. H. Sehon and D. Kraft, eds. (Boca Raton, FL, USA: CRC Press), pp. 185-187.
25. Niederberger, V., Laffer, S., Fröschl, R., Kraft, D., Rumpold, H., Kapiotis, S., Valenta, R., and Spitzauer, S. (1998). IgE antibodies to recombinant pollen allergens (Phl p 1, Phl p 2, Phl p 5 and Bet v 2) account for a high percentage of grass pollen-specific IgE. J. Allergy Clin. Immunol. 101, 258-264.
26. Niederberger, V, Hayek, B., Vrtala, S., Laffer, S., Twardosz, A., Vangelista, L., Sperr, W. R., Valent, P., Rumpold, H., Kraft, D., Ehrenberger, K., Valenta, R., and Spitzauer, S. (1999). Calcium-dependent immunoglobulin E recognition of the apo- and calcium-bound form of a cross-reactive two EF-hand timothy grass pollen allergen, Phl p 7. FASEB J. 13, 843-856.
27. Ortolani, C., Ispano, M., Pastorello, E., Bigi, A., Ansaloni, R. (1988). The oral allergy syndrome. Ann. Allergy 61, 47-52.
28. Petersen, A., Bufe, A., Schramm, G., Schlaak, M., and Becker, W.M. (1995). Characterization of the allergen group VI in timothy grass pollen (Phl p 6) II. cDNA cloning of Phl p 6 and structural comparison to grass group 6 allergens. Int. Arch. Allergy Immunol. 108, 55-59.
29. 2+ binding protein as a new Bermuda grass pollen allergen Cyn d 7: IgE cross-reactivity with oilseed rape pollen allergen Bra r 1. Int. Arch. Allergy Immunol. 114, 265-271.
30. Su, S.N., Lau, G.X., Tsai, J.J., Yang, S.Y, Shen, H.D., Han, S.H. (1991). Isolation and partial characterization of Bermuda grass pollen allergen, BG-60a. Clin. Exp. Allergy 21, 449-455.
31. Suck, R., Petersen, A., Hagen, S., Cromwell, O., Becker, W. M., and Fiebig, H. (2000a). Complementary DNA cloning and expression of a newly recognized high molecular mass allergen Phl p 13 from timothy grass pollen (Phleum pratense). Clin. Exp. Allergy 30, 324-332.
32. Suck, R., Hagen, S., Cromwell, O., and Fiebig, H. (2000b). The high molecular mass allergen fraction of timothy grass pollen (Phleum pratense) between 50-60 kDa is comprised of two major allergens: Phl p 4 and Phl p 13. Clin. Exp. Allergy 30, 1395-1402.
33. Suphioglu, C., Ferreira, F., and Knox, R.B. (1997). Molecular cloning and immunological characterization of Cyn d 7, a novel calcium-binding allergen from Bermuda grass pollen. FEBS Lett. 402, 167-172.
34. Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
35. Valent, P., Besemer, J., Muhm, M., Majdic, O., Lechner, K., and Bettelheim, P. (1989). Interleukin 3 activates human blood basophils via high-affinity binding sites. Proc. Natl. Acad. Sci. USA 86, 5542-5546.
36. Valenta, R., and Kraft, D. (1996). Type I allergic reactions to plant-derived food: a consequence of primary sensitization to pollen allergens. J. Allergy Clin. Immunol. 97, 893-895.
37. Valenta, R., Duchêne, M., Pettenburger, K., Sillaber, C., Valent, P., Bettelheim, P., Breitenbach, M., Rumpold, H., Kraft, D., and Scheiner, O. (1991). Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals. Science 253, 557-560.
38. Valenta, R., Ferreira, F., Grote, M., Swoboda, I., Vrtala, S., Duchêne, M., Deviller, P., Meagher, R.B., McKinney, E., Heberle-Bors, E., Kraft, D., and Scheiner, O. (1993). Identification of profilin as an actin binding protein in higher plants. J. Biol. Chem. 268, 22777-22781.
39. Valenta, R., Ball, T., Vrtala, S., Duchêne, M., Kraft, D., and Scheiner, O. (1994). cDNA cloning and expression of timothy grass (Phleum pratense) pollen profilin in Escherichia coli: comparison with birch pollen profilin. Biochem. Biophys. Res. Commun. 199, 106-118.
40. Valenta, R., Steinberger, P., Duchêne, M., and Kraft, D. (1996). Immunological and structural similarities among allergens: prerequisite for a specific and component-based therapy of allergy. Immunol. Cell Biol. 74, 187-194.
41. Valenta, R., Lidholm, J., Hayek, B., Kraft, D., and Grönlund, H. (1999). The recombinant allergen-based concept of component-resolved diagnostics and immunotherapy. Clin. Exp. Allergy 29, 896-904.
42. van Ree, R., Hoffmann, D.R., van Dijk, W., Brodard, V., Mahieu, K., Koeleman, C.A., Grande, M., van Leeuwen, W.A., and Aalberse, R.C. (1995). Lol p IX, a new major grass pollen allergen, is a member of a family of soybean trypsin inhibitor-related proteins. J. Allergy Clin. Immunol. 95, 970-978.
43. Vieths, S., Jankiewicz, A., Aulepp, H., and Haustein, D. (1997). Allergy to heated and processed foods. Arb. Paul Ehrlich Inst. 91, 250-262.
44. Vrtala, S., Sperr, W.R., Reimitzer, I., van Ree, R., Laffer, S., Müller, W. D., Valent, P., Lechner, K., Rumpold, H., Kraft, D., Scheiner, O., and Valenta, R. (1993). cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen; characterization of the recombinant Phl p V allergen. J. Immunol. 151, 4773-4781.
45. Vrtala, S., Susani, M., Sperr, W.R., Valent, P., Laffer, S., Dolecek, C., Kraft, D., and Valenta, R. (1996). Immunologic characterization of purified recombinant timothy grass pollen (Phleum pratense) allergens (Phl p 1, Phl p 2, Phl p 5). J. Allergy Clin. Immunol. 97, 781-787.
46. Vrtala, S., Hirtenlehner, K., Vangelista, L., Pastore, A., Eichler, H. G., Sperr, W. R., Valent, P., Ebner, C., Kraft, D., and Valenta, R. (1997). Conversion of the major birch pollen allergen, Bet v 1, into two nonanaphylactic T cell epitope-containing fragments: candidates for a novel form of specific immunotherapy. J. Clin. Invest. 99, 1673-1681.
47. Vrtala, S., Fischer, S., Grote, M., Vangelista, L., Pastore, A., Sperr, W.R., Valent, P., Reichelt, R., Kraft, D., and Valenta, R. (1999). Molecular, immunological, and structural characterization of Phl p 6, a major allergen and P-particle-associated protein from timothy grass (Phleum pratense) pollen. J. Immunol. 163, 5489-5496.