Comparison of recombinant timothy grass pollen allergens with natural extract for diagnosis of grass pollen allergy in different populations

Journal of Allergy and Clinical Immunology

Volumn 98, Issue 3, 1996, Pages 652-658

  Laffer, Sylvia ^a   Spitzauer, Susanne ^b   Susani, Markus ^c   Pairleitner, Helmut ^a   Schweiger, Christian ^b   Grönlund, Hans ^d   Menz, Günter ^e   Pauli, Gabrielle ^f   Ishii, Toyota ^g   Nolte, Hendrik ^h   Ebner, Christof ^a   Sehon, Alec H ⁱ   Kraft, Dietrich ^a   Eichler, Hans G ^b   Valenta, Rudolf ^a,b  

^a Institute of General and Experimental Pathology   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

^c Institute of Molecular Biology Salzburg   (Austria)

^d Thermo Fisher Scientific   (United States)

^e Hochgebirgsklinik   (Switzerland)

^f HÔPITAL CIVIL   (France)

^g SAGAMIHARA NATIONAL HOSPITAL   (Japan)

^h University of Copenhagen   (Denmark)

ⁱ Departments of Immunology   (Canada)

Author keywords

grass pollen allergy; in vitro diagnosis; Phl p 1; Phl p 2; Phl p 5; Population study; profilin; recombinant timothy grass pollen allergens

Indexed keywords

ALLERGEN; COMPLEMENTARY DNA; IMMUNOGLOBULIN E; PROFILIN;

ALLERGY; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACT; GRASS; GRASS POLLEN; HUMAN; IMMUNOBLOTTING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RADIOALLERGOSORBENT TEST; RADIOIMMUNOASSAY; SKIN TEST;

EID: 0029960488     PISSN: 00916749     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0091-6749(96)70099-4     Document Type: Article

References (23)

1. Freidhoff LR, Ehrlich-Kautzky E, Grant JH, Meyers DA, Marsh DG. A study of the human immune response to Lolium perenne (rye) pollen and its components, Lol p I and Lol p II (rye I and rye II). J Allergy Clin Immunol 1986;78:1190-201.
2. Laffer S, Valenta R, Vrtala S, et al. Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE-binding to group I allergens from eight different grass species. J Allergy Clin Immunol 1994;94:689-98.
3. Dolecek C, Vrtala S, Laffer S, Steinberger P, Kraft D, Scheiner O, Valenta R. Molecular characterization of Phl p II, a major timothy grass (Phleum pratense) pollen allergen. FEBS Lett 1993;335:299-304.
4. Vrtala S, Sperr WR, Reimitzer I, et al. cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen, Phl p V; characterization of the recombinant Phl p V allergen. J Immunol 1993;151:4773-81.
5. Valenta R, Vrtaia S, Ebner C, Kraft D, Scheiner O. Diagnosis of grass pollen allergy with recombinant timothy grass (Phleum pratense) pollen allergens. Int Arch Allergy Immunol 1992;97:287-94.
6. Laffer S, Vrtala S, Duchêne M, et al. IgE-binding capacity of recombinant timothy grass (Phleum pratense) pollen allergens. J Allergy Clin Immunol 1994;94:88-94.
7. Vrtala S, Susani M, Sperr WR, et al. Immunologic characterization of purified recombinant timothy grass pollen (Phleum pratense) allergens (Phl p 1, Phl p 2, Phl p 5). J Allergy Clin Immunol 1996;97:781-7.
8. Schenk S, Breiteneder H, Susani M, et al. T-cell epitopes of Phl p 1, major pollen allergen of timothy grass (Phleum pratense): evidence for crossreacting and non-crossreacting T-cell epitopes within grass group I allergens. J Allergy Clin Immunol 1995;96:986-96.
9. Laffer S, Duchêne M, Reimitzer I, et al. Common IgE-epitopes of a recombinant major timothy grass pollen allergen, Phl p 1, and natural group 1 grass pollen isoallergens. Mol Immunol 1996;33:417-26.
10. Valenta R, Duchêne M, Pettenburger K, et al. Identification of profilin as a novel pollen allergen: IgE autoreactivity in sensitized individuals. Science 1991;253:557-60.
11. Valenta R, Duchêne M, Ebner C, et al. Profilins constitute a novel family of functional plant pan-allergens. J Exp Med 1995;175:377-85.
12. Valenta R, Ball T, Vrtala S, Duchêne M, Kraft D, Scheiner O. cDNA cloning and expression of timothy grass (Phleum pratense) pollen profilin in Escherichia coli: comparison with birch pollen profilin. Biochem Biophys Res Commun 1994;199:106-18.
13. Vrtala S, Grote M, Duchêne M, et al. Properties of tree and grass pollen allergens: reinvestigation of the linkage between solubility and allergenicity. Int Arch Allergy Immunol 1993;102:160-9.
14. Fling SP, Gregerson DS. Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris buffer system without urea. Anal Biochem 1986; 155:83-8.
15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248-54.
16. Susani M, Jertschin P, Dolecek C, et al. High level expression of birch pollen profilin (Bet v 2) in Escherichia coli; purification and characterization of the recombinant allergen. Biochem Biophys Res Commun 1995;215:250-63.
17. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
18. Fischer S, Grote M, Fahlbusch B, Müller WD, Kraft D, Valenta R. Characterization of Phl p 4, a major timothy grass (Phleum pratense) pollen allergen. J Allergy Clin Immunol 1996;98:189-98.
19. Vrtala S, Susani M, Kraft D, Valenta R. Crossreactivity and different allergenicity of recombinant timothy grass pollen grass allergens, rPhl p 1, rPhl p 2 and rPhl p 5 in mice. Submitted for publication.
20. Breiteneder H, Pettenburger K, Bito A, Breitenbach M. The gene coding for the major birch pollen allergen, Bet v I, is highly homologous to a pea disease resistance response gene. EMBO J 1989;8:1935-8.
21. Vrtala S, Mayer P, Ferreira F, et al. Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: different allergenicity of Bet v 1 and Bet v 2. J Allergy Clin Immunol. In press.
22. Pauli G, Oster JP, Deviller P, et al. Skin testing with recombinant allergens rBet v 1 and birch profilin, rBet v 2: diagnostic value for birch pollen and associated allergies. J Allergy Clin Immunol 1996;97:1100-9.
23. Menz G, Dolecek C, Schönheit-Kenn U, et al. Serological and skin-test diagnosis of birch pollen allergy with recombinant Bet v 1, the major birch pollen allergen. Clin Exp Allergy 1996; 26:50-60.