High-efficiency DNA ligation for clamp attachment without polymerase chain reaction

Analytical Biochemistry

Volumn 310, Issue 2, 2002, Pages 179-185

  Kim, Andrea S ^a,c   Holmquist, Gerald P ^b   Thilly, William G ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^c Fred Hutchinson Cancer Research Center   (United States)

Author keywords

CDCE; Clamp attachment; High efficiency DNA ligation; Mass action; Rare point mutational analysis

Indexed keywords

CAPILLARY ELECTROPHORESIS; DNA; DNA SEQUENCES; EFFICIENCY; OLIGONUCLEOTIDES;

AMPLIFIED FRAGMENTS; CDCE; CONSTANT DENATURANT CAPILLARY ELECTROPHORESIS; DNA LIGATION; HIGH-EFFICIENCY; MASS ACTION; MAXIMUM EFFICIENCY; MUTATIONAL ANALYSIS;

POLYMERASE CHAIN REACTION;

OLIGONUCLEOTIDE; DNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA;

ARTICLE; CAPILLARY ELECTROPHORESIS; DNA DETERMINATION; DNA SEQUENCE; GENE SEQUENCE; PATCH CLAMP; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; CHEMISTRY; DNA BASE COMPOSITION; DNA DENATURATION; FACTUAL DATABASE; GENE AMPLIFICATION; GENETICS; GENOME; HEAT; HUMAN; METABOLISM; METHODOLOGY; NUCLEOTIDE SEQUENCE; OSMOLARITY; SENSITIVITY AND SPECIFICITY;

BASE COMPOSITION; BASE SEQUENCE; DATABASES, FACTUAL; DNA; DNA LIGASES; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DNA, NEOPLASM; ELECTROPHORESIS, CAPILLARY; GENE AMPLIFICATION; GENOME, HUMAN; HEAT; HUMANS; NUCLEIC ACID DENATURATION; OLIGONUCLEOTIDES; OSMOLAR CONCENTRATION; POINT MUTATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 1842854970     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00383-4     Document Type: Article

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