Modular structure, local flexibility and cold-activity of a novel chitobiase from a psychrophilic antarctic bacterium

Journal of Molecular Biology

Volumn 310, Issue 2, 2001, Pages 291-297

  Lonhienne, Thierry ^a,b   Zoidakis, Jérôme ^a,b   Vorgias, Constantinos E ^c   Feller, Georges ^a   Gerday, Charles ^a   Bouriotis, Vassilis ^b  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY   (Greece)

^c UNIVERSITY OF ATHENS   (Greece)

Author keywords

Chitobiase; Extremophile; Glycosyl hydrolase; Microcalorimetry; Psychrophile

Indexed keywords

CALCIUM ION; GALACTOSE; GLYCOSIDASE; IMMUNOGLOBULIN; N,N' DIACETYL CHITOBIOSE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTHROBACTER; ARTICLE; BACTERIUM ADHERENCE; BINDING SITE; CATALYSIS; COLD; DIFFERENTIAL SCANNING CALORIMETRY; ENERGY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; LOW TEMPERATURE; MICROCALORIMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; THERMODYNAMICS;

ARTHROBACTER; ARTHROBACTER SP.; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 0035816221     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4774     Document Type: Article

References (34)

1. The occurrence and characteristics of chitinoclastic bacteria in the sea
2. Chitin utilization by marine bacteria: A physiologycal function for bacteria adhesion to immobilized carbohydrates
3. Analytical biochemistry and clinical significance of N-acetyl-beta-D-glucosaminidase and related enzymes
4. Molecular cloning and expression of the Candida albicans β-N-acetylglucosaminidase (HEX1) gene
5. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities
6. Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease
7. Cloning, sequences, and characterization of two chitinase genes from the Antarctic Arthrobacter sp. strain TAD20: Isolation and partial characterization of the enzymes
8. Enzyme activity determination on macromolecular substrates by isothermal titration calorimetry: Application to mesophilic and psychrophilic chitinases
9. Proteins and temperature
10. Psychrophilic enzymes: Molecular basis of cold adaptation
11. Basic local alignment search tool
12. Rapid and sensitive sequence comparison with FASTP and FASTA
13. Structural and functional characterization of Streptomyces plicatus β-N-acetylhexosaminidase by comparative molecular modeling and site-directed mutagenesis
14. Structures of chitobiase mutants complexed with the substrate di-N-acetyl-D-glucosamine: The catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540
15. The three domains of a bacterial sialidase: A beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll
16. Structure of cyclodextrin glycosyltransferase
17. Domains in microbial β-1,4-glycanases: Sequence conservation, function, and enzyme families
18. Cell-wall-associated proteins in Gram-positive bacteria
19. gamma-Glutamyltranspeptidase from Escherichia coli K-12: Formation and localization
20. N-Acetylglucosaminidase (chitobiase) from Serratia marcescens, gene sequence, protein production and purification in Escherchia coli
21. Stability of proteins: Proteins which do not present a single cooperative system
22. Quantitative analysis of differential scanning calorimetry data
23. Molecular evolution of bacterial cell-surface proteins
24. Study of the glycosidases of jack bean meal
25. Purification, properties, kinetics, and mechanism of β-N-acetyl-glucosamidase from Aspergillus niger
26. Glycosidases of Aspergillus niger
27. Study of Turbatix aceti β-N-acetylglucosaminidase
28. Thermostable, salt tolerant, wide pH range novel chitobiase from Vibrio parahemolyticus: Isolation, characterization, molecular cloning and expression
29. The chitin catabolic cascade in the marine bacterium Vibrio furnissii
30. Hot spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes
31. Psychrophilic enzymes: Revisiting the thermodynamic parameters of activation may explain local flexibility
32. Thermodynamic stability of a cold-active α-amylase from the Antarctic bacterium Alteromonas haloplanctis
33. Calculation of protein extinction coefficients from amino acid sequence data