Altered flexibility in the substrate-binding site of related native and engineered high-alkaline Bacillus subtilisins

Journal of Molecular Biology

Volumn 292, Issue 1, 1999, Pages 111-123

  Mulder, Frans A A ^a   Schipper, Dick ^b   Bott, Richard ^c   Boelens, Rolf ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b Gist Brocades NV   (Netherlands)

^c GENENCOR INTERNATIONAL INC   (United States)

Author keywords

15N relaxation; NMR; Protein dynamics; Protein engineering; Serine protease

Indexed keywords

ALANINE; AMIDE; AMINO ACID; DETERGENT; NITROGEN 15; SERINE PROTEINASE; SUBTILISIN; THREONINE;

ARTICLE; BINDING SITE; CATALYSIS; EGGERTHELLA LENTA; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; GENETIC ENGINEERING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN STABILITY; PROTEIN STRUCTURE; X RAY CRYSTALLOGRAPHY;

BACILLUS LENTUS;

EID: 0033543577     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3034     Document Type: Article

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