Isocitrate dehydrogenase from Thermus aquaticus YT1: Purification of the enzyme and cloning, sequencing, and expression of the gene

Applied and Environmental Microbiology

Volumn 62, Issue 12, 1996, Pages 4627-4631

  Miyazaki, Kentaro ^a  

^a NATL INST BIOSCI HUM TECHNOL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; DNA; ISOCITRATE DEHYDROGENASE; NUCLEOTIDE; OLIGONUCLEOTIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; COLUMN CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; GENE; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; SEQUENCE HOMOLOGY; THERMUS AQUATICUS;

ANABAENA SP.; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); COLI BACILLUS; ESCHERICHIA COLI; THERMUS AQUATICUS; THERMUS THERMOPHILUS; VIBRIO SP.;

EID: 0029967931     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.12.4627-4631.1996     Document Type: Article

References (17)

1. Asakura, K., H. Komatsubara, S. Soga, T. Yomo, M. Oka, S. Emi, and I. Urabe. 1993. Cloning, nucleotide sequence, and expression in Escherichia coli of DNA polymerase gene (polA) from Thermus thermophilus HB8. J. Ferment. Bioeng. 76:265-269.
2. Date, T., K. Tanihara, and N. Numura. 1990. Construction of Escherichia coli vectors for expression and mutagenesis: synthesis of human c-Myc protein that is initiated at a non-AUG codon in exon 1. Gene 99:141-144.
3. Dean, A. M., and D. E. Koshland, Jr. 1993. Kinetic mechanism of Escherichia coli isocitrate dehydrogenase. Biochemistry 32:9302-9309.
4. Eguchi, H., T. Wakagi, and T. Oshima. 1989. A highly stable NADP-dependent isocitrate dehydrogenase from Thermus thermophilus HB8: purification and general properties. Biochim. Biophys. Acta 990:133-137.
5. Hurley, J. H., P. E. Thorsness, V. Ramalingam, N. H. Helmers, D. E. Koshland, Jr., and R. M. Stroud. 1989. Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proc. Natl. Acad. Sci. USA 86:8635-8639.
6. Ishii, A., M. Suzuki, T. Sahara, Y. Takada, S. Sasaki, and N. Fukunaga. 1993. Genes encoding two isocitrate dehydrogenase isozymes of a psychrophilic bacterium. Vibrio sp. strain ABE-1. J. Bacteriol. 175:6873-6880.
7. Jin, S., and A. L. Sonenshein. 1994. Identification of two distinct Bacillus subtilis citrate synthase genes. J. Bacteriol. 176:4669-4679.
8. Kagawa, Y., H. Nojima, N. Nukiwa, M. Ishizuka, T. Nakajima, T. Yasuhara, T. Tanaka, and T. Oshima. 1984. High guanine plus cytosine content in the third letter of codons of an extreme thermophile. J. Biol. Chem. 259:2956-2960.
9. Kirino, H., and T. Oshima. 1991. Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile, Thermus aquaticus YT-1. J. Biochem. 109:852-857.
10. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
11. Miyazaki, K., H. Eguchi, A. Yamagishi, T. Wakagi, and T. Oshima. 1992. Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8. Appl. Environ. Microbiol. 58:93-98.
12. +-isocitrate dehydrogenase from the cyanobacterium Anabaena sp. strain PCC 7220: purification and characterization of the enzyme and cloning, sequencing, and disruption of the icd gene. J. Bacteriol. 176:2718-2726.
13. Oshima, T., and K. Imahori. 1974. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24:102-112.
14. Ramaley, R. F., and M. O. Hudock. 1973. Purification and properties of isocitrate dehydrogenase (NADP) from Thermus aquaticus YT-1, Bacillus subtilis-168 and Chlamydomonas reinhardti-Y-2. Biochim. Biophys. Acta 315: 22-36.
15. Saito, H., and K. Miura. 1963. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619-624.
16. Sanger, F., and A. R. Coulson. 1977. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94:441-448.
17. Thorsness, P. E., and D. E. Koshland, Jr. 1987. Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate. J. Biol. Chem. 262:10422-10425.