Solubilization of Serotonin1A Receptors Heterologously Expressed in Chinese Hamster Ovary Cells

Cellular and Molecular Neurobiology

Volumn 24, Issue 2, 2004, Pages 293-300

  Chattopadhyay, Amitabha ^a   Jafurulla, Md ^a   Kalipatnapu, Shanti ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

5 HT1A receptors; 8 OH DPAT; CHAPS; CHO cells; Heterologous expression; Solubilization

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; GUANINE NUCLEOTIDE BINDING PROTEIN; MACROGOL; MEMBRANE RECEPTOR; NEUROTRANSMITTER RECEPTOR; SEROTONIN 1A RECEPTOR; SODIUM CHLORIDE; 3 ((3 CHOLAMIDOPROPYL)DIMETHYLAMMONIUM) 1 PROPANESULFONATE; 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIUM)-1-PROPANESULFONATE; CHOLIC ACID DERIVATIVE; MACROGOL DERIVATIVE; SEROTONIN;

ANIMAL CELL; ARTICLE; ASSAY; BEHAVIOR; CHO CELL; COGNITION; LIGAND BINDING; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; SOLUBILIZATION; ANIMAL; BINDING COMPETITION; BIOASSAY; BIOLOGICAL MODEL; CELL MEMBRANE; CHEMISTRY; DRUG EFFECT; GENETICS; HAMSTER; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NEUROCHEMISTRY; PHYSIOLOGY; PRECIPITATION; SOLUBILITY; SYNAPTIC TRANSMISSION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; BINDING, COMPETITIVE; BIOLOGICAL ASSAY; CELL MEMBRANE; CHO CELLS; CHOLIC ACIDS; CRICETINAE; MODELS, BIOLOGICAL; NEUROCHEMISTRY; POLYETHYLENE GLYCOLS; PRECIPITATION; RECEPTOR, SEROTONIN, 5-HT1A; SEROTONIN; SODIUM CHLORIDE; SOLUBILITY; SYNAPTIC TRANSMISSION;

EID: 1842732262     PISSN: 02724340     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:CEMN.0000018623.81954.39     Document Type: Article

References (38)

1. Aguilar, J. S., and Ochoa, E. L. M. (1986). The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors. Neurochem. Int. 8:47-51.
2. 1A receptor gene. J. Biol. Chem. 265:5825-5832.
3. 1A antagonists. Trends Neurosci. 19:378-383.
4. Banerjee, P. (1999). Detergents. In Creighton, T. E. (ed.), Encyclopedia of Molecular Biology, Wiley, New York, pp. 661-666.
5. 1A receptor in neural and non-neural cell lines. Biochem. Biophys. Res. Commun. 192:104-110.
6. Banerjee, P., Joo, J. B., Buse, J. T., and Dawson, G. (1995). Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem. Phys. Lipids 77:65-78.
7. Bruns, R. F., Lawson-Wendling, K., and Pugsley, T. A. (1983). A rapid filtration assay for soluble receptors using polyethylenimine-treated filters. Anal. Biochem. 132:74-81.
8. Chattopadhyay, A., and Harikumar, K. G. (1996). Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization. FEBS Lett. 391:199-202.
9. 1A receptors from bovine hippocampus using pre-micellar CHAPS at low concentration. Mol. Membr. Biol. 19:211-220.
10. Chattopadhyay, A., Rukmini, R., and Mukherjee, S. (1996). Photophysics of a neurotransmitter: Ionization and spectroscopic properties of serotonin. Biophys. J. 71:1952-1960.
11. 1A receptor. Nature 335:358-360.
12. Fraser, C. M. (1990). Expression of receptor genes in cultured cells. In Hulme, E. C. (ed.), Receptor Biochemistry: A Practical Approach, IRL Press, New York, pp. 263-275.
13. Gal, A., Braun, S., Feder, D., and Levitzki, A. (1983). Reconstitution of a functional β-adrenergic receptor using cholate and a novel method for its functional assay. Eur. J. Biochem. 134:391-396.
14. Garavito, R. M., and Ferguson-Miller, S. (2001). Detergents as tools in membrane biochemistry. J. Biol. Chem. 276:32403-32406.
15. 3H-PAT. Nature 305:140-142.
16. 1A receptors from bovine hippocampus. Cell. Mol. Neurobiol. 18:535-553.
17. Harikumar, K. G., and Chattopadhyay, A. (1998b). Modulation of agonist and antagonist interactions in serotonin 1A receptors by alcohols. FEBS Lett. 438:96-100.
18. 1A receptors from bovine hippocampus by anagonist and an antagonist. FEBS Lett. 457:389-392.
19. 1A receptors from bovine hippocampus. Brain Res. Bull. 52:597-601.
20. 1A receptors from bovine hippocampus by metal ions. Cell. Mol. Neurobiol. 21:453-464.
21. 1A receptors from bovine hippocampus. Cell. Mol. Neurobiol. 20:665-681.
22. 1A receptor mutant mice. Proc. Natl. Acad. Sci. U.S.A. 95:15049-15054.
23. Hjelmeland, L. M. (1980). A nondenaturing zwitterionic detergent for membrane biochemistry: Design and synthesis. Proc. Natl. Acad. Sci. U.S.A. 77:6368-6370.
24. Hjelmeland, L. M., and Chrambach, A. (1984). Solubilization of functional membrane proteins. Methods. Enzymol. 104:305-318.
25. Hjelmeland, L. M., Nebert, D. W., and Osborne, J. C. (1983). Sulfobetaine derivatives of bile acids: Nondenaturing surfactants for membrane biochemistry. Anal. Biochem. 130:72-82.
26. Jones, O. T., Earnest, J. P., and McNamee, M. G. (1987). Solubilization and reconstitution of membrane proteins. In Findlay, J. B. C., and Evans, W. H. (eds.), Biological Membranes: A Practical Approach, IRL Press, Oxford, pp. 139-177.
27. Julius, D. (1998). Serotonin receptor knockouts: A moody subject. Proc. Natl. Acad. Sci. U.S.A. 95:15153-15154.
28. 2-adrenergic receptors from rat and calf brain. Eur. J. Biochem. 138:573-577.
29. Medrano S., Aguilar, J. S., Rivas, E., and Ochoa, E. L. M. (1989). Incorporation of the nicotinic acetylcholine receptor into liposomes by rapid removal of detergent using poly (ethylene) glycol 6000. Neurochem. Int. 14:65-72.
30. 1A 5-hydroxytryptamine receptors in Chinese hamster ovary cells. Biochem. J. 285:933-938.
31. Ofri, D., Ritter, A. M., Liu, Y., Gioannini, T. L., Hiller, J. M., and Simon, E. J. (1992). Characterization of solubilized opioid receptors: reconstitution and uncoupling of guanine nucleotide-sensitive agonist binding. J. Neurochem. 58:628-635.
32. Ramboz, S., Oosting, R., Amara, D. A., Kung, H. F., Blier, P., Mendelsohn, M., Mann, J-J., Brunner, D., and Hen, R. (1998). Serotonin receptor 1A knockout: An animal model of anxiety-related disorder. Proc. Natl. Acad. Sci. U.S.A. 95:14476-14481.
33. Rivnay, B., and Metzger, H. (1982). Reconstitution of the receptor for immunoglobulin E into liposomes. Conditions for incorporation of the receptor into vesicles. J. Biol. Chem. 257:12800-12808.
34. 1A receptor in neuronal cells during prolonged stress and degeneration. J. Neurochem. 66:2361-2372.
35. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985). Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
36. Strader, C. D., Fong, T. M., Graziano, M. P., and Tota, M. R. (1995). The family of G-protein-coupled receptors. FASEB J. 9:745-754.
37. Tate, C. G., and Grisshammer, R. (1996). Heterologous expression of G-protein-coupled receptors. Trends Biotech. 14:426-430.
38. 2C serotonin receptors. Nature 374:542-546.