Androgen Responsiveness of Renilla Luciferase Reporter Vectors Is Promoter, Transgene, and Cell Line Dependent

Prostate

Volumn 59, Issue 2, 2004, Pages 115-119

  Mulholland, David J ^a   Cox, Michael ^a   Read, Jason ^a   Rennie, Paul ^a   Nelson, Colleen ^a  

^a Jack Bell Research Center   (Canada)

Author keywords

Androgen; Prostate cancer; Renilla luciferase; Transcription

Indexed keywords

ANDROGEN RECEPTOR; ANDROSTANOLONE; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR; LIGAND; LUCIFERASE; PLASMID DNA; RENILLA LUCIFERIN 2 MONOOXYGENASE; THYMIDINE KINASE; UNCLASSIFIED DRUG;

ANDROGEN METABOLISM; ARTICLE; CANCER CELL; CELL LINE; CLONING VECTOR; DATA ANALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; GENETIC TRANSFECTION; HERPES SIMPLEX VIRUS; INHIBITION KINETICS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROSTATE CANCER; PROTEIN EXPRESSION; REGULATORY MECHANISM; RENILLA LUCIFERASE REPORTER VECTOR; RNA TRANSLATION; TRANSCRIPTION REGULATION; TRANSGENE; VALIDATION PROCESS;

ANDROGENS; ANIMALS; ANTHOZOA; BIOLOGICAL ASSAY; GENES, REPORTER; HUMANS; LUCIFERASES; MALE; PROSTATIC NEOPLASMS; REPRODUCIBILITY OF RESULTS; TRANSCRIPTION, GENETIC; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 1842732155     PISSN: 02704137     EISSN: None     Source Type: Journal    
DOI: 10.1002/pros.20059     Document Type: Article

References (4)

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2. Behre G, Smith LT, Tenen DG. Use of a promoterless Renilla luciferase vector as an internal control plasmid for transient cotransfection assays of Ras-mediated transcription activation. Biotechniques 1999;26(1):24-26.
3. Mulholland DJ, Read JT, Rennie PS, Cox ME, Nelson CC. Functional localization and competition between the androgen receptor and T-cell factor for nuclear β-catenin: A means for inhibition of the Tcf signaling axis. Oncogene 2003;22(36):5602-5613.
4. Liao X, Thrasher JB, Pelling J, Holzbeierlein J, Sang QX, Li B. Androgen stimulates matrix metalloproteinase-2 expression in human prostate cancer. Endocrinology 2003;144(5):1656-1663.