Use of a promoterless Renilla luciferase vector as an internal control plasmid for transient Co-transfection assays of Ras-mediated transcription activation

BioTechniques

Volumn 26, Issue 1, 1999, Pages 24-28

  Behre, Gerhard ^a   Smith, Laura T ^a   Tenen, Daniel G ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b HARVARD INSTITUTES OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE;

ARTICLE; BIOASSAY; CONTROL; EXPRESSION VECTOR; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; ONCOGENE RAS; PLASMID; PROMOTER REGION; TECHNIQUE; TRANSACTIVATION;

RENILLA; RENILLA LUCIFERASE;

EID: 0032919897     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99261bm03     Document Type: Article

References (15)

1. Behre, G., L.T. Smith, M.P. Coghlan, T. Hoang, C.L. Carpenter and D.G. Tenen. 1997. Ras enhances the ability of PU.1 to activate the MCSF receptor promoter via c-JUN. Blood 90.(Suppl 1):1363.
2. Farr, A. and A. Roman. 1992. A pitfall of using a second plasmid to determine transfection efficiency. Nucleic Acids Res. 20:920.
3. Feig, L.A. 1988. Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP. Mol. Cell Biol. 8:3235-3243.
4. Friedman, A.D., W.H. Landschulz and S.L. McKnight. 1989. CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. Genes Dev. 3:1314-1322.
5. Fuks, F., L. Deleu, C. Dinsart, J. Rommelaere and S. Faisst. 1996. Ras oncogene-dependent activation of the P4 promoter of minute virus of mice through a proximal P4 element interacting with the Ets family of transcription factors. J. Virol. 70:1331-1339.
6. Hauser, C.A., J.K. Westwick and L.A. Quilliam. 1995. Ras-mediated transcription activation: analysis by transient cotransfection assays. Methods Enzymol. 255:412-426.
7. Lorenz, W.W., M.J. Cormier, D.J. O'Kane, D. Hua, A.A. Escher and A.A. Szalay. 1996. Expression of the Renilla reniformis luciferase gene in mammalian cells. J. Biolumin. Chemilumin. 11:31-37.
8. Lorenz, W. W., R.O. McCann, M. Longiaru and M.J. Cormier. 1991. Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl. Acad. Sci. USA 88:4438-4442.
9. Luckow, B. and G. Schutz. 1987. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15:5490.
10. Nordeen, S.K. 1988. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6:454-458.
11. Promega Corporation. 1997. Dual-Luciferase Reporter Assay System Technical Manual, p. 1-22. Technical Manual No. TM040. Madison, WI.
12. Smeal, T., B. Binetruy, D.A. Mercola, M. Birrer and M. Karin. 1991. Oncogenic and transcriptional cooperation with Ha-ras requires phosphorylation of c-Jun on serines 63 and 73. Nature 354:494-496.
13. Smith, L.T., G. Behre, A. Couvillon, C.L. Carpenter, A.D. Friedman and D.G. Tenen. 1997. Activation of C/EBPalpha transcription factor by ras is attenuated by Myb. Blood 90 (Suppl 1):742.
14. Smith, L.T., S. Hohaus, D.A. Gonzalez, S.E. Dziennis and D.G. Tenen. 1996. PU.1 (Spi1) and C/EBPα regulate the granulocyte colony-stimulating factor receptor promoter in myeloid cells. Blood 88:1234-1247.
15. Zhang, D.-E., C.J. Hetherington, H.M. Chen and D.G. Tenen. 1994. The macrophage transcription factor PU.1 directs tissue specific expression of the macrophage colony stimulating factor receptor. Mol. Cell Biol. 14:373-381.