Phenotypic and genotypic analysis of rhizobia isolated from pasture legumes native of Sardinia and Asinara Island

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 85, Issue 2, 2004, Pages 115-127

  Safronova, V I ^a   Piluzza, G ^b   Belimov, A A ^a   Bullitta, S ^b  

^a ARRIAM   (Russian Federation)

^b CNR   (Italy)

Author keywords

Genotypic characterization; Numerical analysis; Pasture legumes; Rhizobia

Indexed keywords

AMPICILLIN; CARBON; DNA 16S; GENTAMICIN; KANAMYCIN; NITROGEN; PENICILLIN G; RIFAMPICIN; SODIUM CHLORIDE; STREPTOMYCIN; TETRACYCLINE;

GENOTYPE; LEGUME; PASTURE; PHENOTYPE; RHIZOBACTERIUM;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BRADYRHIZOBIUM JAPONICUM; CARBON SOURCE; CLUSTER ANALYSIS; CONTROLLED STUDY; GENOTYPE PHENOTYPE CORRELATION; LEGUME; LINKAGE ANALYSIS; LOTUS; NONHUMAN; PASTURE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RHIZOBIUM; RHIZOBIUM LEGUMINOSARUM; SEQUENCE HOMOLOGY; SINORHIZOBIUM MELILOTI; SPECIES DIFFERENCE; STRAIN DIFFERENCE; SYMBIOSIS; TAXONOMY; TEMPERATURE SENSITIVITY;

DNA, BACTERIAL; DNA, RIBOSOMAL; DRUG RESISTANCE, BACTERIAL; FABACEAE; GENOTYPE; ITALY; MEDITERRANEAN ISLANDS; MICROBIAL SENSITIVITY TESTS; PHENOTYPE; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RESTRICTION MAPPING; RHIZOBIACEAE; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SYMBIOSIS;

EURASIA; EUROPE; ITALY; SARDINIA; SOUTHERN EUROPE;

BRADYRHIZOBIUM; BRADYRHIZOBIUM JAPONICUM; HEDYSARUM; HEDYSARUM CORONARIUM; LOTUS; LOTUS EDULIS; LOTUS ORNITHOPODIOIDES; MESORHIZOBIUM; MESORHIZOBIUM CICERI; MESORHIZOBIUM LOTI; MESORHIZOBIUM MEDITERRANEUM; ORNITHOPUS; ORNITHOPUS COMPRESSUS; RHIZOBIUM; RHIZOBIUM LEGUMINOSARUM; SCORPIURUS; SCORPIURUS MURICATUS; SINORHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 1842689023     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:ANTO.0000020278.58236.77     Document Type: Article

References (36)

1. Ballard R.A. 1996. Assessment of strains of Bradyrhizobium sp. (Lupinus) for serradellas (Ornithopus spp.). Aust. J. Exp. Agric. 36: 63-70.
2. Barry T., Colleran G., Glennon M., Dunican L.K. and Gannon F. 1991. The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Meth. Appl. 1: 51-56.
3. Chen W.X., Li G.S., Qi Y.L., Wang E.T., Yuan H.L. and Li J.L. 1991. Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int. J. Syst. Bacteriol. 41: 275-280.
4. Chen W.X., Wang E., Wang S., Li Y., Chen X. and Li Y. 1995. Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People's Republic of China. Int. J. Syst. Bacteriol. 45: 153-159.
5. Doignon-Bourcier F., Willems A., Coopman R., Laguerre G., Gillis M. and de Lajudie P. 2000. Genotypic characterization of Bradyrhizobium strains nodulating small Senegalese legumes by 16S-23S rRNA intergenic gene spacers and amplified fragment length polymorphism fingerprint analyses. Appl. Environ. Microbiol. 66 (9): 3987-3997.
6. Doyle J.J. 1994. Phylogeny of the legume family: An approach to understanding the origins of nodulation. Annu. Rev. Ecol. Syst. 25: 325-349.
7. Fulchieri M.M., Estrella M.J. and Iglesias A.A. 2001. Characterization of Rhizobium loti strains from the Salado River Basin. Antonie van Leeuwenhoek 79: 119-125.
8. Heyndrickx M., Vauterin L., Vandamme P., Kersters K. and De Vos P. 1996. Applicability of combined amplified ribosomal DNA restriction analysis (ARDRA) patterns in bacterial phylogeny and taxonomy. J. Microbiol. Meth. 26 (3): 247-259.
9. Jarabo-Lorenzo A., Velazquez E., Perez-Galdona R., Vega-Hernandez M.C., Martinez-Molina E., Mateos P.E., Vinuesa P., Martinez-Romero E. and Leon-Barrios M. 2000. Restriction fragment length polymorphism analysis of 16S rDNA and low molecular weight RNA profiling of rhizobial isolates from shrubby legumes endemic to the Canary islands. Syst. Appl. Microbiol. 23: 418-425.
10. Jarvis B.D.W., Pankhurst C.E. and Patel J.J. 1982. Rhizobium loti, a new species of legume root nodule bacteria. Int. J. Syst. Bacteriol. 32: 378-380.
11. Laguerre G., Mavingui P., Allard M.R., Charnay M.P., Louvrier P., Mazurier S.I., Rigottier-Gois L. and Amarger N. 1996. Typing of rhizobia by PCR and PCR-restriction fragment length polymorphism analysis of chromosomal and symbiotic gene regions: Application to Rhizobium leguminosarum and its different biovars. Appl. Environ. Microbiol. 62: 2029-2036.
12. Laguerre G., Van Berkum P., Amarger N. and Prevost D. 1997. Genetic diversity of rhizobial symbionts isolated from legume species within the genera Astragalys, Oxytropis and Onobrychis. Appl. Environ. Microbiol. 63: 4748-4758.
13. Lindström K., Laguerre G., Normand P., Rasmussen U., Heulin T., Jarvis B.D.W., de Lajudie P., Martinez-Romero E. and Chen W.X. 1998. Taxonomy and phylogeny of diazotrophs. In: Elmerich C., Kondorosi A. and Newton W.E. (eds), Biological Nitrogen Fixation for the 21st Century. Proceedings of the 11th International Congress on Nitrogen Fixation, Paris, July 1997. Kluwer Academic Publishers, Dordrecht, pp. 559-570.
14. Lindström K. and Lehtomäki S. 1988. Metabolic properties, maximum growth temperature and phage sensitivity of Rhizobium sp. (Galega) compared with other fast-growing rhizobia. FEMS Microbiol. Lett. 50: 277-287.
15. Lindström K., van Berkum P., Gillis M., Martinez E., Novikova A. and Jarvis B. 1995. Report from the roundtable on Rhizobium taxonomy. In: Tikhonovich I.A. et al. (eds), Nitrogen Fixation: Fundamentals and Applications. Kluwer Academic Publishers, Dordrecht, pp. 807-810.
16. Martinez-Romero E. and Caballero-Mellado J. 1996. Rhizobium phylogenesis and bacterial genetic diversity. Crit. Rev. Plant Sci. 15: 113-140.
17. Nick G., de Lajudie P., Eardly B.D., Suomalainen S., Paulin L., Zhang X., Gillis M. and Lindstrom K. 1999. Sinorhizobium arbores sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya. Int. J. Syst. Bacteriol. 49: 1359-1368.
18. Novikova N. and Safronova V. 1992. Transconjugants of Agrobacterium radiobacter harbouring sym genes of Rhizobium galegae can form an effective symbiosis with Medicago sativa. FEMS Microbiol. Lett. 93: 261-268.
19. Novikova N.I., Pavlova E.A. and Limeshchenko E.V. 1993. Phage sensitivity and host range of Rhizobium strains isolated from root nodules of temperate legumes. Plant Soil 151: 45-53.
20. Oyaizu H., Matsumoto S., Minamisawa K. and Gamou T. 1993. Distribution of rhizobia in leguminous plants surveyed by phylogenetic identification. J. Gen. Appl. Microbiol. 39: 339-354.
21. Pankhurst C.E., Hopcroft D.H. and Jones W.T. 1987. Comparative morphology and flavolan content of Rhizobium loti induced effective and ineffective root nodules on Lotus species, Leucaena leucocephala, Carmichaelia flagelliformis, Ornithopus sativus and Clianthus puniceus. Can. J. Bot. 65: 2676-2685.
22. Paul E.A. and Clark F.E. 1989. Soil Microbiology and Biochemistry, Academic Press, Inc., London.
23. Safronova V. and Novikova N. 1996. Comparison of two methods for root nodule bacteria preservation: Lyophilization and liquid nitrogen freezing. J. Microbiol. Meth. 24: 231-237.
24. Sambrook J., Fritsch E.F. and Maniatis T.A. 1989. Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
25. Squartini A., Struffi P., Doring H., Selenska-Pobell S., Tola E., Giacomini A., Vendramin E., Velazquez E., Mateos P.F., Martinez-Molina E., Dazzo F.B., Casella S. and Nuti M.P. 2002. Rhizobium sullae sp. nov. (formerly 'Rhizobium hedysari'), the root-nodule microsymbiont of Hedysarum coronarium L. Int. J. Syst. Evol. Microbiol. 52: 1267-1276.
26. Sneath P.H.A. and Sokal R.B. 1973. Numerical Taxonomy. The Principles and Practices of Numerical Classification. Freeman W.H. and Co., San Francisco.
27. Sy A., Giraud E., Jourand P., Garcia N., Willems A., de Lajudie P., Prin Y., Neyra M., Gillis M., Boivin-Masson C. and Dreyfus B. 2001. Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. J. Bacteriol. 183: 214-220.
28. Trinick M.J. 1982. In: Broughton W.J. (ed.), Nitrogen Fixation. Vol. 2:. Clarendon Press, Oxford, pp. 76-146.
29. Turner G.L. and Gibson A.H. 1980. Measurement of nitrogen fixation by indirect means. In: Bergensen F.J. (ed.), Methods for Evaluating Biological Nitrogen Fixation. Wiley, Toronto, pp. 111-138.
30. van Rhijn P. and Vanderleyden J. 1995. The Rhizobium-plant symbiosis. Microbiol. Rev. 59: 124-142.
31. Vincent J.M. 1970. A manual for the practical study of root nodule bacteria. IBP Handbook No. 15. Blackwell Scientific Publications, Oxford and Edinburgh, pp. 73-97.
32. Vinuesa P., Rademaker J.L.W., de Bruijn F.J. and Werner D. 1998. Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extragenic palindromic PCR genomic fingerprinting and partial 16S rDNA sequencing. Appl. Environ. Microbiol. 64: 2096-2104.
33. Wang E.T., van Berkum P., Sui X.H., Beyene D., Chen W.X. and Martinez-Romero E. 1999. Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int. J. Syst. Bacteriol. 49: 51-65.
34. Weisburg W.G., Barns S.M., Pelletier D.A. and Lane D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173: 697-703.
35. Woese C.R. 1987. Bacterial evolution. Microbiol. Rev. 51: 221-271.
36. Young J.M., Kuykendall L.D., Martinez-Romero E., Kerr A. and Sawada H. 2001. A revision of Rhizobium Frank 1889, with an amended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al., 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. Int. J. Syst. Evol. Microbiol. 51: 89-103.