Harnessing natural transformation in Actinobacillus pleuropneumoniae: A simple method for allelic replacements

FEMS Microbiology Letters

Volumn 233, Issue 2, 2004, Pages 277-281

  Bossé, Janine T ^a   Nash, John H E ^b   Simon Kroll, J ^a   Langford, Paul R ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Actinobacillus pleuropneumoniae; Allele replacement; Competence; Knockout; Transformation

Indexed keywords

KANAMYCIN; PLASMID DNA;

ACTINOBACILLUS PLEUROPNEUMONIAE; ALLELE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL GROWTH; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM COMPETENCE; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; GENE CASSETTE; GENE INSERTION; GROWTH CURVE; HAEMOPHILUS INFLUENZAE; NONHUMAN; PRIORITY JOURNAL; SEROTYPE; SODC GENE;

ACTINOBACILLUS PLEUROPNEUMONIAE; ALLELES; CULTURE MEDIA; MUTAGENESIS; PLASMIDS; TRANSFORMATION, GENETIC;

ACTINOBACILLUS; ACTINOBACILLUS PLEUROPNEUMONIAE; BACTERIA (MICROORGANISMS); HAEMOPHILUS; HAEMOPHILUS INFLUENZAE;

EID: 1842582680     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.02.022     Document Type: Article

References (18)

1. Bossé J.T., Janson H., Sheehan B.J., Beddek A.J., Rycroft A.N., Kroll J.S., Langford P.R. Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes Infect. 4:2002;225-235.
2. Fuller T.E., Martin S., Teel J.F., Alaniz G.R., Kennedy M.J., Lowery D.E. Identification of Actinobacillus pleuropneumoniae virulence genes using signature-tagged mutagenesis in a swine infection model. Microb. Pathog. 29:2000;39-51.
3. Sheehan B.J., Bossé J.T., Beddek A.J., Rycroft A.N., Kroll J.S., Langford P.R. Identification of Actinobacillus pleuropneumoniae genes important for survival during infection in its natural host. Infect. Immun. 71:2003;3960-3970.
4. Jansen R., Briaire J., Smith H.E., Dom P., Haesebrouck F., Kamp E.M., Gielkens A.L., Smits M.A. Knockout mutants of Actinobacillus pleuropneumoniae serotype 1 that are devoid of RTX toxins do not activate or kill porcine neutrophils. Infect. Immun. 63:1995;27-37.
5. Mulks M.H., Buysse J.M. A targeted mutagenesis system for Actinobacillus pleuropneumoniae. Gene. 165:1995;61-66.
6. Oswald W., Tonpitak W., Ohrt G., Gerlach G. A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker. FEMS Microbiol. Lett. 179:1999;153-160.
7. Sheehan B.J., Langford P.R., Rycroft A.N., Kroll J.S. [Cu,Zn]-Superoxide dismutase mutants of the swine pathogen Actinobacillus pleuropneumoniae are unattenuated in infections of the natural host. Infect. Immun. 68:2000;4778-4781.
8. Ward C.K., Lawrence M.L., Veit H.P., Inzana T.J. Cloning and mutagenesis of a serotype-specific DNA region involved in encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: concomitant expression of serotype 5a and 1 capsular polysaccharides in recombinant A. pleuropneumoniae serotype 1. Infect. Immun. 66:1998;3326-3336.
9. Fuller T.E., Thacker B.J., Mulks M.H. A riboflavin auxotroph of Actinobacillus pleuropneumoniae is attenuated in swine. Infect. Immun. 64:1996;4659-4664.
10. Dubnau D. DNA uptake in bacteria. Annu. Rev. Microbiol. 53:1999;217-244.
11. Williams P.M., Bannister L.A., Redfield R.J. The Haemophilus influenzae. sxy -1 mutation is in a newly identified gene essential for competence J. Bacteriol. 176:1994;6789-6794.
12. Chandler M.S. The gene encoding cAMP receptor protein is required for competence development in Haemophilus influenzae Rd. Proc. Natl. Acad. Sci. USA. 89:1992;1626-1630.
13. Dorocicz I.R., Williams P.M., Redfield R.J. The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence. J. Bacteriol. 175:1993;7142-7149.
14. ′ cyclic AMP (cAMP) phosphodiesterase modulates cAMP levels and optimizes competence in Haemophilus influenzae Rd J. Bacteriol. 180:1998;4401-4405.
15. Macfadyen L.P. Regulation of competence development in Haemophilus influenzae. J. Theor. Biol. 207:2000;349-359.
16. Wang Y., Goodman S.D., Redfield R.J., Chen C. Natural transformation and DNA uptake signal sequences in Actinobacillus actinomycetemcomitans. J. Bacteriol. 184:2002;3442-3449.
17. Garside L.H., Collins M., Langford P.R., Rycroft A.N. Actinobacillus pleuropneumoniae serotype 1 carrying the defined. aroA mutation is fully avirulent in the pig Res. Vet. Sci. 72:2002;163-167.
18. Herriott R.M., Meyer E.M., Vogt M. Defined nongrowth media for stage II development of competence in Haemophilus influenzae. J. Bacteriol. 101:1970;517-524.