Trp2063 and Trp2064 in the Factor Va C2 Domain Are Required for High-Affinity Binding to Phospholipid Membranes but Not for Assembly of the Prothrombinase Complex

Biochemistry

Volumn 43, Issue 14, 2004, Pages 4385-4393

  Peng, Weimin ^a   Quinn Allen, Mary Ann ^a   Kim, Suhng Wook ^a   Alexander, Kenneth A ^a   Kane, William H ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; ENERGY TRANSFER; ENZYMES; SOLVENTS; SURFACE PLASMON RESONANCE;

HIGH AFFINITY BINDING; MUTANT FACTORS;

BIOCHEMISTRY;

BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 5A; MUTANT PROTEIN; PHOSPHATIDYLSERINE; TRYPTOPHAN; ALANINE; PHOSPHOLIPID; RECOMBINANT PROTEIN; THROMBOPLASTIN;

ARTICLE; BILAYER MEMBRANE; BINDING AFFINITY; BINDING SITE; BLOOD CLOTTING FACTOR 5A C2 DOMAIN; ENZYME ACTIVITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROPHOBICITY; MEMBRANE BINDING; MOLECULAR STABILITY; PHOSPHOLIPID MEMBRANE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN INTERACTION; REGULATORY MECHANISM; SURFACE PLASMON RESONANCE; AMINO ACID SUBSTITUTION; ANIMAL; CATTLE; CELL LINE; CELL STRAIN COS1; CHEMISTRY; COMPARATIVE STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LIPID BILAYER; METABOLISM; MOTH; PROTEIN BINDING; PROTEIN PROCESSING; PROTEIN TERTIARY STRUCTURE;

INSERTION SEQUENCES;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; CATTLE; CELL LINE; COS CELLS; FACTOR VA; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; LIPID BILAYERS; MOTHS; PHOSPHATIDYLSERINES; PHOSPHOLIPIDS; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE; THROMBOPLASTIN; TRYPTOPHAN;

EID: 1842426740     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035763o     Document Type: Article

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