Mutations in a potential phospholipid binding loop in the C2 domain of factor V affecting the assembly of the prothrombinase complex

Blood Coagulation and Fibrinolysis

Volumn 11, Issue 1, 2000, Pages 89-100

  Nicolaes, G A F ^a   Villoutreix, B O ^b   Dahlback B ^b  

^a University Hospital Malmö   (Sweden)

^b NONE

Author keywords

APC; Coagulation; Factor V; Prothrombinase; Structural prediction; Threading; Thrombosis

Indexed keywords

BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 5; BLOOD CLOTTING FACTOR 8A; MONOCLONAL ANTIBODY; PHOSPHOLIPID;

ANIMAL CELL; ARTICLE; BLOOD CLOTTING; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; HUMAN; MEMBRANE BINDING; MOLECULAR MODEL; NONHUMAN; PHOSPHOLIPID MEMBRANE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION; THROMBOSIS;

EID: 0033953116     PISSN: 09575235     EISSN: None     Source Type: Journal    
DOI: 10.1097/00001721-200001000-00010     Document Type: Article

References (68)

1. 1. Jenny RJ, Pittman DD, Toole JT, Kriz RW, Aldape RA, Hewick MH, et al. Complete cDNA and derived amino acid sequence of human factor V. Proc Natl Acad Sci USA 1987; 84: 4846-4850.
2. 2. Dahlbäck B. Human coagulation factor V purification and thrombin-catalysed activation. J Clin Invest 1980; 66: 583-591.
3. 3. Kane WH, Majerus PW. Purification and characterisation of human coagulation factor V. J Biol Chem 1981; 256: 1002-1007.
4. 4. Suzuki K, Dahlbäck B, Stenflo J. Thrombin-catalysed activation of human coagulation factor V. J Biol Chem 1982; 257: 6556-6564.
5. 5. Monkovic D, Tracy P. Activation of human factor V by factor Xa and thrombin. Biochemistry 1990; 29: 1118-1128.
6. 6. Tans G, Nicolaes GAF, Thomassen MCLGD, Hemker HC, Van Zonneveld A-J, Pannekoek H, et al. Activation of human factor V by meizothrombin. J Biol Chem 1994; 269: 15969-15972.
7. 7. Esmon CT. J Biol Chem 1979; 254: 964-973.
8. 8. Guinto ER, Esmon CT. Formation of a calcium-binding site on bovine activated factor V following recombination of the isolated subunits. J Biol Chem 1982; 257: 10038-10043.
9. 9. Nesheim ME, Taswell JB, Mann KG. The contribution of bovine factor V and factor Va to the activity of the prothrombinase. J Biol Chem 1979; 254: 10952-10962.
10. 10. Rosing J, Tans G, Govers-Riemslag JWP, Zwaal RFA, Hemker HC. The role of phospholipids and factor Va in the prothrombinase complex. J Biol Chem 1980; 255: 274-283.
11. 11. Kalafatis M, Rand MD, Mann KG. Factor Va-membrane interaction is mediated by two regions located on the light chain of the cofactor. Biochemistry 1994; 33: 486-493.
12. 12. Lecompte MF, Bouix G, Mann KG. Electrostatic and hydrophobic interactions are involved in factor Va binding to membranes containing acidic phospholipids. J Biol Chem 1994; 269: 1905-1910.
13. 13. Govers-Riemslag JWP, Janssen MP, Zwaal RFA, Rosing J. Prothrombin activation on dioleoylphosphatidylcholine membranes. Eur J Biochem 1994; 220: 131-138.
14. 14. Cutsforth GA, Koppaka V, Krishnaswamy S, Wu JR, Mann KG, Lentz BR. Insights into the complex association of bovine factor V(a) with acidic-lipid-containing synthetic membranes. Biophys J 1996; 70(6): 2938-2949.
15. 15. Bloom JW, Nesheim ME, Mann KG. Phospholipid-binding properties of bovine factor V and factor Va. Biochemistry 1979; 18: 4419-4425.
16. 16. Higgins DL, Mann KG. The interaction of bovine factor V and factor V-derived peptides with phospholipid vesicles. J Biol Chem 1983; 258: 6503-6508.
17. 17. Van de Waart P, Bruls H, Hemker HC. Interaction of bovine blood clotting factor Va and its subunits with phospholipidvesicles. Biochemistry 1983;22:2427-2432.
18. 18. Pusey ML, Nelsestuen GL. Membrane binding properties of blood coagulation Factor V and derived peptides. Biochemistry 1984; 23: 6202-6210.
19. 19. Lindhout T, Govers Riemslag JW, van de Waart P, Hemker HC, Rosing, J. Factor Va-factor Xa interaction. Effects of phospholipid vesicles of varying composition. Biochemistry 1982; 21: 5494-5502.
20. 20. Stoylova S, Mann KG, Brisson A. Structure of membrane-bound human factor Va. FEBS Lett 1994; 351: 330-334.
21. 21. Ortel TL, Devore Carter D, Quinn Allen M, Kane WH. Deletion analysis of recombinant human factor V. Evidence for a phosphatidylserine binding site in the second C-type domain. J Biol Chem 1992; 267: 4189-4198.
22. 22. Tracy PB, Mann KG. Prothrombinase complex assembly on the platelet surface is mediated through the 74,000-dalton component of factor Va. Proc Natl Acad Sci USA 1983; 80: 2380-2384.
23. 23. van de Waart P, Hemker HC, Lindhout T. Interaction of prothrombin with factor Va-phospholipid complexes. Biochemistry 1984; 23: 2838-2842.
24. 24. Krishnaswamy S, Mann KG. The binding of factor Va to phospholipid vesicles. J Biol Chem 1988; 263: 5714-5723.
25. 25. Ortel TL, Quinn-Allen MA, Keller FG, Peterson JA, Larocca D, Kane WH. Localization of functionally important epitopes within the second C-type domain of coagulation factor V using recombinant chimeras. J Biol Chem 1994; 269: 15898-15905.
26. 26. Ortel TL. Clinical and laboratory manifestations ofanti-factor V antibodies. J Lab Clin Med 1999; 133: 326-334.
27. 27. Rosing J, Bakker HM, Thomassen MCLGD, Hemker HC, Tans G. Characterization of two forms of human factor Va with different cofactor activities. J Biol Chem 1993; 268: 21130-21136.
28. 28. Kim SW, Ortel TL, Quinn-Allen MA, Yoo L, Worfolf L, Zhai X, et al. Partial glycosylation at asparagine-2181 of the second C-type domain of human factor V modulates assembly of the prothrombinase complex. Biochemistry 1999; 38: 11448-11545.
29. 29. Nicolaes GAF, Villoutreix BO, Dahlbäck B. Partial glycosylation of Asn2181 in human factor V as a cause of molecular and functional heterogeneity, modulation of glycosylation efficiency by mutagenesis of the consensus sequence for N-linked glycosylation. Biochemistry 1999; 38: 13584-13591.
30. 30. Kane WH, Davie EW. Cloning of a cDNA coding for human factor V, a blood coagulation factor homologous to factor VIII and ceruloplasmin. Proc Natl Acad Sci USA 1986; 83: 6800-6804.
31. 31. Baumgartner S, Hofmann K, Chiquet Ehrismann R, Bucher P. The discoidin domain family revisited: new members from prokaryotes and a homology-based fold prediction. Protein Sci 1998; 7: 1626-1631.
32. 32. Villoutreix BO, Bucher P, Hofmann K, Baumgartner S, Dahlbäck B. Molecular models for the two discoidin domains of human blood coagulation factor V. J Mol Model 1998; 4: 268-275.
33. 33. Pellequer JL, Gale AJ, Griffin JH, Getzoff ED. Homology models of the C domains of blood coagulation factors V and VIII: a proposed membrane binding mode for FV and FVIII C2 domains. Blood Cell Mol Dis 1998; 24: 448-461
34. 34. Ito N, Phillips SE, Yadav KD, Knowles PF. Crystal structure of a free radical enzyme, galactose oxidase. J Mol Biol 1994; 238: 794-814.
35. 35. Suzuki K, Stenflo J, Dahlbäck B, Teodorsson B. Inactivation of human coagulation factor V by activated protein C. J Biol Chem 1983; 258: 1914-1920.
36. 36. Nicolaes GAF, Tans G, Thomassen MCLGD, Hemker HC, Pabinger I, Varadi K, et al. Peptide bond cleavages and loss of functional activity during inactivation of factor Va and factor VaR506Q by activated protein. J Biol Chem 1995; 270: 21158-21166.
37. 37. Thorelli E, Kaufman RJ, Dahlbäck B. Cleavage requirements for activation of factor V by factor Xa. Eur J Biochem 1997; 247: 12-20.
38. 38. Chase T Jr, Shaw E. Comparison of the esterase activities of trypsin, plasmin, and thrombin on guanidinobenzoate esters. Titration of the enzymes. Biochemistry 1969; 8: 2212-2224.
39. 39. Smith RL. Titration of activated bovine Factor X. J Biol Chem 1973; 248: 2418-2423.
40. 40. Kaufman RJ. Vectors used for expression in mammalian cells. Methods Enzymol 1990: 185: 487-511.
41. 41. Thorelli E, Kaufman RJ, Dahlbäck B. The C-terminal region of the factor V B-domain is crucial for the anticoagulant activity of factor V. J Biol Chem 1998; 273: 16140-16145.
42. 42. Bollag DM, Rozycki MD, Edelstein SJ. Protein methods. New York: Wiley-Liss Inc.; 1996.
43. 43. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970; 227: 680-685.
44. 44. Blake MS, Johnston KH, Russell Jones GJ, Gotschlich EC. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal Biochem 1984; 136: 175-179.
45. 45. Kim SW, Quinn-Allen MA, Camp T, Macedo-Ribeiro S, Fuentes-Prior P, Bode W, et al. Identification of a phosphatidylserine binding site within the C2 domain of human factor V using alanine scanning mutagenesis [Abstract] Thrombosis Haemostasis 1999; Suppl. 234: 13.
46. 46. Pratt KP, Fujikawa K, Takeshima K, Davie EW, Stoddard BS. Crystal structure of the factor VIII C2 domain [Abstract] Thrombosis Haemostasis 1999; Suppl. 234: 234.
47. 47. Lampe PD, Pusey ML, Wei GJ, Nelsestuen GL. Electron microscopy and hydrodynamic properties of blood clotting factor V and activation fragments of factor V with phospholipid vesicles. J Biol Chem 1984; 259: 9959-9964.
48. 48. Xue J, Kalafatis M, Silveira JR, Kung C, Mann KG. Determination of the disulphide bridges in factor Va heavy chain. Biochemistry 1994; 33: 13109-13116.
49. m in the prothrombinase reaction. Biochem Biopbys Res Commun 1983; 114: 526-532.
50. 50. Reithmeier RA. Characterization and modeling of membrane proteins using sequence analysis. Curr Opin Struct Biol 1995; 5: 491-500.
51. 51. Koshi JM, Bruno WJ. Major structural determinants of transmembrane proteins identified by principal component analysis. Proteins 1999; 34: 333-340.
52. 52. Pautsch A, Schulz GE. Structure of the outer membrane protein A transmembrane domain. Nat Struct Biol 1998; 5: 1013-1017.
53. 53. Yau WM, Wimley WC, Gawrisch K, White SH. The preference of tryptophan for membrane interfaces. Biochemistry 1998; 37: 14713-14718.
54. 54. Dougherty DA. Cation-pi interactions in chemistry and biology: a new view of benzene, Phe, Tyr, and Trp. Science 1996; 271: 163-168.
55. 55. Nozaki Y, Tanford C. The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Establishment of a hydrophobicity scale. J Biol Chem 1971; 246: 2211-2217.
56. 56. Levitt M. A simplified representation of protein conformations for rapid simulation of protein folding. J Mol Biol 1976; 104: 59-107.
57. 57. Billy D, Willems GM, Hemker HC, Lindhout T. Prothrombin contributes to the assembly of the factorVa-factor Xa complex at phospbatidylserine-containing phospholipid membranes. J Biol Chem 1995; 270: 26883-26889.
58. 58. Esmon CT, Jackson CM. The conversion of prothrombin to thrombin. IV. The function of the fragment 2 region during activation in the presence of factor V. J Biol Chem 1974; 249: 7791-7797.
59. 59. Kalafatis M, Rand MD, Mann KG. The mechanism of inactivation of human factor V and human factor Va by activated protein C. J Biol Chem 1994; 269: 31869-31880.
60. 60. Villoutreix BO, Dahlbäck B. Structural investigation of the A domains of human blood coagulation factor V by molecular modeling. Protein Sci 1998; 7: 1317-1325.
61. 61. Yegneswaran S, Wood GM, Esmon CT, Johnson AE. Protein S alters the active site location of activated protein C above the membrane surface. A fluorescence resonance energy transfer study of topography. J Biol Chem 1997; 272: 25013-25021.
62. 62. Husten EJ, Esmon CT, Johnson AE. The active site of blood coagulation factor Xa. Its distance from the phospholipid surface and its conformational sensitivity to components of the prothrombinase complex. J Biol Chem 1987; 262: 12953-12961.
63. 63. Stoylova SS, Lenting PJ, Kemball-Cook G, Holzenburg A. Electron crystallography of human blood coagulation factor VIII bound to phospholipid monolayers. J Biol Chem 1999; (in press).
64. 64. Pusey ML, Mayer LD, Wei GJ, Bloomfield VA, Nelsestuen GL. Kinetic and hydrodynamic analysis of blood clotting factor V-membrane binding. Biochemistry 1982; 21: 5262-5269.
65. 65. Guinto ER, Esmon CT. Loss of prothrombin and of factor Xa-factor Va interactions upon inactivation of factor Va by activated protein C. J Biol Chem 1984; 259: 13986-13992.
66. 66. Luckow EA, Lyons DA, Ridgeway TM, Esmon CT, Laue TM. Interaction of clotting factor V heavy chain with prothrombin and prethrombin 1 and role of activated protein C in regulating this interaction: analysis by analytical ultracentrifugation. Biochemistry 1989; 28: 2348-2354.
67. 67. Pryzdial ELG, Mann KG, The association of coagulation factor Xa and factor Va. J Cell Biochem 1991; 266: 8960-8977.
68. 68. Kalafatis M, Xue J, Lawler CM, Mann KG. Contribution of the heavy and light chains of factor Va to the interaction with factor Xa. Biochemistry 1994; 33: 6538-6545.