Extraction, purification and physical-chemical characterization of recombinant green fluorescent protein (GFPuv) expressed in Escherichia coli cells;Extração, purificação e caracterizaç ão físico-química da proteína verde fluorescente recombinante (GFPuv) expressa em Escherichia coli

Revista Brasileira de Ciencias Farmaceuticas/Brazilian Journal of Pharmaceutical Sciences

Volumn 39, Issue 4, 2003, Pages 457-466

  Chiarini, Eb ^a,b   Vessoni Penna, Thereza Christina ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b NONE   (Brazil)

Author keywords

Escherichia coli; GFPuv; Protein extraction, Three phase partitioning TPP; Recombinant green fluorescent protein

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYDROCHLORIC ACID; MERCAPTOETHANOL; RECOMBINANT PROTEIN;

ARTICLE; CHEMICAL ANALYSIS; CHROMATOGRAPHY; ESCHERICHIA COLI; EXTRACTION; FREEZING; HYDROPHOBICITY; NONHUMAN; PH; PHASE PARTITIONING; PHYSICAL CHEMISTRY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; SOLUBILITY; STANDARD; VIBRATION;

EID: 1842421329     PISSN: 15169332     EISSN: None     Source Type: Journal    
DOI: 10.1590/S1516-93322003000400014     Document Type: Article

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