Real-time quantitative polymerase chain reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA levels in Caco-2 cell lines, human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarclnomas

Drug Metabolism and Disposition

Volumn 30, Issue 1, 2002, Pages 4-6

  Nakamura, Tsutomu ^a   Sakaeda, Toshiyuki ^a   Ohmoto, Nobuko ^a   Tamura, Takao ^a   Aoyama, Nobuo ^a   Shirakawa, Toshiro ^a   Kamigaki, Takashi ^a   Nakamura, Takeshi ^a   Kim, Keih ^b   Kim, Soo Ryang ^b   Kuroda, Yoshikazu ^a   Matsuo, Masafumi ^a   Kasuga, Masato ^a   Okumura, Katsuhiko ^a  

^a KOBE UNIVERSITY   (Japan)

^b KOBE ASAHI HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450 3A; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCOPROTEIN P; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; MULTIDRUG RESISTANCE PROTEIN 2; UNCLASSIFIED DRUG;

ADENOCARCINOMA; ARTICLE; CELL STRAIN CACO 2; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADENOCARCINOMA; ADULT; AGED; AGED, 80 AND OVER; ARYL HYDROCARBON HYDROXYLASES; CACO-2 CELLS; COLON; COLORECTAL NEOPLASMS; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 ENZYME SYSTEM; DUODENUM; ENTEROCYTES; FEMALE; GENE EXPRESSION; GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (NADP+); HUMANS; INTESTINAL MUCOSA; MALE; MEMBRANE TRANSPORT PROTEINS; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; OXIDOREDUCTASES, N-DEMETHYLATING; P-GLYCOPROTEIN; POLYMERASE CHAIN REACTION; RECTUM; RNA, MESSENGER;

STAPHYLOCOCCUS PHAGE 3A;

EID: 18244390013     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: 10.1124/dmd.30.1.4     Document Type: Article

References (16)

1. Intestinal drug absorption and metabolism in cell cultures: Caco-2 and beyond
2. Intestinal cell models: Their use in evaluating the metabolism and absorption of xenobiotics
3. Technological advances in high-throughput screening
4. A novel method for real time quantitative PT-PCR
5. Real time quantitative PCR
6. Carrier-mediated transport and efflux mechanisms in Caco-2 cells
7. Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2)
8. htert expression correlates with MYC over-expression in human prostate cancer
9. Conditionally immortalized intestinal epithelial cells
10. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture
11. Preclinical drug metabolism in the age of high-throughput screening: An industrial perspective
12. Expression of enzymatically active CYP3A4 by Caco-2 cells grown on extracellular matrixcoated permeable supports in the presence of lα,25-dihydroxyvitamin D3
13. High-throughput approaches for evaluating absorption, distribution, metabolism and excretion properties of lead compounds
14. Transcellular transport of benzoic acid across Caco-2 cells by a pH-dependent and carrier-mediated transport mechanism
15. The barrier function of CYP3A4 and P-glycoprotein in the small bowel
16. Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system