A simple method of detecting K-ras point mutations in stool samples for colorectal cancer screening using one-step polymerase chain reaction/restriction fragment length polymorphism analysis

Clinica Chimica Acta

Volumn 318, Issue 1-2, 2002, Pages 107-112

  Nishikawa, Takashi ^a,c   Maemura, Kentaro ^a   Hirata, Ichiro ^a   Matsuse, Ryouichi ^b   Morikawa, Hiroshi ^a   Toshina, Ken ^a   Murano, Mitsuyuki ^a   Hashimoto, Keiichi ^a   Nakagawa, Yoshihito ^a   Saitoh, Osamu ^a   Uchida, Kazuo ^b   Katsu, Kenichi ^a  

^a OSAKA MEDICAL COLLEGE   (Japan)

^b Kyoto Medical Science Laboratory   (Japan)

^c NONE   (United States)

Author keywords

Colorectal cancer; K ras; PCR RFLP; Stool

Indexed keywords

SYNTHETIC DNA;

ADULT; AGED; ANALYTIC METHOD; ARTICLE; BASE PAIRING; CANCER DIAGNOSIS; CANCER PATIENT; CANCER SCREENING; CELL STRAIN HT29; CLINICAL ARTICLE; CODON; COLORECTAL CARCINOMA; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA EXTRACTION; EXON; FECES ANALYSIS; FEMALE; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE MUTATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; MASS SCREENING; ONCOGENE K RAS; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SILVER STAINING; WILD TYPE;

ADULT; AGED; AGED, 80 AND OVER; COLORECTAL NEOPLASMS; DNA; DNA PRIMERS; FECES; FEMALE; GENES, RAS; HUMANS; MALE; MIDDLE AGED; POINT MUTATION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TUMOR CELLS, CULTURED;

EID: 18244369274     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(01)00806-3     Document Type: Article

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