Immunohistochemical detection of HCV in cirrhosis, dysplastic nodules, and hepatocellular carcinomas with parallel-tissue quantitative RT-PCR

Modern Pathology

Volumn 14, Issue 5, 2001, Pages 496-505

  Rullier, Anne ^a,b   Trimoulet, Pascale ^a   Urbaniak, Rosa ^b   Winnock, Maria ^b   Zauli, Daniela ^c   Ballardini, Giorgio ^c   Rosenbaum, Jean ^b   Balabaud, Charles ^b   Bioulac Sage, Paulette ^a,b   Le Bail, Brigitte ^a,b  

^a HÔPITAL PELLEGRIN   (France)

^b UNIVERSITÉ DE BORDEAUX   (France)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Dysplastic nodules; HCV; Hepatocellular carcinoma; Immunohistochemistry; In situ detection; Liver neoplasms; Quantitative RT PCR; Viral carcinogenesis

Indexed keywords

VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CANCER RISK; CELL DIFFERENTIATION; CELL PROLIFERATION; HEPATITIS C VIRUS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LIVER CARCINOGENESIS; LIVER CELL CARCINOMA; LIVER CIRRHOSIS; LIVER NODULE; MAJOR CLINICAL STUDY; MALIGNANT TRANSFORMATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION;

CARCINOMA, HEPATOCELLULAR; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FOCAL NODULAR HYPERPLASIA; HEPACIVIRUS; HEPATITIS C; HUMANS; IMMUNOHISTOCHEMISTRY; LIVER CIRRHOSIS; LIVER NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; VIRAL CORE PROTEINS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 17744391965     PISSN: 08933952     EISSN: None     Source Type: Journal    
DOI: 10.1038/modpathol.3880338     Document Type: Article

References (51)

1. Bannasch P. Pathogenesis of hepatocellular carcinoma: sequential cellular, molecular and metabolic changes. Prog Liver Dis 1996;14:161-97.
2. International Working Party. Terminology of nodular hepatocellular lesions. Hepatology 1995;22:903-93.
3. Di Bisceglie AM. Hepatitis C and hepatocellular carcinoma. Hepatology 1997;26(1 Suppl):34S-8S.
4. Colombo M, Fasani P, Romeo R. Hepatitis C virus and hepatocellular carcinoma. Res Virol 1997;148:127-34.
5. Le Bail B, Belleannée G, Bernard PH, Saric J, Balabaud C, Bioulac-Sage P. Adenomatous hyperplasia in cirrhotic livers: histological evaluation, cellular density, and proliferative activity of 35 macronodular lesions in the cirrhotic explants of 10 adult French patients. Hum Pathol 1995;26:897-906.
6. Kew MC. Hepatocellular carcinoma with and without cirrhosis. A comparison in southern African blacks. Gastroenterology 1989;97:136-9.
7. De Mitri M, Poussin K, Baccarini P, Pontisso P, D'Errico A, Simon N, et al. HCV-associated liver cancer without cirrhosis. Lancet 1995;345:413-5.
8. Bréchot C. The direct interplay between HCV NS5A protein and interferon transduction signal: from clinical to basic science. J Hepatol 1999;30:1152-4.
9. Komminoth P, Adams V, Long AA, Roth J, Saremaslani P, Flury R, et al. Evaluation of methods for hepatitis C virus detection in archival liver biopsies. Comparison of histology, immunohistochemistry, in situ hybridization, reverse transcriptase polymerase chain reaction (RT-PCR) and in situ RT-PCR. Pathol Res Pract 1994;190:1017-25.
10. Negro F, Hadengue A. Détection de l'ARN du virus de l'hépatite C par hybridation in situ: une analyse critique. Gastroenterol Clin Biol 1997;21:93-7.
11. Blight K, Rowland R, Hall P, Lesniewski RR, Trowbridge R, LaBrooy JT, et al. Immunohistochemical detection of the NS4 antigen of hepatitis C virus and its relation to histopathology. Am J Pathol 1993;143:1568-73.
12. Chamlian A, Benkoël L, Sahel J, Cherid A, Brisse J, Ikoli JF, et al. Immunohistochemical detection of hepatitis C related c100-3 and core antigens in formalin-fixed liver tissue. Cell Mol Biol 1996;42:557-66.
13. Gonzales Peralta RP, Fang JWS, Davis GL, Gish R, Tsukiyama-Kohara K, Kohara M, et al. Optimization for detection of hepatitis C virus antigen in the liver. J Hepatol 1994;20:143-7.
14. Hiramatsu N, Hayashi N, Haruna Y, Kasahara A, Fusamoto H, Mori C, et al. Immunohistochemical detection of hepatitis C infected hepatocytes in chronic liver disease with monoclonal antibody to core, envelope and NS3 regions of the hepatitis C virus genome. Hepatology 1994;20:143-7.
15. Krawczynski K, Beach MJ, Bradley D, Kuo G, Di Bisceglie AM, Houghton M, et al Hepatitis C virus antigen in hepatocytes: immunomorphologic detection and identification. Gastroenterology 1992;103:622-9.
16. Nouri-Aria KT, Sallie R, Mizokami M, Portmann BC, Williams R. Intrahepatic expression of hepatitis C virus antigens in chronic liver disease. J Pathol 1995;175:77-83.
17. Nuovo GJ, Lidonici K, MacConnell P, Lane B. Intracellular localization of polymerase chain reaction (PCR)-amplified hepatitis C cDNA. Am J Surg Pathol 1995;175:77-83.
18. Tang L, Tanaka Y, Enomoto N, Marumo F, Sato C. Detection of hepatitis C virus RNA in hepatocellular carcinoma by in situ hybridization. Cancer 1995;76:2211-6.
19. Uchida T, Shikata T, Tanaka E, Kiyosawa K. Immunoperoxidase staining of hepatitis C virus in formalin-fixed, paraffin-embedded needle liver biopsies. Virchows Arch 1994;424: 465-9.
20. Sansonno D, Cornacchiulo V, Racanelli V, Damacco F. In situ simultaneous detection of hepatitis C virus RNA and hepatitis C virus-related antigens in hepatocellular carcinoma. Cancer 1997;80:22-33.
21. Uchida T, Shikata T. Hepatitis C virus appears to replicate not only in hepatocytes but also in hepatocellular carcinoma cells as demonstrated by immunostaining. Pathol Int 1994; 44:832-6.
22. Bedossa P, Poynard T, the METAVIR Cooperative Study Group. An algorithm for the grading of activity in chronic hepatitis C. Hepatology 1996;24:289-93.
23. Edmondson HA, Steiner PE. Primary carcinoma of the liver: a study of 100 cases among 48900 necropsies. Cancer 1954; 7:462-503.
24. Ballardini G, Groff P, Giostra F, Francesconi R, Miniero R, Ghetti S, et al. Hepatocellular codistribution of c100, c33, c22 and NS5 hepatitis C virus antigens detected by using immunopurified polyclonal spontaneous human antibodies. Hepatology 1995;21:730-4.
25. Erali M, Ashwood ER, Hillyard DR. Performance characteristics of the COBAS AMPLICOR hepatitis C virus MONITOR test, version 2.0. Am J Clin Pathol 2000;114:180-7.
26. Garrigue I, Pellegrin I, Hoen B, Dumon B, Harzic M, Schrive MH, et al. Cell-associated HIV-1-DNA quantitation after highly active antiretroviral therapy-treated primary infection in patients with persistently undetectable plasma HIV-1 RNA. AIDS 2000;14:2851-5.
27. Martin J, Navas S, Quiroga JA, Colucci G, Pardo M, Carreno V. Quantitation of hepatitis C virus in liver and peripheral blood mononuclear cells from patients with chronic hepatitis C virus infection. J Med Virol 1998;54:265-70.
28. Duvoux C, Pawlowsky JM, Bastie A, Cherqui D, Soussy CJ, Dhumeaux D. Low replication levels in end-stage hepatitis C-virus-related liver disease. J Hepatol 1999;31:593-7.
29. Takeda S, Shibata M, Morishima T, Harada A, Nakao A, Takagi H, et al. Hepatitis C virus infection in hepatocellular carcinoma. Cancer 1992;70:2255-9.
30. Gerber MA. Relation of hepatitis C virus and hepatocellular carcinoma. J Hepatol 1993;17:5108-11.
31. Saito K, Sullivan D, Haruna Y, Theise ND, Thung SN, Gerber MA. Detection of hepatitis C virus RNA sequences in hepatocellular carcinoma and its precursors by microdissection polymerase chain reaction. Arch Pathol Lab Med 1997;121: 400-3
32. Kobayashi S, Hayashi H, Itoh Y, Asano T, Isono K. Detection of minus-strand hepatitis C virus RNA in tumor tissues of hepatocellular carcinoma. Cancer 1994;73:48-52.
33. Tong CY, Hollingsworth RC, Williams H, Irving WL, Gilmore IT. Effect of genotypes on the quantification of hepatitis C virus (HCV) RNA in clinical samples using the Amplicor HCV Monitor Test and the Quantiplex HCV RNA 2.0 Assay (bDNA). J Med Virol 1998;55:191-6.
34. Lunel F, Cresta P, Vitour D, Payan C, Dumont B, Frangeul L, et al. Comparative evaluation of hepatitis C virus RNA quantitation by branched DNA, NASBA, and Monitor assays. Hepatology 1999;29:528-35.
35. Dries V, von Both I, Müller M, Gerken G, Schirmacher P, Odenthal M, et al. Detection of hepatitis C virus in paraffin-embedded liver biopsies of patients negative for viral RNA serum. Hepatology 1999;29:223-9.
36. Haruna Y, Hayashi N, Kamada T, Hytiroglou P, Thung SN, Gerber MA. Expression of hepatitis C virus in hepatocellular carcinoma. Cancer 1994;73:2253-8.
37. Paterlini P, Driss F, Nalpas B, Pisi E, Franco D, Berthelot P, et al. Persistence of hepatitis B and hepatitis C viral genomes in primary liver cancers from HBsAg-negative patients: a study of a low-endemic area. Hepatology 1993;17:20-9.
38. Sakamuro D, Furukawa T, Takegami T. Hepatitis C virus nonstructural protein NS3 transforms NIH3T3. J Virol 1995; 69:3893-6.
39. Gale M, Kwieciszewski B, Dossett M, Nakao H, Katze MG. Antiapoptotic and oncogenic potentials of hepatitis C virus are linked to interferon resistance by viral repression of the PKR protein kinase. J Virol 1999;73:6506-16.
40. Zhu N, Khoshnan A, Schneider R, Matsumoto M, Dennert G, Ware C, et al. Hepatitis C virus core protein binds to the cytoplasmic domain of tumor necrosis factor (TNF) receptor 1 and enhances TNF-induced apoptosis. J Virol 1998;72: 3691-7.
41. You LR, Chen CM, Wu Lee YH. Hepatitis C virus core protein enhances NF-KB signal pathway triggering by lymphotoxin-B receptor ligand and tumor necrosis factor alpha. J Virol 1999;73:1672-81.
42. Shrivastava A, Manna SK, Ray R, Aggarwal BB. Ectopic expression of hepatitis C virus core protein differentially regulates nuclear transcription factors. J Virol 1998;72:9722-8.
43. Ruggieri A, Harada T, Matsuura Y, Miyamura T. Sensitization to Fas-mediated apoptosis by hepatitis C virus core protein. Virology 1997;229:68-76.
44. Ray RB, Mayer K, Steele R, Shrivastava A, Aggarwal BB, Ray R. Inhibition of tumor necrosis factor (TNF-alpha)-mediated apoptosis by hepatittis C virus core protein. J Biol Chem 1998;273:2256-9.
45. Marusawa H, Hijikata M, Chiba T, Shimotohono K. Hepatitis C virus core protein inhibits Fas- and tumor necrosis factor alpha-mediated apoptosis via NF-KB activation. J Virol 1999; 72:4713-20.
46. Moriya K, Fujie H, Shintani Y, Yotsuyanagi H, Tsutsumi T, Ishibashi K, et al. The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice. Nat Med 1998;4:1065-7.
47. Matsuura Y, Harada T, Makimura M, Sato M, Aizaki H, Suzuki T, et al. Characterization of HCV structural proteins expressed in various animal cells. Intervirology 1994; 37:114-8.
48. Suzuki R, Matsuura Y, Suzuki T, Ando A, Chiba J, Harada S, et al. Nuclear localization of the truncated core protein with its hydrophobic C terminus delated. J Gen Virol 1995;76: 53-61.
49. Barba G, Harper F, Harada T, Kohara M, Goulinet S, Matsuura Y, et al. Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc Natl Acad Sci USA 1997;94:1200-5.
50. Rüster B, Zuieem S, Roth WK. Hepatitis C virus sequences encoding truncated core proteins detected in hepatocellular. carcinoma. Biochem Biophys Res 1996;219:911-5.
51. Horie C, Iwanata H, Horie T, Shimizu I, Yoshimoto K, Yogita S, et al. Detection of different quasispecies of hepatitis C virus core region in cancerous and noncancerous lesions. Biochem Biophys Res 1996;218:674-81.