Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA

FEBS Letters

Volumn 579, Issue 11, 2005, Pages 2480-2484

  Dueckershoff, Katharina ^a   Unger, Matthias ^b   Frank, Andreas ^b   Gillam, Elizabeth M J ^c   Guengerich, F Peter ^d   Warzecha, Heribert ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c UNIVERSITY OF QUEENSLAND   (Australia)

^d VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cotinine; Cytochrome P450 2A6; Nicotiana tabacum; Nicotine metabolism; Secondary metabolism

Indexed keywords

ALKALOID; COMPLEMENTARY DNA; COTININE; CYTOCHROME P450; CYTOCHROME P450 2A6; NICOTINE; RECOMBINANT ENZYME;

ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE TRANSFER; GENETIC ANALYSIS; IN VIVO STUDY; MAMMAL; METABOLIC ENGINEERING; METABOLISM; METABOLITE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; RHIZOBIUM; TOBACCO; TRANSGENIC PLANT; WILD TYPE;

EID: 17644409032     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2005.02.082     Document Type: Article

References (29)

1. D. Werck-Reichhart, S. Bak, and S. Paquette Cytochromes P450 C.R. Somerville E.M. Meyerowitz The Arabidopsis Book 2002 American Society of Plant Biologists Rockville, MD Available from: http://dx.doi.org/10.1199/tab.0028
2. I. Werck-Reichhart, I. Hehn, and I. Didierjean Cytochromes P450 for engineering herbicide tolerance Trends Plant Sci. 5 2000 116 123
3. M.A. Schuler, and D. Werck-Reichhart Functional Genomics of P450s Annu. Rev. Plant Physiol. Plant Mol. Biol. 54 2003 629 667
4. C. Chapple Molecular-Genetic Analysis of Plant Cytochrome P450-Dependent Monooxygenases Annu. Rev. Plant Physiol. Plant Mol. Biol. 49 1998 311 343
5. N. Tijet, C. Helvig, F. Pinot, R. Le Bouquin, A. Lesot, F. Durst, J.P. Salaun, and I. Benveniste Functional expression in yeast and characterization of a clofibrate-inducible plant cytochrome P-450 (CYP94A1) involved in cutin monomers synthesis Biochem J. 332 1998 583 589
6. C.A. Helliwell, P.M. Chandler, A. Poole, E.S. Dennis, and W.J. Peacock The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway Proc. Natl. Acad. Sci. USA 98 2001 2065 2070
7. M. Mueller Enzymes involved in jasmonic acid biosynthesis Physiol. Plant 100 1997 653 663
8. M. Morant, S. Bak, B.L. Moller, and D. Werck-Reichhart Plant cytochromes P450: tools for pharmacology, plant protection and phytoremediation Curr. Opin. Biotechnol. 14 2003 151 162
9. M. Wink Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective Phytochemistry 64 2003 3 19
10. B. Siminszky, F.T. Corbin, E.R. Ward, T.J. Fleischmann, and R.E. Dewey Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides Proc. Natl. Acad. Sci. USA 96 1999 1750 1755
11. N. Shiota, A. Nagasawa, T. Sakaki, Y. Yabusaki, and H. Ohkawa Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome P4501A1 (CYP1A1) and yeast NADPH-cytochrome P450 oxidoreductase Plant Physiol. 106 1994 17 23
12. N. Shiota, S. Kodama, H. Inui, and H. Ohkawa Expression of human cytochromes P450 1A1 and P450 1A2 as fused enzymes with yeast NADPH-cytochrome P450 oxidoreductase in transgenic tobacco plants Biosci. Biotechnol. Biochem. 64 2000 2025 2033
13. J.M. Humphreys, and C. Chapple Molecular 'pharming' with plant P450s Trends Plant Sci. 5 2000 271 272
14. R.A. Dixon Natural products and plant disease resistance Nature 411 2001 843 847
15. K.M. Oksman-Caldentey, and D. Inze Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites Trends Plant Sci. 9 2004 433 440
16. K.A. Feldmann Cytochrome P450s as genes for crop improvement Curr. Opin. Plant Biol. 4 2001 162 167
17. W. Jung, O. Yu, S.M. Lau, D.P. O'Keefe, J. Odell, G. Fader, and B. McGonigle Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes Nat. Biotechnol. 18 2000 208 212
18. F.P. Guengerich Cytochrome P450 enzymes in the generation of commercial products Nat. Rev. Drug Discov. 1 2002 359 366
19. E.M. Gillam, and F.P. Guengerich Exploiting the versatility of human cytochrome P450 enzymes: the promise of blue roses from biotechnology IUBMB Life 52 2001 271 277
20. S.L. Doty, T.Q. Shang, A.M. Wilson, J. Tangen, A.D. Westergreen, L.A. Newman, S.E. Strand, and M.P. Gordon Enhanced metabolism of halogenated hydrocarbons in transgenic plants containing mammalian cytochrome P450 2E1 Proc. Natl. Acad. Sci. USA 97 2000 6287 6291
21. K. Saito, M. Noji, S. Ohmori, Y. Imai, and I. Murakoshi Integration and expression of a rabbit liver cytochrome P-450 gene in transgenic Nicotiana tabacum Proc. Natl. Acad. Sci. USA 88 1991 7041 7045
22. A. Le Gal, Y. Dreano, D. Lucas, and F. Berthou Diversity of selective environmental substrates for human cytochrome P450 2A6: alkoxyethers, nicotine, coumarin, N-nitrosodiethylamine, and N-nitrosobenzylmethylamine Toxicol. Lett. 144 2003 77 91
23. S.S. Hecht, J.B. Hochalter, P.W. Villalta, and S.E. Murphy 2′-Hydroxylation of nicotine by cytochrome P450 2A6 and human liver microsomes: formation of a lung carcinogen precursor Proc. Natl. Acad. Sci. USA 97 2000 12493 12497
24. J.C. Carrington, and D.D. Freed Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region J. Virol. 64 1990 1590 1597
25. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning - A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor
26. H. Chen, R.S. Nelson, and J.L. Sherwood Enhanced recovery of transformants of Agrobacterium tumefaciens after freeze-thaw transformation and drug selection Biotechniques 16 1994 664 668 670
27. P. Gallois, and P. Marinho Leaf disk transformation using Agrobacterium tumefaciens-expression of heterologous genes in tobacco Methods Mol. Biol. 49 1995 39 48
28. S.O. Rogers, and A.J. Bendich Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues Plant Mol. Biol. 5 1985 69 76
29. A. Rosco, H.H. Pauli, W. Priesner, and T.M. Kutchan Cloning and heterologous expression of NADPH-cytochrome P450 reductases from the Papaveraceae Arch. Biochem. Biophys. 348 1997 369 377