The CTB1 gene encoding a fungal polyketide synthase is required for cercosporin biosynthesis and fungal virulence of Cercospora nicotianae

Molecular Plant-Microbe Interactions

Volumn 18, Issue 5, 2005, Pages 468-476

  Choquer, Mathias ^a   Dekkers, Katherine L ^a   Chen, Hui Qin ^a   Cao, Lihua ^a   Ueng, Peter P ^b   Daub, Margaret E ^c   Chung, Kuang Ren ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Gene disruption; Pathogenicity; Perylenequinone

Indexed keywords

CERCOSPORIN; DRUG DERIVATIVE; MYCOTOXIN; PERYLENE; POLYKETIDE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; ASCOMYCETES; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PATHOGENICITY; PLANT LEAF; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; TOBACCO; VIRULENCE;

AMINO ACID SEQUENCE; ASCOMYCOTA; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; MYCOTOXINS; PERYLENE; PLANT LEAVES; POLYKETIDE SYNTHASES; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TOBACCO; VIRULENCE;

CERCOSPORA NICOTIANAE; ILEX COOKII; NICOTIANA TABACUM;

EID: 17644387189     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI-18-0468     Document Type: Article

References (44)

1. Altschul, S. F., Madden, T. L., Schafter, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Balis, C., and Payne, M. G. 1971. Triglycerides and cercosporin from Cercospora beticola: Fungal growth and cercosporin production. Phytopathology 61:1477-1484.
3. Brown, D. W., Yu, J.-H., Kelkar, H. S., Fernandes, M., Nesbitt, T. C., Keller, N. P., Adams, T. H., and Leonard, T. J. 1996. Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. Proc. Natl. Acad. Sci. U.S.A. 93:1418-1422.
4. Callahan, T. M., Rose, M. S., Meade, M. J., Ehrenshaft, M., and Upchurch, R. G. 1999. CFP, the putative cercosporin transporter of Cercospora kikuchii, is required for wild type cercosporin production, resistance, and virulence on soybean. Mol. Plant-Microbe Interact. 12:901-910.
5. Calpouzos, L., and Stalknecht, G. F. 1967. Symptoms of Cercospora leaf spot of sugar beets influenced by light intensity. Phytopathology 57:799-800.
6. Catlett, N. L., Lee, B.-N., Yoder, O. C., and Turgeon, B. G. 2003. Split-marker recombination for efficient targeted deletion of fungal genes. Fungal Genet. Newsl. 50:9-11.
7. Chung, K.-R. 2003. Involvement of calcium/calmodulin signaling in cercosporin toxin biosynthesis by Cercospora nicotianae. Appl. Environ. Microbiol. 69:1187-1196.
8. Chung, K.-R., Shilts, T., Li, W., and Timmer, L. W. 2002. Engineering a genetic transformation system for Colletotrichum acutatum, the causal fungus of lime anthracnose and postbloom fruit drop. FEMS (Fed. Eur. Microbiol. Soc.) Microbiol. Lett. 213:33-39.
9. Chung, K.-R., Daub, M. E., and Ehrenshaft, M. 2003a. Expression of cercosporin toxin resistance gene (CKG1) as a dicistronic unit with a functionally unrelated gene in the phytopathogenic fungus Cercospora nicotianae. Curr. Genet. 43:415-424.
10. Chung, K.-R., Daub, M. E., Kuchler, K., and Schüller, C. 2003b. The CRG1 gene required for resistance to the singlet oxygen-generating cercosporin toxin in Cercospora nicotianae encodes a putative fungal transcription factor. Biochem. Biophys. Res. Commun. 302:302-310.
11. Chung, K.-R., Ehrenshaft, M., Wetzel, D. K., and Daub, M. E. 2003c. Cercosporin toxin deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae. Mol. Genet. Genomics 270:103-113.
12. Daub, M. E. 1982a. Peroxidation of tobacco membrane lipids by the photosensitizing toxin, cercosporin. Plant Physiol. 69:1361-1364.
13. Daub, M. E. 1982h. Cercosporin, a photosensitizing toxin from Cercospora species. Phytopathology 72:370-374.
14. Daub, M. E., and Ehrenshaft, M. 2000. The photoactivated Cercospora toxin cercosporin: Contributions to plant disease and fundamental biology. Annu. Rev. Phytopathol. 38:461-490.
15. Daub, M. E., and Hangarter, R. P. 1983. Production of singlet oxygen and superoxide by the fungal toxin, cercosporin. Plant Physiol. 73:855-857.
16. Daub, M. E., Leisman, G. B., Clark, R. A., and Bowden, E. F. 1992. Reduced detoxification as a mechanism of fungal resistance to singlet-oxygen-generating photosensitizers. Proc. Natl. Acad. Sci. U.S.A. 89:9588-9592.
17. Daub, M. E., Li, M., Bilski, P., and Chignell, C. F. 2000. Dihydrocercosporin singlet oxygen production and subcellular localization: A possible defense against cercosporin phototoxicity in Cercospora. Photochem. Photobiol. 71:135-140.
18. Ehrenshaft, M., and Upchurch, R. G. 1991. Isolation of light-enhanced cDNA clones of Cercospora kikuchii. Appl. Environ. Microbiol. 57:2671-2676.
19. Ehrenshaft, M., and Upchurch, R. G. 1993. Host protein(s) induces accumulation of the toxin cercosporin and mRNA in a phytopathogenic strain of Cercospora kikuchii. Physiol. Plant Pathol. 79:157-164.
20. Ehrenshaft, M., Jenns, A. J., Chung, K.-R., and Daub, M. E. 1998. SOR1, a gene required for photosensitizer and singlet oxygen resistance in Cercospora fungi, is highly conserved in divergent organisms. Mol. Cell 1:603-609.
21. Ehrenshaft, M., Bilski, P., Li, M., Chignell, C. F., and Daub, M. E. 1999. A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 96:9374-9378.
22. Fairhead, C., Llorente, B., Denis, F., Soler, M., and Dujon, B. 1996. New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using 'split-marker' recombination. Yeast 12:1439-1457.
23. Fajola, A. O. 1978. Cercosporin, a phytotoxin from Cercospora species. Physiol. Plant Pathol. 13:157-164.
24. Feng, G. H., and Leonard, T. J. 1995. Characterization of the polyketide synthase gene (pksL1) required for aflatoxin biosynthesis in Aspergillus parasiticus. J. Bacteriol. 177:6246-6254.
25. Fore, S. A. Daub, M. E., and Beute, M. K. 1988. Phytotoxic substances produced by some isolates of Cercospora arachidicola are not cercosporin. Phytopathology 78:1082-1086.
26. Fujii, I., Mori, Y., Watanabe, A., Kubo, Y., Tsuji, G., and Ebizuka, Y. 2001. Identification of claisen cyclase domain in fungal polyketide synthase WA, a naphthopyrone synthase of Aspergillus nidulans. Chem. Biol. 8:189-197.
27. Jenns, A. E., and Daub, M. E. 1995. Characterization of mutants of Cercospora nicotianae sensitive to the toxin cercosporin. Phytopathology 85:906-912.
28. Jenns, A. E., Daub, M. E., and Upchurch, R. G. 1989. Regulation of cercosporin accumulation in culture by medium and temperature manipulation. Phytopathology 79:213-219.
29. Katz, L., and Donadio, S. 1993. Polyketide synthesis: Prospects for hybrid antibiotics. Annu. Rev. Microbiol. 47:875-912.
30. Keller, N. P., and Hohn, T. M. 1997. Metabolic pathway gene clusters in filamentous fungi. Fungal Genet. Biol. 21:17-29.
31. Kozak, M. 1984. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 12:857-872.
32. Kuyama, S., and Tamura, T. 1957. Cercosporin. A pigment of Cercospora kikuchii Matsumoto et Tomoyasu. I. Cultivation of fungus, isolation and purification of pigment. J. Am. Chem. Soc. 79:5725-5726.
33. Lu, S., Lyngholm, L., Yang, G., Bronson, C., Yoder, O. C., and Turgeon, B. G. 1994. Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration. Proc. Natl. Acad. Sci. U.S.A. 91:12649-12653.
34. Macri, F., and Vianello, A. 1979. Photodynamic activity of cercosporin on plant tissues. Plant Cell Environ. 2:267-271.
35. Mayorga, M. E., and Timberlake, W. E. 1992. The developmentally regulated Aspergillus nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases. Mol. Gen. Genet. 235:205-212.
36. Okubo, A., Yamazak, S., and Fuwa, K. 1975. Biosynthesis of cercosporin. Agric. Biol. Chem. 39:1173-1175.
37. Radford, A., and Parish, J. H. 1997. The genome and genes of Neurospora crassa. Fungal Genet. Biol. 21:258-266.
38. Sambrook, J., and Russell, D. W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY. U.S.A.
39. Shim, W.-B., and Dunkle, L. D. 2003. CZK3, a MAP kinase kinase kinase homolog in Cercospora zeae-maydis, regulates cercosporin biosynthesis, fungal development, and pathogenesis. Mol. Plant-Microbe Interact. 16:760-768.
40. Sweigard, J. A., Chumley, F. C., Carroll, A. M., Farrall, L., and Valent, B. 1997. A series of vectors for fungal transformation. Fungal Genet. Newsl. 44:52-53.
41. Tsai, H.-F., Chang, Y. C., Washburn, R. G., Wheeler, M. H., and Kwon-Chung, K. L. 1998. The developmentally regulated alb1 gene of Aspergillus fumigatus: Its role in modulation of conidial morphology and virulence. J. Bacteriol. 180:3031-3038.
42. Upchurch, R. G., Walker, D. C., Rollins, J. A., Ehrenshaft, M., and Daub, M. E. 1991. Mutants of Cercospora kikuchii altered in cereosporin synthesis and pathogenicity. Appl. Environ. Microbiol. 57:2940-2945.
43. Yamazaki, S., Okube, A., Akiyama, Y., and Fuwa, K. 1975. Cercosporin, a novel photodynamic pigment isolated from Cercospora kikuchii. Agric. Biol. Chem. 39:287-288.
44. Yang, G., Rose, M., Turgeon, B. G., and Yoder, O. C. 1996. A polyketide synthase is required for fungal virulence and production of the polyketide T-toxin. Plant Cell 8:2139-2150.