Quantitative real-time PCR detection of oral Enterococcus faecalis in humans

Archives of Oral Biology

Volumn 50, Issue 6, 2005, Pages 575-583

  Sedgley, C M ^a   Nagel, A C ^a   Shelburne, C E ^a   Clewell, D B ^a   Appelbe, O ^a   Molander, A ^b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

16S rRNA; Culturing; Enterococcus faecalis; Human; Oral; Quantitative real time PCR

Indexed keywords

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS;

EID: 17644369680     PISSN: 00039969     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.archoralbio.2004.10.017     Document Type: Article

References (30)

1. G.W. Tannock, and G. Cook Enterococci as members of the intestinal microflora of humans M.S. Gilmore The enterococci: pathogenesis, molecular biology, and antibiotic resistance 2002 ASM Press Washington, DC 101 132
2. A. Molander, C. Reit, G. Dahlén, and T. Kvist Microbiological status of root-filled teeth with apical periodontitis Int Endod J 31 1998 1 7
3. G. Sundqvist, D. Figdor, S. Persson, and U. Sjögren Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment Oral Surg Oral Med Oral Pathol 85 1998 86 93
4. E.T. Pinheiro, B.P. Gomes, C.C. Ferraz, E.L. Sousa, F.B. Teixeira, and F.J. Souza-Filho Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 36 2003 1 11
5. J.F. Siqueira Jr., and I.N. Rocas Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97 2004 85 94
6. T.E. Rams, D. Feik, V. Young, B.F. Hammond, and J. Slots Enterococci in human periodontitis Oral Microbiol Immunol 7 1992 249 252
7. C.M. Sedgley, S.L. Lennan, and D.B. Clewell Prevalence, phenotype and genotype of oral enterococci Oral Microbiol Immunol 19 2004 95 101
8. C.A. Heid, J. Stevens, K.J. Livak, and P.M. Williams Real-time quantitative PCR Genome Res 6 1996 986 994
9. R. Higuchi, C. Fockler, G. Dollinger, and R. Watson Kinetic PCR analysis: real-time monitoring of DNA amplification reactions Biotechnology (NY) 11 1993 1026 1030
10. C.E. Shelburne, A. Prabhu, R.M. Gleason, B.H. Mullally, and W.A. Coulter Quantitation of Bacteroides forsythus in subgingival plaque: Comparison of immunoassay and quantitative polymerase chain reaction J Microbiol Methods 39 2000 97 107
11. S.R. Lyons, A.L. Griffin, and E.J. Leys Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria J Clin Microbiol 38 2000 2362 2365
12. H. Maeda, C. Fujimoto, Y. Haruki, T. Maeda, S. Kokeguchi, and M. Petelin Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria FEMS Immunol Med Microbiol 39 2003 81 86
13. S.A. Bustin Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems J Mol Endocrinol 29 2002 23 39
14. R. Patel, K.E. Piper, M.S. Rouse, J.M. Steckelberg, J.R. Uhl, and P. Kohner Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates J Clin Microbiol 36 1998 3399 3407
15. A. Molander, P. Lundquist, P. Papapanou, G. Dahlén, and C. Reit A protocol for polymerase chain reaction detection of Enterococcus faecalis and Enterococcus faecium from the root canal Int Endod J 35 2002 1 6
16. D. Higgins, J. Thompson, T. Gibson, J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res 22 1994 4673 4680
17. M.J. Duncan Genomics of oral bacteria Crit Rev Oral Biol Med 14 2003 175 187
18. S.F. Altschul, W. Gish, W. Miller, E.W. Myers, and D.J. Lipman Basic local alignment search tool J Mol Biol 215 1990 403 410
19. Gish W. (1996-2003) http://blast.wustl.edu.
20. J.A. Klappenbach, P.R. Saxman, J.R. Cole, and T.M. Schmidt rrndb: The Ribosomal RNA Operon Copy Number Database Nucleic Acids Res 29 2001 181 184
21. N. Wellinghausen, B. Wirths, A.R. Franz, L. Karolyi, R. Marre, and U. Reischl Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes Diagn Microbiol Infect Dis 48 2004 229 241
22. F. Ponchel, C. Toomes, K. Bransfield, F.T. Leong, S.H. Douglas, and S.L. Field Real-time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications, and micr gene deletions BMC Biotechnol 3 2003 18
23. E. Frahm, and U. Obst Application of the flourogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples J Microbiol Methods 52 2003 123 131
24. V.H. van der Velden, A. Hochhaus, G. Cazzaniga, T. Szczepanski, J. Gabert, and J.J. van Dongen Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects Leukemia 17 2003 1013 1034
25. J. van Houte, J. Lopman, and R. Kent The predominant cultivable flora of sound and carious human root surfaces J Dent Res 73 1994 1727 1734
26. K. Varadaraj, and D. Skinner Denaturants or cosolvents improve the specificity of PCR amplification of a G+C-rich DNA using genetically engineered DNA polymerases Gene 140 1994 1 5
27. M. Jung, J.M. Muche, A. Lukowsky, K. Jung, and S.A. Loening Dimethyl sulfoxide as additive for real-time polymerase chain reaction analysis with SYBR Green I dye Anal Biochem 289 2001 292 295
28. J. Vandesompele, and A. De Paepe Spelemen F. Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR Green I real-time RT-PCR Anal Biochem 303 2002 95 98
29. S.M. Paule, W.E. Trick, F.C. Tenover, M. Lankford, S. Cunningham, and V. Stosor Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci J Clin Microbiol 41 2003 4805 4807
30. K. Boutaga, A.J. van Winkelhoff, C.M. Vandenbroucke-Grauls, and P.H. Savelkoul Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples J Clin Microbiol 41 2003 4950 4954