Autofluorescence as a viability marker for detection of bacterial spores

Frontiers in Bioscience

Volumn 10, Issue 2, 2005, Pages 1647-1653

  Laflamme, Christian ^a   Verreault, Daniel ^a   Lavigne, Sophie ^a   Trudel, Luc ^c   Ho, Jim ^b   Duchaine, Caroline ^a,c,d  

^a LAVAL UNIVERSITY   (Canada)

^b DEFENCE RESEARCH AND DEVELOPMENT CANADA   (Canada)

^c UNIVERSITÉ LAVAL   (Canada)

^d LAVAL UNIVERSITY   (Canada)

Author keywords

Bacillus anthracis; Biological terrorism; Flow cytometry sorting; Intrinsic fluorescence; Real time detection; Ultraviolet laser

Indexed keywords

DIPICOLINIC ACID;

ARTICLE; AUTOFLUORESCENCE; BACILLUS ANTHRACIS; BACTERIAL CELL WALL; BACTERIAL SPORE; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL MOTILITY; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; LIGHT IRRADIANCE; NONHUMAN; PHOTOSTIMULATION; ULTRAVIOLET SPECTROSCOPY;

BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS);

EID: 17344373550     PISSN: 27686701     EISSN: 27686698     Source Type: Journal    
DOI: 10.2741/1648     Document Type: Article

References (33)

1. Jernigan D.B., P.L. Raghunathan, B.P. Bell, R. Brechner, E.A. Bresnitz, J.C., M. Butler, C. Cetron, M. Cohen, T. Doyle, M. Fischer, C. Greene, K.S. Griffith, J. Guarner, J.L. Hadler, J.A. Hayslett, R. Meyer, L.R. Petersen, M. Phillips, R. Pinner, T. Popovic, C.P. Quinn, J. Reefhuis, D. Reissman, N. Rosenstein, A. Schuchat, W.J. Shieh, L. Siegal, D.L. Swerdlow, F.C. Tenover, M. Traeger, J.W. Ward, I. Weisfuse, S. Wiersma, K. Yeskey, S. Zaki, D.A. Ashford, B.A. Perkins, S. Ostroff, J. Hughes, D. Fleming, J.P. Koplan & J.L. Gerberding: Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings. Emerg Infect Dis 8:1019-1028 (2002)
2. Barer M.R & C.R. Harwood: Bacterial viability and culturability. Adv. Microb. Physiol. 41:94-126 (1999)
3. Amann, R.I., W. Ludwig & K.H. Schleifer: Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59: 143-169 (1995)
4. Davey H.M., A.S. Kaprelyants & D.B. Kell: Flow Cytometric Analysis, using Rhodamine 123, of Micrococcus luteus at Low Growth Rate in Chemostat Culture. In: Flow Cytometry in Microbiology. Eds: Loyd D. London: Springer-Verlag. 83-93 (1993)
5. Edwards C.: Problems posed by natural environments for monitoring microorganisms. Mol. Biotechnol. 15:211-223 (2000)
6. Bunthof C.J., K.B. Bloemen, P. Breewer, F. M Rombouts & T. Abee: Flow cytometry assessment of viability of lactic acid bacteria. Appl. Environ. Microbiol. 67: 2326-2335 (2001)
7. Keer J.T. & L. Birch: Molecular methods for the assessment of bacterial viability. J. Microbiol. Methods 53: 175-183 (2003)
8. Ho J.: Future of biological aerosol detection. Anal. Chim. Acta. 21770: 1-24 (2002)
9. Hairston P.P., J. Ho & F.R. Quant: Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. J. aerosol Sci. 28:471-481 (1997)
10. Thorell B.: Intracellular red-ox steady states as basis for cell characterization by flow cytofluorometry. Blood Cells. 6:745-51 (1980)
11. Chen G., P. Nachman, R. G. Pinnick, S.C. Hill & R.K. Chang: Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation. Optics letters 21: 1307-1309 (1996)
12. Horvath J.J., S.A. Glazier & C.J. Spangler: In situ fluorescence cell mass measurements of Saccharomyces cerevisiae using cellular tryptophan. Biotechnol. Prog. 9: 666-670 (1993)
13. Benson R.C., R.A. Meyer, M.E. Zaruba & G.M. Mckhann: Cellular autofluorescence - Is it due to flavins ? J. Histochem. Cytochem. 27: 44-48 (1979)
14. Ho J. & G. Fisher: Detection of BW agents: flow cytometry measurement of Bacillus subtilis (BG) spore fluorescence. Defence Research Establishment Suffield Memorandum 1421. (1993)
15. Ho J., M. Spence & P. Hairston: Measurement of Biological Aerosol with a Fluorescent Aerodynamic Particle Sizer (FLAPS): Correlation of Optical Data with Biological Data. In: Rapid Methods for Analysis of Biological Materials in the Environment. Eds: Stopa P.J., Bartoszcze M.A., Kluwer Academic Publishers, Netherlands 177-201 (2000)
16. Chaiyanan S., S. Chaiyanan, A. Huq, T. Maugel, & R.R. Colwell Viability of the nonculturable Vibrio cholera O1 & O139. Syst. Appl. Microbiol. 24: 331-341 (2001)
17. Comas-Riu J. & J. Vives-Rego: Cytometric monitoring of growth, sporogenesis and spore cell sorting in Paenibacillus polymyxa (formely Bacillus polymyxa). J. Appl. Microbiol. 92:475-481 (2002)
18. Laflamme C., S. Lavigne, J. Ho & C. Duchaine : Assessment of bacterial endospore viability with fluorescent dyes. J. Appl. Microbiol. 96, 684-692 (2004)
19. Ponce A.: Live/Dead Bacterial Spore Assay Using DPA-Triggered Tb Luminescence. NASA Tech Brief Vol. 27 No. 3 (2003)
20. Laflamme C., J. Ho, M. Veillette, M.C. de Latrémoille, D. Verreault, A. Mériaux, & C. Duchaine : Flow cytometry analysis of germinating Bacillus spores using membrane potential dye. Arch Microbiol. In press. (2004)
21. Aubin J.E.: Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27: 36-43 (1979)
22. th American Society For Microbiology, General Meeting, New Orleans, LA. (2004)
23. Rosen D.L., C. Sharpless & L.B. McGown: Bacterial Spore Detection and Determination by Use of Terbium Dipicolinate Photoluminescence. Anal. Chem. 69, 1082-1085 (1997)
24. Dalterio R.A., W.H. Nelson, D. Britt, J. Sperry, D. Psaras, J.F. Tanguay & S.L Suib: Steady-state and decay characteristics of protein tryptophan fluorescence from bacteria. Appl. Spectro. 40: 86-90 (1986)
25. Leblanc L. & E. Dufour: Monitoring the identity of bacteria using their autofluorescence. FEMS Microbiol. Letters. 211: 147-153 (2002)
26. Caiola M.G., R. Ocampo-Friedmann & E.I. Friedmann: Cytology of long-term desiccation in the desert cyanobacterium Chroococcidiopsis (Chroococcales). Phycologia 32: 315-322 (1993)
27. Iwami Y., S. Takahashi-Abbe, N. Takahashi, T. Yamada, N. Kano & H. Mayanagi: The time-course of acid excretion, levels of fluorescence dependent on cellular nicotinamide adenine nucleotide and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air in the presence of glucose and sorbitol. Oral Microbiol Immunol. 16:34-39 (2001)
28. Shimazaki J., R.A. Laing, K. Tsubota & K.R. Kenyon: Non-invasive assessment of the donor corneal endothelial using ocular redox fluorometry. Br. J. Ophthalmol. 80: 69-73 (1996)
29. Petit P.X., M.C. Gendron, N. Schrantz, N. Metivier, D. Metivier, G. Kroemers, Z. Maciorowska, F. Sureau, & S. Koester: Oxidation of pyridine nucleotides during Fas and ceramide-induced apoptosis in jurkat cells: correlation with changes in mitochondria, glutathione depletion, intracellular acidification and caspase 3 activation. Biochem. J. 353: 357-367 (2001)
30. Eng J., R.M. Lynch & R.S. Balaban: Nicotiamide adenine dinucleotide fluorescence spectroscopy and imaging in isolated cardiac myocytes. Biophy. J. 55: 621-630 (1989)
31. Tokunaga Y., N. Ozaki, S. Wakashiro, I. Ikai, T. Morimoto, Y. Shimahara, Y. Kamiyama, Y. Yamaoka, K. Ozawa & Y. Nakase: Fluorometric study for the noninvasive determination of cellular viability in perfused rat liver. Transplantation. 44:701-706 (1987)
32. Setlow P.: Mechanisms which contribute to the long-term survival of spores of Bacillus species. J. Appl. Bacteriol. Symp. Suppl. 76, 49S-60S (1994)
33. Hobbie J.E., R.J. Daley & S. Jasper: Use of nucleopore filtrers for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1225-1228 (1977)