A novel approach for over-expression, characterization, and isotopic enrichment of a homogeneous species of acyl carrier protein from Plasmodium falciparum

Biochemical and Biophysical Research Communications

Volumn 330, Issue 4, 2005, Pages 1019-1026

  Sharma, Shailendra Kumar ^a   Modak, Rahul ^b   Sharma, Shilpi ^a   Sharma, Alok Kumar ^a   Sarma, Siddhartha P ^a   Surolia, Avadhesha ^a   Surolia, Namita ^b  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Acyl ACPs; Apo ACP; Holo ACP; NMR; Plasmodium falciparum; Protein expression

Indexed keywords

ACYL CARRIER PROTEIN; ISOPROTEIN;

ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTON NUCLEAR MAGNETIC RESONANCE;

EID: 17044372337     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.03.094     Document Type: Article

References (41)

1. S. Smith, A. Witkowski, and A.K. Joshi Structural and functional organization of the animal fatty acid synthase Prog. Lipid Res. 42 2003 289 317
2. C.O. Rock, and J.E. Cronan Escherichia coli as a model for the regulation of dissociable (type II) fatty acid biosynthesis Biochim. Biophys. Acta 1302 1996 1 16
3. N. Surolia, and A. Surolia Triclosan offers protection against blood stages of malaria by inhibiting enoyl-ACP reductase of Plasmodium falciparum Nature Med. 7 2001 167 173
4. A. Surolia, T.N.C. Ramya, V. Ramya, and N. Surolia 'FAS't inhibition of malaria Biochem. J. 383 2004 401 412
5. D.J. Prescott, and P.R. Vegelos Acyl carrier protein Adv. Enzymol. Relat. Areas Mol. Bio. 36 1972 269 311
6. B. Shen, R.G. Summers, H. Gramajo, M.J. Bibb, and C.R. Hutichinson Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polypetide synthase J. Bacteriol. 174 1992 3818 3821
7. C.O. Rock, and S. Jackowski Regulation of phospholipid synthesis in Escherichia coli: composition of the acyl-acyl carrier protein pool in vivo J. Biol. Chem. 257 1982 10759 10765
8. M.S. Anderson, and C.R. Raetz Biosynthesis of lipid A precursors in Escherichia coli: a cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine J. Biol. Chem. 262 1987 5159 5169
9. S.W. Jordan, and J.E. Cronan A new metabolic link: the acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria J. Biol. Chem. 272 1997 17903 17906
10. I. Sanyal, S.L. Lee, and D.H. Flint Biosynthesis of pimeloyl-CoA, a biotin precursor in Escherichia coli, follows a modified fatty acid synthesis pathway: C-labeling studies J. Am. Chem. Soc. 116 1994 2637 2638
11. A.L. Schaefer, D.L. Val, B.L. Hanzelka, J.E. Cronan Jr., and E.P. Greenberg Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein Proc. Natl. Acad. Sci. USA 93 1996 9505 9509
12. J.P. Issartel, V. Koronakis, and C. Huges Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatty acylation Nature 351 1991 759 761
13. O. Geiger, H.P. Spaink, and E.P. Kennedy Isolation of the Rhizobium leguminosarum NodF nodulation protein: NodF carries a 4′- phosphopantetheine prosthetic group J. Bacteriol. 173 1991 2872 2878
14. Y.M. Zhang, H. Marrakchi, S.W. White, and C.O. Rock The application of computational methods to explore the diversity and structure of bacterial fatty acid synthase J. Lipid Res. 44 2003 1 10
15. R.S. Flugel, Y. Hwangbo, R.H. Lambalot, J.E. Cronan Jr., and C.T. Walsh Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli J. Biol. Chem. 275 2000 959 968
16. K.D. Parris, L. Lin, A. Tam, R. Mathew, J. Hixon, M. Stahl, C.C. Fritz, J. Seehra, and W.S. Somers Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites Structure 8 2000 883 895
17. Y.M. Zhang, B. Wu, J. Zheng, and C.O. Rock Key residues responsible for acyl carrier protein and β-ketoacyl-acyl carrier protein reductase (FabG) interaction J. Biol. Chem. 278 2003 52935 52943
18. R.B. Hill, K.R. MacKenzie, J.M. Flanagan, J.E. Cronan Jr., and J.H. Prestegard Overexpression, purification, and characterization of Escherichia coli acyl carrier protein and two mutant proteins Protein Expr. Purif. 6 4 1995 394 400
19. J.A. Broadwater, and B.G. Fox Spinach holo-acyl carrier protein:overproduction and phosphopantetheinylation in Escherichia coli BL21(DE3), in vitro acylation, and enzymatic desaturation of histidine-tagged isoform I Protein Expr. Purif. 15 3 1999 314 326
20. S.A. Morris, W.P. Revill, J. Staunton, and P.F. Leadlay Purification and separation of holo- and apo-forms of Saccharopolyspora erythraea acyl-carrier protein released from recombinant Escherichia coli by freezing and thawing Biochem. J. Pt 2 1993 521 527
21. L. Kremer, K.M. Nampoothiri, S. Lesjean, L.G. Dover, S. Graham, J. Betts, P.J. Brennan, D.E. Minnikin, C. Locht, and G.S. Besra Biochemical characterization of acyl carrier protein (AcpM) and malonyl-CoA: AcpM transacylase (mtFabD), two major components of Mycobacterium tuberculosis fatty acid synthase II J. Biol. Chem. 276 2001 27967 27974
22. S.T. Prigge, X. He, L. Gerena, N.C. Waters, and K.A. Reynolds The initiation steps of type II fatty acid synthase in Plasmodium falciparum are catalyzed by pfACP, pfMAT, and pfKASIII Biochemistry 42 4 2003 1160 1169
23. N.C. Waters, K.M. Kopydlowski, T. Guszczynski, L. Wei, P. Sellers, J.T. Ferlan, P.J. Lee, Z. Li, C.L. Woodard, S. Shallom, M.J. Gardner, and S.T. Prigge Functional characterization of the acyl carrier protein (PfACP) and beta-ketoacyl ACP synthase III (PfKASIII) from Plasmodium falciparum Mol. Biochem. Parasitol. 123 2 2002 85 94
24. W. Trager, and J.B. Jenson Human malaria parasites in continuous culture Science 193 1976 673 675
25. P. Chomczynski, and N. Sacchi Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal. Biochem. 162 1987 156 159
26. D. Post-Beittenmiller, J.G. Jaworski, and J.B. Ohlrogge In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis J. Biol. Chem. 266 1991 1858 1865
27. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
28. C.O. Rock, and J.L. Garwin Preparative enzymatic synthesis and hydrophobic chromatography of acyl-acyl carrier protein J. Biol. Chem. 254 15 1979 7123 7128
29. J.E. Cronan Jr., and L.A. Klages Chemical synthesis of acyl thioesters of acyl carrier protein with native structure Proc. Natl. Acad. Sci. USA 78 9 1981 5440 5444
30. J. Shanklin Over expression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form Protein Expr. Purif. 18 3 2000 355 360
31. T.I. Hwang, and A.J. Shaka Water suppression that works. Excitation sculpting using arbitrary wave-forms and pulsed-field gradients J. Magn. Reson. A 112 1995 275 279
32. M. Wittekind, and L. Muller HNCACB, a high-sensitivity 3D NMR experiment to correlate amide-proton and nitrogen resonances with the alpha- and beta-carbon resonances in proteins J. Magn. Reson. B 101 1994 201 205
33. D.R. Muhandiram, and L.E. Kay Gradient-enhanced triple-resonance three-dimensional NMR experiments with improved sensitivity J. Magn. Reson. B 103 1994 203 216
34. F. Delagilo, S. Grzesiek, G.W. Vuister, G. Zhu, J. Pfeifer, and A. Bax NMRPipe: a multidimensional spectral processing system based on UNIX pipes J. Biomol. NMR 3 1995 277 293
35. P.J. Kraulis, P.J. Domaille, S.L. Campbell-Burk, T. van Aken, and E.D. Laue Solution structure and dynamics of Ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy Biochemistry 33 1994 3515 3531
36. S.A. Ralph, G.G. Van Dooren, R.F. Waller, M.J. Crawford, M.J. Fraunholz, B.J. Foth, C.J. Tonkin, D.S. Roos, and G.I. McFadden Metabolic maps and functions of the Plasmodium falciparum apicoplast Nat. Rev. Microbiol. 2 3 2004 203 216
37. R.F. Waller, M.B. Reed, A.F. Cowman, and G.I. McFadden Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway EMBO J. 19 2000 1794 1802
38. S.K. Sharma, M. Kapoor, T.N.C. Ramya, S. Kumar, G. Kumar, R. Modak, S. Sharma, N. Surolia, and A. Surolia Identification, characterization, and inhibition of Plasmodium falciparum beta-hydroxyacyl-acyl carrier protein dehydratase [FabZ] J. Biol. Chem. 278 46 2003 45661 45671
39. + Biochem. J. 381 Pt 3 2004 725 733
40. M.J. Gardner Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum Science 282 1998 1126 1132
41. H.C. Wong, G. Liu, Y.M. Zhang, C.O. Rock, and J. Zheng The solution structure of acyl carrier protein from Mycobacterium tuberculosis J. Biol. Chem. 277 2002 15874 15880