A three-dimensional organotypic culture of the human uterine exocervix for studying mucosal epithelial differentiation and migrating leukocytes

Differentiation

Volumn 72, Issue 4, 2004, Pages 138-149

  Michelini, Monica ^a,b   Rosellini, Alfredo ^a,b   Simoncini, Tommaso ^c   Papini, Sandra ^a,b   Revoltella, Roberto P ^a  

^a INSTITUTE OF BIOMEDICAL TECHNOLOGIES   (Italy)

^b Farmigea SpA   (Italy)

^c UNIVERSITY OF PISA   (Italy)

Author keywords

Exocervix; Mucosal leukocytes; Squamous epithelium reconstitution; Three dimensional organotypic culture

Indexed keywords

CD31 ANTIGEN; CD45 ANTIGEN; CYTOKERATIN; ESTROGEN RECEPTOR ALPHA; INVOLUCRIN; KI 67 ANTIGEN; PROTEIN P63; VIMENTIN; ANTIPORTER; DNA BINDING PROTEIN; PHOSPHOPROTEIN; PROTEIN PRECURSOR; SLC4A3 PROTEIN, HUMAN; TP73L PROTEIN, HUMAN; TRANSACTIVATOR PROTEIN; TUMOR SUPPRESSOR PROTEIN;

ARTICLE; CELL DIFFERENTIATION; CELL HETEROGENEITY; CELL MIGRATION; EPITHELIUM; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; LEUKOCYTE; MUCOSA CELL; PRIORITY JOURNAL; TISSUE CULTURE; UTERINE CERVIX; CHEMISTRY; CYTOLOGY; FEMALE; IN VITRO STUDY; METHODOLOGY; MUCOSA; NEUTROPHIL CHEMOTAXIS; ORGAN CULTURE TECHNIQUE; TUMOR SUPPRESSOR GENE;

BOVINAE;

ANTIGENS, CD31; ANTIGENS, CD45; ANTIPORTERS; CERVIX UTERI; CHEMOTAXIS, LEUKOCYTE; DNA-BINDING PROTEINS; ESTROGEN RECEPTOR ALPHA; FEMALE; GENES, TUMOR SUPPRESSOR; HUMANS; KI-67 ANTIGEN; MUCOUS MEMBRANE; ORGAN CULTURE TECHNIQUES; PHOSPHOPROTEINS; PROTEIN PRECURSORS; TRANS-ACTIVATORS; TUMOR SUPPRESSOR PROTEINS; VIMENTIN;

EID: 16644377032     PISSN: 03014681     EISSN: 14320436     Source Type: Journal    
DOI: 10.1111/j.1432-0436.2004.07204001.x     Document Type: Article

References (61)

1. Azumi, N. and Battifora, H. (1987) The distribution of vimentin and keratin in epithelial and nonepithelial neoplasms. A comprehensive immunohistochemical study on formalin- and alcohol-fixed tumors. Am J Clin Pathol 88:286-296.
2. Belayet, H.M., Kanayama, N., Khatun, S., Sumimoto, K., Kobayashi, T. and Terao, T. (1999) Binding of interleukin-8 to heparan sulphate enhances cervical maturation in rabbits. Mol Hum Reprod 3:261-269.
3. Brackin, M.N., Cruse, J.M., Lewis, R.E., Hines, R.S., Stopple, J.A. and Cowan, B.D. (2002) Quantitative analysis of adhesion molecules on cellular constituents of the human uterine microenvironment under the influence of estrogen and procesterone. Exp Mol Pathol 72:91-114.
4. Cattoretti, G., Becker, M.H.G., Key, G., Duchrow, M., Shulter, C., Galle, J. and Gerdes, J. (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 168:357-363.
5. Chen, S.S., Revoltella, R.P., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J. and Margolis, L. (2003) Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. Stem Cells 21:281-295.
6. Chilton, B.S., Deeb, E.A. and Dalley, B.K. (1991) Progesterone regulates epidermal growth factor receptor on mucous secreting cells in the rabbit endocervix. Endocrinology 4:2094-2100.
7. Chishima, T., Yang, M., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Shimada, H., Moossa, A.R., Penman, S. and Hoffman, R.M. (1997) Governing step of metastasis visualized IH vitro. Proc Natl Acad Sci USA 94:11573-11576.
8. Cleaver, O. and Melton, D.A. (2003) Endothelial signaling during development. Nat Med 9:661-668.
9. Collins, K.B., Patterson, B.K., Naus, G.J., Landers, D.V. and Gupta, P. (2000) Development of an in vitro organ culture model to study transmission of HIV-1 in the female genital tract. Nat Med 6:475-479.
10. Darwiche, N., Celli, G., Sly, L., Lancillotti, F. and De Luca, L.M. (1993) Retinoid status controls the appearance of reserve cells and keratin expression in mouse cervical epithelium. Cancer Res 53:2287-2299.
11. Dellas, A., Moch, H., Schultheiss, E., Feichter, G., Almendral, A.C., Gudat, F. and Torhorst, J. (1997) Angiogenesis in cervical neoplasia: microvessel quantitation in precancerous lesions and invasive carcinomas with clinicopathological correlations. Gynecol Oncol 67:27-33.
12. DeYoung, B.R., Swanson, P.E., Argenyi, Z.B., Ritter, J.H., Fitzgibbon, J.F., Stahl, D.J., Hoover, W. and Wick, M.R. (1995) CD31 immunoreactivity in mesenchymal neoplasms of the skin and subcutis: report of 145 cases and review of putative immunohistologic markers of endothelial differentiation. J Cutan Pathol 22:215-222.
13. Di Como, C.J., Urist, M.J., Babayan, I., Drobnjak, M., Hedvat, C.V., Teruya-Feldstein, J., Pohar, K., Hoos, A. and Cordon-Cardo, C. (2002) p63 expression profiles in human normal and tumor tissues. Clin Cancer Res 8:494-501.
14. Ferenczy, A. and Wright, C. (1994) Anatomy and histology of the cervix. In: Kurman, J. and Blaustein, A. (eds.) Blaustein's pathology of the female genital tract. Springer, New York, pp. 185-201.
15. Fesus, L. (1993) Biochemical events in naturally occurring forms of cell death. FEBS Lett 328:1-5.
16. Fesus, L., Davies, P.J. and Piacentini, M. (1991) Apoptosis: molecular mechanisms in programmed cell death. Eur J Cell Biol 56:170-177.
17. Freshney, R.I. (1994) Culture of animal cells. 3rd ed. Wiley-Liss, New York.
18. Glushakova, S., Baibakov, B., Margolis, L.B. and Zimmerberg, J. (1995) Infection of human tonsil histocultures: a model for HIV pathogenesis. Nat Med 12:1320-1322.
19. Gorodeski, G.I., Eckert, R.L., Utian, W.H., Sheean, L. and Rorke, E.A. (1989) Retinoids, sex steroids and glucocorticoids regulate ectocervical cell envelope formation but not the level of the envelope precursor, involucrin. Differentiation 42:75-80.
20. Gorodeski, G.I., Eckert, R.L., Utian, W.H. and Rorke, E.A. (1990a) Maintenance of in vivo-like keratin expression, sex steroid responsiveness, and estrogen receptor expression in cultured human ectocervical epithelial cells. Endocrinology 126:399-406.
21. Gorodeski, G.I., Eckert, R.L., Utian, W.H., Sheean, L. and Rorke, E.A. (1990b) Cultured human ectocervical epithelial cell differentiation is regulated by the combined direct actions of sex steroids, glucocorticoids, and retinoids. J Clin Endocrinol Metab 70:1624-1630.
22. Gorodeski, G.I., Romero, M.F., Hopfer, U., Rorke, E., Utian, W.H. and Eckert, R.L. (1994) Human uterine cervical epithelial cells grown on permeable support-a new model for the study of differentiation. Differentiation 52:107-118.
23. Hall, P.A., d'Ardenne, A.J. and Stansfeld, A.G. (1998) Paraffin section immunohistochemistry. I. Non-Hodgkin's lymphoma. Histopathology 13:149-160.
24. Hoffman, R.M. (1991) Three-dimensional gel-supported native-state histoculture for evaluation of tumor-specific pharmacological activity: principles, practice and possibilities. J Cell Pharmacol 2:189-202.
25. Hudson, D.L., Weiland, K.L., Dooley, T.P., Simon, M. and Watt, F.M. (1992) Characterization of eight monoclonal antibodies to involucrin. Hybridoma 11:367-379.
26. Ince, T.A., Cviko, A.P., Quade, B.J., Yang, A., McKeon, F.D., Mutter, G.L. and Crum, C.P. (2002) p63 coordinates anogenital modeling and epithelial cell differentiation in the developing female urogenital tract. Am J Pathol 161:1111-1117.
27. Jacinto, A., Martinez-Arias, A. and Martin, P. (2001) Mechanisms of epithelial fusion and repair. Nat Cell Biol 3:117-123.
28. Kalinin, A.E., Kajava, A.V. and Steinert, P.M. (2002) Epithelial barrier function: assembly and structural features of the cornified cell envelope. Bioessays 24:789-800.
29. Kanai, M., Shiozawa, T., Xin, L., Nikaido, T. and Fujii, S. (1998) Immunohistochemical detection of sex steroid receptors, cyclins, and cyclin-dependent kinases in the normal and neoplastic squamous epithelia of the uterine cervix. Cancer 82:1709-1719.
30. Kino-Oka, M. and Prenosil, J.E. (2000) Development of an on-line monitoring system of human keratinocyte growth by image analysis and its application to bioreactor culture. Biotechnol Bioeng 67:234-239.
31. Leighton, J. (1957) Contributions of tissue culture studies to an understanding of the biology of cancer: a review. Cancer Res 17:929-941.
32. Lillie, J.H., MacCallum, D.K. and Jepsen, A. (1988) Growth of stratified squamous epithelium on reconstituted extracellular matrices: long-term culture. J Invest Dermatol 90:100-109.
33. Lin, C.Q. and Bissell, M.J. (1993) Multi-faceted regulation of cell differentiation by extracellular matrix. FASEB J 7:737-743.
34. Little, N.A. and Jochemsen, A.G. (2002) p63. Int J Biochem Cell Biol 34:6-9.
35. Ma, Z., Lu, F.X., Torten, M. and Miller, C.J. (2001) The number and distribution of immune cells in the cervicovaginal mucosa remain constant throughout the menstrual cycle of rhesus macaques. Clin Immunol 100:240-249.
36. Malkevitch, N., McDermott, D.H., Yi, Y., Grivel, J.C., Schols, D., De Clercq, E., Murphy, P.M., Glushakova, S., Collman, R.G. and Margolis, L. (2001) Coreceptor choice and T cell depletion by R5, X4, and R5X4 HIV-1 variants in CCR5-deficient (CCR5532) and normal human lymphoid tissue. Virology 2:239-247.
37. Margolis, L., Hatfill, S., Chuaqui, R., Vocke, C., Emmert-Buck, M., Linehan, W.M. and Duray, P.H. (1999) Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactor. J Urol 161:290-297.
38. Martin, P. (1997) Wound healing-aiming for perfect skin regen-eration. Science 276:75-81.
39. Meyers, C., Frattini, M.G., Hudson, J.B. and Laimins, L.A. (1992) Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation. Science 257:971-973.
40. Miller, C.J., McChesney, M. and Moore, P.F. (1992) Langerhans cells, macrophages and lymphocytes subsets in the cervix and vagina of rhesus macaques. Lab Invest 67:628-634.
41. Moll, R., Franke, W.W. and Shiller, D.I. (1982) The catalogue of human cytokeratins: pattern of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24.
42. Nielsen, L.N., Hording, U., Daugaard, S., Rasmussen, L.P. and Norrild, B. (1991) Cytokeratin intermediate filament pattern and human papillomavirus type in uterine cervical biopsies with different histological diagnosis. Gynecol Obstet Invest 32:232-238.
43. Odor, D.L. and Blandau, R.J. (1988) Light and electron microscopic observation on the cervical epithelium of the rabbit. I. Am J Anat 181:289-319.
44. Odor, D.L., Horacek, M.J. and Blandau, R.J. (1989) Light and electron microscopic observations on the cervical epithelium of the rabbit: II. Am J Anat 185:343-366.
45. Papini, S., Cecchetti, D., Campani, D., Fitzgerald, W., Grivel, J.C., Chen, S., Margolis, L. and Revoltella, R.P. (2003) Isolation and clonal analysis of human epidermal keratinocyte stem cells in long-term culture. Stem Cells 21:481-494.
46. Papini, S., Rosellini, A., Campani, A., Dematteis, A., Selli, C. and Revoltella, R.P. (2004) Selective growth of epithelial basal cells from human prostate in a three-dimensional organ culture. Prostate 59:383-392.
47. Parenteau, N.L., Bilbo, P., Nolte, C.J., Mason, V.S. and Rosenberg, M. (1992) The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function. Cytotechnology 9:163-171.
48. Payne, S., Kernohan, N.M. and Walker, F. (1996) Proliferation in the normal cervix and in preinvasive cervical lesions. J Clin Pathol 49:667-671.
49. Pellegrini, G., Dellambra, E., Golisano, O., Martinelli, E., Fantozzi, I., Bondanza, S., Ponzin, D., McKeon, F. and De Luca, M. (2001) p63 identifies keratinocyte stem cells. Proc Natl Acad Sci USA 98:3156-3161.
50. Picker, L.J. and Siegelman, M.H. (1993) Lymphoid tissues and organs. In: Paul, W.E. (ed.) Fundamental immunology. 3rd ed. Raven Press, New York, pp. 145-197.
51. Puts, J.J., Moesker, O., de Waal, R.M., Kenemans, P., Vooijs, G.P. and Ramaekers, F.C. (1986) Immunohistochemical identification of Langerhans cells in normal epithelium and in epithelial lesions of the uterine cervix. Int J Gynecol Pathol 5:151-162.
52. Quade, B.J., Yang, A., Wang, Y., Sun, D., Park, J., Sheets, E.E., Cviko, A., Federschneider, J.M., Peters, R., McKeon, F.D. and Crum, C.P. (2001) Expression of the p53 homologue p63 in early cervical neoplasia. Gynecol Oncol 80:24-29.
53. Ryan, P.L., Baum, D.L., Lenhart, J.A., Ohleth, K.M. and Bagnell, C.A. (2001) Expression of uterine and cervical epithelial cadherin during relaxin-induced growth in pigs. Reproduction 122:929-937.
54. Sannino, P. and Shousha, S. (1994) Demonstration of oestrogen receptors in paraffin wax sections of breast carcinoma using the monoclonal antibody 1D5 and microwave oven processing. J Clin Pathol 47:90-92.
55. Schock, F. and Perrimon, N. (2002) Molecular mechanisms of epithelial morphogenesis. Annu Rev Cell Dev Biol 18:463-493.
56. Serra, V., Ramirez, A.A., Lara, C., Marzo, C., Castells, A. and Bonilla-Musoles, F. (1990) Distribution of involucrin in normal and pathological human uterine cervix. Gynecol Oncol 36:34-42.
57. Shen, H., Xu, P., Yu, W., Liu, Y. and Wu, D. (2003) Effects of p-nonylphenol on expression of ER protein and ER α mRNA of MCF-7 cells (article in Chinese). Sichuan Da Xue Xue Bao Yi Xue Ban 4:641-644.
58. Sivard, P., Dezutter-Dambuyant, C., Kanitakis, J., Mosnier, J.F., Hamezeh, H., Bechetoille, N., Berthier, O., Sabido, O., Schmitt, D., Genin, C. and Misery, L. (2003) In vitro reconstructed mucosa-integrating Langerhans' cells. Exp Dermatol 12:346-355.
59. Stern, J.E., Givan, A.L., Gonzalez, J.L., Harper, D.M., White, H.D. and Wira, C.R. (1998) Leukocytes in the cervix: a quantitative evaluation of cervicitis. Obstet Gynecol 91:987-992.
60. Waggoner, S.E. (2003) Cervical cancer. Lancet 361:2217-2225.
61. Yang, A., Schweitzer, R., Sun, D., Kaghad, M., Walker, N., Bronson, R.T., Tabin, C., Sharpe, A., Caput, D., Crum, C. and McKeon, F. (1999) p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398:714-718.