Increased rate of chondrocyte aggregation in a wavy-walled bioreactor

Biotechnology and Bioengineering

Volumn 88, Issue 6, 2004, Pages 767-777

  Bueno, Ericka M ^a   Bilgen, Bahar ^a   Carrier, Rebecca L ^a   Barabino, Gilda A ^a  

^a Northeastern University   (United States)

Author keywords

Aggregation kinetics; Bioreactor; Cartilage tissue engineering; Chondrocyte aggregation; Residence time distribution

Indexed keywords

AGGLOMERATION; CELL CULTURE; CELLS; GROWTH KINETICS; HYDRODYNAMICS; SHEAR STRESS; SUSPENSIONS (FLUIDS); TISSUE;

CELL CLUMPS; CHONDROCYTE AGGREGATION; SINNER FLASK BIOREACTORS; TISSUE PROPERTIES;

BIOREACTORS;

ANIMAL CELL; ARTICLE; BIOREACTOR; CARTILAGE CELL; CELL AGGREGATION; CELL INTERACTION; CELL KINETICS; CELL SURVIVAL; COMPUTER ANALYSIS; CONTROLLED STUDY; HYDRODYNAMICS; IMAGE ANALYSIS; NONHUMAN; SHEAR STRESS; SURVIVAL RATE; SUSPENSION; TISSUE CULTURE; TISSUE ENGINEERING; WAVY WALLED BIOREACTOR; ANIMAL; CATTLE; CELL CULTURE; CELL MOTION; CELL SIZE; COMPARATIVE STUDY; CULTURE TECHNIQUE; CYTOLOGY; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; FLOW KINETICS; INSTRUMENTATION; KINETICS; MECHANOTRANSDUCTION; METHODOLOGY; PHYSIOLOGY;

ANIMALS; BIOREACTORS; CATTLE; CELL AGGREGATION; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELL SIZE; CELL SURVIVAL; CELLS, CULTURED; CHONDROCYTES; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; KINETICS; MECHANOTRANSDUCTION, CELLULAR; RHEOLOGY; TISSUE ENGINEERING;

EID: 16644365884     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20261     Document Type: Article

References (31)

1. Abbott J, Holtzer H. 1966. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures. J Cell Biol US 28:473-487.
2. Aigner J, Hutzler P, Campoccia D, Pavesio A, Hammer C, Kastenbauer E, Naumann A. 1998. Cartilage tissue engineering with novel nonwoven structured biomaterial based on hyaluronic acid benzyl ester. J Biomed Mater Res 42:172-181.
3. Barabino GA, Metghalchi M, Zhang B. 1993. Mammalian cell culture in a novel bioreactor. Anaheim, CA: ASME-BED, Bioengineering Division, p 1-4.
4. Carver SE, Heath CA. 1999. Influence of intermittent pressure, fluid flow, and mixing on the regenerative properties of articular chondrocytes. Biotechnol Bioeng 65:274-281.
5. Chattopadhyay D, Garcia-Briones M, Venkat RV, Chalmers JJ. 1997. Hydrodynamic properties in bioreactors. In: Hauser H, Wagner R, editors. Mammalian cell biotechnology in protein production. Berlin: Walter de Gruyter. p 319-343.
6. Croughan MS, Wang DIC. 1989. Growth and death in overagitated microcarrier cell cultures. Biotechnol Bioeng 33:731-744.
7. Davisson T, Sah RLY, Ratcliffe A. 2002. Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures. Tissue Eng 8:807-816.
8. Freed LE, Hollander AP, Martin I, Barry JR, Langer R, Vunjak-Novakovic G. 1998. Chondrogenesis in a cell-polymer bioreactor system. Exp Cell Res 240:58-65.
9. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R. 1993a. Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 27:11-23.
10. Freed LE, Marquis JC, Vunjak-Novakovic G, Emmanual J, Langer R. 1994. Composition of cell-polymer cartilage implants. Biotechnol Bioeng 43:605-614.
11. Freed LE, Vunjak-Novakovic G. 1995. Cultivation of cell-polymer tissue constructs in simulated microgravity. Biotechnol Bioeng 46:306-313.
12. Freed LE, Vunjak-Novakovic G. 1997. Tissue culture bioreactors: chondrogenesis as a model system. In: Lanza R, Langer R, Chick W, editors. Principles of tissue engineering: New York: Academic Press, p 151-165.
13. Freed LE, Vunjak-Novakovic G. 2000. Tissue engineering bioreactors. In: Lanza RP, Langer R, Vacanti J, editors. Principles of tissue engineering. New York: Academic Press, p 143-156.
14. Freed LE, Vunjak-Novakovic G, Marquis JC, Langer R. 1993b. Kinetics of chondrocyte growth in cell-polymer implants. Biotechnol Bioeng 43:597-604.
15. Gooch KJ, Kwon JH, Blunk T, Langer R, Freed LE, Vunjak-Novakovic G. 2001. Effects of mixing intensity on tissue-engineered cartilage. Biotechnol Bioeng 72:402-407.
16. Kunas KT, Papoutsakis ET. 1990. Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment. Biotechnol Bioeng 36:476-483.
17. Lavietes BB. 1970. Cellular interaction and chondrogenesis in vitro. Devel Biol US 21:584-610.
18. LeBaron RG, Athanasiou KA. 2000. Ex vivo synthesis of articular cartilage. Biomaterials 21:2575-2587.
19. Levenspiel O. 1972. Chemical reaction engineering. New York: John Wiley & Sons. 578 p.
20. Martin I, Demarteau O, Braccini A. 2002. Recent advances in cartilage tissue engineering: from the choice of cell sources to the use of bioreactors. JSME Int J 45:851-861.
21. Martin I, Dozin B, Quarto R, Cancedda R, Beltrame F. 1997. Computer based technique for cell aggregation analysis and cell aggregation in vitro chondrogenesis. Cytometry 28:141-146.
22. Morris JE. 1976. Cell aggregation rate vs aggregate size. Devel Biol 54:288-296.
23. Moscona A. 1961. Rotation-mediated histogenetic aggregation of dissociated cells. Exp Cell Res 22:455-475.
24. Natarajan B, Metghalchi M, Tangborn A, Barabino GA. 1992. Hydrodynamic evaluation of a novel bioreactor design. Anaheim, CA: ASME-BED, Bioengineering Division, p 1-6.
25. Peel SAF, Chen H, Renlund R, Badylak SF, Kandel RA. 1998. Formation of a SIS-cartilage composite graft in vitro and its use in the repair of articular cartilage defects. Tissue Eng 4:143-155.
26. Saini S, Wick TM. 2003. Concentric cylinder bioreactor for production of tissue engineered cartilage: effect of seeding density and hydrodynamic loading on construct development. Biotechnol Progr 19:510-521.
27. van Kampen GPJ, Veldhuijzen JP. 1982. Aggregated chondrocytes as a model system to study cartilage metabolism. Exp Cell Res 140:441-443.
28. Vunjak-Novakovic G, Freed LE, Biron RJ, Langer R. 1996. Effects of mixing on the composition and morphology of tissue-engineered cartilage. AIChE J 42:850-860.
29. Vunjak-Novakovic G, Martin I, Obradovic B, Treppo S, Grodzinsky AJ, Langer R, Freed LE. 1999. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J Orthop Res 17:130-138.
30. Vunjak-Novakovic G, Obradovic B, Martin I, Bursac PM, Langer R, Freed LE. 1998. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol Progr 14:193-202.
31. Wu S-C. 1999. Influence of hydrodynamic shear stress on microcarrier-attached cell growth: cell line dependency and surfactant protection. Bioproc Eng 21:201-206.