Development of a genetic system in chitinase-producing Streptomyces and the application of an allosamidin-insensitive chitinase gene to homologous overexpression

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 26, Issue 3, 2004, Pages 179-185

  Kawachi, Ryu ^a   Koike, Yuko ^a   Watanabe, Yumi ^a   Nishio, Toshiyuki ^a   Sakuda, Shohei ^b   Nagasawa, Hiromichi ^b   Oku, Tadatake ^a  

^a NIHON UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Allosamidin; Chitin; Chitinase; Homologous overexpression; Streptomyces; Transformation

Indexed keywords

ENZYME KINETICS; ESCHERICHIA COLI; GENES; GLYCEROL; OPTIMIZATION; SUGAR (SUCROSE);

GENETIC SYSTEM; GLYCINE; PROTEIN FOLDING; PROTOPLASTS; TRANSFORMATION SYSTEM;

MOLECULAR BIOLOGY;

ALLOSAMIDIN; CHITINASE; DNA; ENDONUCLEASE; FOCAL ADHESION KINASE; FOCAL ADHESION KINASE 1; GLYCINE; MACROGOL; METHYLTRANSFERASE; SUCROSE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; DRUG DERIVATIVE; ENZYME INHIBITOR; N ACETYLGLUCOSAMINE; RECOMBINANT PROTEIN; RRNA (ADENOSINE O 2' )METHYLTRANSFERASE; RRNA (ADENOSINE-O-2'-)METHYLTRANSFERASE; TRISACCHARIDE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ISOLATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE FUNCTION; GENE OVEREXPRESSION; GENETIC ANALYSIS; GENETIC TRANSFORMATION; METHYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTOPLAST; SEQUENCE HOMOLOGY; STREPTOMYCES; WILD TYPE; ANTIBIOTIC RESISTANCE; BIOSYNTHESIS; CHEMISTRY; DNA METHYLATION; DRUG ANTAGONISM; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES SP.;

ACETYLGLUCOSAMINE; BACTERIAL PROTEINS; CHITINASE; DNA METHYLATION; DRUG RESISTANCE, BACTERIAL; ENZYME INHIBITORS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; METHYLTRANSFERASES; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY; STREPTOMYCES; TRANSFECTION; TRISACCHARIDES;

EID: 16544388633     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:26:3:179     Document Type: Article

References (21)

1. Muzzarelli, R. A. A., Mattioli-Belmonte, M., Pugnaloni, A., and Biagini, G. (1999) Biochemistry, histology and clinical uses of chitins and chitosans in wound healing,in: Chitin and Chitinases (Jollès, P. and Muzzarelli, R. A. A., eds.), Birkhäuser, Basel, Switzerland, pp. 251-264.
2. Felse, P. A. and Panda, T. (1999) Regulation and cloning of microbial chitinase genes. Appl. Microbiol. Biotechnol. 51, 141-151.
3. Sakuda, S., Isogai, A., Matsumoto, S., Suzuki, A., and Koseki, K. (1986) The structure of allosamidin, a novel insect chitinase inhibitor, produced by Streptomyces sp. Tetrahedoron Lett. 27, 2475-2478.
4. Sakuda, S., Isogai, A., Matsumoto, S., and Suzuki, A. (1987) Search for microbial insect growth-regulators. 2. allosamidin, a novel insect chitinase inhibitor. J. Antibiot. 40, 296-300.
5. Henrissat, B. (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280, 309-316.
6. Henrissat, B. and Bairoch, A. (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293, 781-788.
7. Zhou, Z. Y., Sakuda, S., and Yamada, Y. (1992) Biosynthetic-studies on the chitinase inhibitor, allosamidin-origin of the carbon and nitrogen-atoms. J. Chem. Soc. Perk. T. 1, 1649-1652.
8. Ohno, T., Armand, S., Hata, T., et al. (1996) A modular family 19 chitinase found in the prokaryotic organisms Streptomyces griseus HUT 6037. J. Bacteriol. 178, 5065-5070.
9. Saito, A., Fujii, T., Yoneyama, T., et al. (1999) High-multiplicity of chitinase genes in Streptomyces coelicolor A3(2). Biosci. Biotechnol. Biochem. 63, 710-718.
10. Nakanishi, E., Susumu, O., Matsuura, H., Nagasawa, H., and Sakuda, S. (2001) Allosamidin, a chitinase inhibitor produced by Streptomyces, acts as an inducer of chitinase production in its producing strain. Proc. Japan Acad. 77B, 79-82.
11. Kieser, T., Bibb M. J., Buttner, M. J., Chater, K. F., and Hopwood, D. A. (2000) Practical Streptomyces Genetics, The John Innes Foundation, Norwich, UK.
12. Kawachi, R., Nihira, T. and Yamada, Y. (1997) Development of a transformation system in Streptomyces virginiae. Actinomycetol. 11, 46-53.
13. MacNeil, D. J. (1988) Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170, 5607-5612.
14. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
15. Sambrook, J., Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
16. Matsushima, P. and Baltz, R. H. (1989) Streptomyces lipmanii expresses two restriction systems that inhibit plasmid transformation and bacteriophage plaque formation. J. Bacteriol. 171, 3128-3132.
17. Baltz, R. H. (1978) Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J. Gen. Microbiol. 107, 93-102.
18. Matsushima, P. and Baltz, R. H. (1988) Genetic transformation of Micromonospora rosaria by the Streptomyces plasmid pIJ702. J. Antibiot. 41, 583-585.
19. Wehmeier, U. F. (1995) New multifunctional Escherichia coli-Streptomyces shuttle vectors allowing bluewhite screening on XGal plates. Gene 165, 149-150
20. Kawachi, R., Akashi, T., Kamitani, Y., Nihira, T., and Yamada, Y. (2000) Identification of an AfsA homologue (BarX) from Streptomyces virginiae as a pleiotropic regulator controlling autoregulator biosynthesis, virginiamycin biosynthesis and virgini-amycin M1 resistance. Mol. Microbiol. 36, 302-313.
21. Kawachi, R., Wangchaisoonthorn, U., Nihira, T., and Yamada, Y. (2000) Identification by gene disruption analysis of a regulator, VmsR, that controls virginia-mycin biosynthesis in Streptomyces virginiae. J. Bacteriol. 182, 6259-6263.