Assessment of bovine X- and Y-bearing spermatozoa in fractions by discontinuous percoll gradients with rapid fluorescence in situ hybridization

Journal of Reproduction and Development

Volumn 50, Issue 4, 2004, Pages 463-469

  Kobayashi, Jin ^a   Oguro, Hitoshi ^a   Uchida, Hiroshi ^a   Kohsaka, Tetsuya ^b   Sasada, Hiroshi ^c   Sato, Eimei ^c  

^a MIYAGI UNIVERSITY   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

Author keywords

Bovine; FISH; Percoll separation; Semen sexing; Spermatozoa

Indexed keywords

CAFFEINE; PERCOLL; POVIDONE; SILICON DIOXIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL FRACTIONATION; CELL SUBPOPULATION; COW; FERTILIZATION IN VITRO; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENETIC ENGINEERING; NONHUMAN; SEMEN ANALYSIS; SEX DETERMINATION; SEX RATIO; SPERM; SPERMATOZOON; SPERMATOZOON MOTILITY; X CHROMOSOME; Y CHROMOSOME; ANIMAL; ANIMAL DISEASE; CATTLE; CELL SEPARATION; CYTOLOGY; MALE; METHODOLOGY;

ANIMALIA; BOVINAE;

ANIMALS; CATTLE; CELL SEPARATION; FERTILIZATION IN VITRO; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; POVIDONE; SEX PRESELECTION; SILICON DIOXIDE; SPERMATOZOA; X CHROMOSOME; Y CHROMOSOME;

EID: 16444379009     PISSN: 09168818     EISSN: None     Source Type: Journal    
DOI: 10.1262/jrd.50.463     Document Type: Article

References (27)

1. Ericsson RJ, Langevin CN, Nishino M. Isolation of fractions rich in human Y sperm. Nature 1973; 246: 421-424.
2. Quinlivan WLG, Preciado K, Long TL, Sullivan H. Separation of human X and Y spermatozoa by albumin gradients and Sephadex chromatography. Fertil Steril 1982; 37: 104-107.
3. Steeno O, Adimoelja A, Steeno J. Separation of X- and Y-bearing human spermatozoa with the Sephadex gel-filtration method. Andrologia 1975; 7: 95-97.
4. Kaneko S, Yamaguchi J, Kobayashi T, Iizuka R. Separation of human X- and Y-bearing sperm using Percoll density gradient centrifugation. Fertil Steril 1983; 40: 661-665.
5. Iizuka R, Kaneko S, Aoki R, Kobayashi T. Sexing of human sperm by discontinuous Percoll density gradient and its clinical application. Hum Reprod 1987; 2: 573-575.
6. Check JH, Katsoff D. A prospective study to evaluate the efficacy of modified swim-up preparation for male sex selection. Hum Reprod 1993; 8: 211-214.
7. Cran DG, Johnson LA. The predetermination of embryonic sex using flow cytometrically separated X and Y spermatozoa. Hum Reprod Update 1996; 2: 355-363.
8. Windsor DP, Evans G, White IG. Sex predetermination by separation of X and Y chromosome-bearing sperm: a review. Reprod Fertil Dev 1993; 5: 155-171.
9. van Kooij RJ, van Oost BA. Determination of sex ratio of spermatozoa with a deoxyribonucleic acid-probe and quinacrine staining: a comparison. Fertil Steril 1992; 58: 384-386.
10. Vidal F, Moragas M, Catala V, Torello MJ, Santalo J, Calderon G, Gimenez C, Barri PN, Egozcue J, Veiga A. Sephadex filtration and human serum albumin gradients do not select spermatozoa by sex chromosome: a fluorescent in-situ hybridization study. Hum Reprod 1993; 8: 1740-1743.
11. Wang HX, Flaherty SP, Swann NJ, Matthews CD. Assessment of the separation of X- and Y-bearing sperm on albumin gradients using double label fluorescence in-situ hybridization. Fertil Steril 1994; 61: 720-726.
12. Beckett TA, Martin RH, Hoar DI. Assessment of the Sephadex technique for selection of X-bearing human sperm by analysis of sperm chromosomes, deoxyribonucleic acid and Y-bodies. Fertil Steril 1989; 52: 829-835.
13. Lobel SM, Pomponio RJ, Mutter GL. The sex ratio of nomal and manipulated human sperm quantitated by the polmearase chain reaction. Fertil Steril 1993; 59: 387-392.
14. Han TL, Flaherty SP, Ford JH, Matthews CD. Detection of X- and Y-bearing human spermatozoa after motile sperm isolation by swim-up. Fertil Steril 1994; 61: 1181-1182.
15. Wang H, Flaherty SP, Swann NJ, Matthews CD. Discontinuous Percoll gradients enrich X-bearing human spermatozoa: a study using double-label fluorescence in-situ hybridization. Hum Reprod 1994; 9: 1265-1270.
16. Lin SP, Lee RK, Tsai YJ, Hwu YM, Lin MH. Separating X-bearing human spermatozoa through a discontinuous Percoll density gradient proved to be inefficient by double-label fluorescent in situ hybridization. J Assist Reprod 1998; 15: 565-569.
17. Kobayashi J, Kohsaka T, Sasada H, Umezu M, Sato E. Fluorescence in situ hybridization with Y chromosome-specific probe in decondensed bovine spermatozoa. Theriogenology 1999; 52: 1043-1054.
18. Brackett BG, Oliphant G. Capacitation of rabbit spermatozoa in vitro. Biol Reprod 1975; 12: 260-274.
19. Cotinot C, Kirszenbaum M, Leonard M, Gianquinto L, Vaiman M. Isolation of bovine Y-derived sequence: potential use in embryo sexing. Genomics 1991; 10: 646-653.
20. Kobayashi J, Sekimoto A, Uchida H, Wada T, Sasaki K, Sasada H, Umezu M, Sato E. Rapid detection of male-specific DNA sequence in bovine embryos using fluorescence in situ hybridization. Mol Reprod Dev 1998; 51: 390-394.
21. Kaneko S, Oshio S, Kobayashi T, Mohri H, Iizuka R. Buoyancy and sedimentation of human X- and Y-bearing sperm. Arch Androl 1987; 19: 211-215.
22. Watkins AM, Chan PJ, Patton WC, Jacobson JD, King A. Sperm kinetics and morphology before and after fractionation on discontinuous Percoll gradient for sex preselection: computerized analyses. Arch Androl 1996; 37: 1-5.
23. Cui KH, Matthews CD. X larger than Y. Nature 1993; 366: 117-118.
24. Upreti GC, Riches PC, Johnson LA. Attempted sexing of bovine spermatozoa by fractionation on a Percoll density gradient. Gamete Res 1988; 20: 83-92.
25. Aitken RJ, West KM. Analysis of the relationship between reactive oxygen species production and leucocyte infiltration in fractions of human semen separated on Percoll gradients. Int J Androl 1990; 13: 433-451.
26. Penfold LM, Holt C, Holt WV, Welch GR, Cran DG, Johnson LA. Comparative motility of X and Y chromosome-bearing bovine sperm separated on the basis of DNA content by flow sorting. Mol Reprod Dev 1998; 50: 323-327.
27. Hamano K, Uchiyama K. Identification of X and Y bearing bull spermatozoa using chromosomal analysis. Jpn J Anim Reprod 1989; 35: 46-52 (in Japanese).