Separating X-bearing human spermatozoa through a discontinuous Percoll density gradient proved to be inefficient by double-label fluorescent in situ hybridization

Journal of Assisted Reproduction and Genetics

Volumn 15, Issue 9, 1998, Pages 565-569

  Lin, Shau Ping ^a   Lee, Robert Kuo Kuang ^a,b   Tsai, Yuan Jang ^a   Hwu, Yuh Ming ^a   Lin, Ming Huei ^a  

^a MACKAY MEMORIAL HOSPITAL   (Taiwan)

^b CHINA MEDICAL UNIVERSITY   (Taiwan)

Author keywords

Fluorescence in situ hybridization; Percoll; X sperm preselection

Indexed keywords

ADULT; ARTICLE; FLUORESCENCE IN SITU HYBRIDIZATION; GENETIC ENGINEERING; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MALE; PRIORITY JOURNAL; SCORING SYSTEM; SEMEN ANALYSIS; SPERMATOZOON; X CHROMOSOME; Y CHROMOSOME;

EID: 0031766686     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022590321986     Document Type: Article

References (17)

1. McKusick VA: Mendelian Inheritance in Man, 9th ed. Baltimore, Johns Hopkins University Press, 1990
2. Reubinoff BE, Schenker JG: New advances in sex preselection. Fertil Steril 1996;66:343-350
3. Steeno O, Adimoelja A, Steeno J: Separation of X- and Y-bearing human spermatozoa with the Sephadex gel-filtration method. Andrologia 1975;7:95-97
4. Quinlivan WLG, Preciado K, Long TL, Sullivan H: Separation of human X and Y spermatozoa by albumin gradients and Sephadex chromatography. Fertil Steril 1982;37:104-107
5. Kaneko S, Yamaguchi J, Kobayashi T, Iizuka R: Separation of human X- and Y-bearing sperm using Percoll density gradient centrifugation. Fertil Steril 1983;40:661-665
6. Iizuka R, Kaneko S, Aoki R, Kobayashi T: Sexing of human sperm by discontinuous Percoll density gradient and its clinical application. Hum Reprod 1987;2:573-575
7. Johnson, LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD: Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 1993;8:1733-1739
8. Levinson G, Keyvanfar K, Wu JC, Fugger EF, Fields RA, Harton GL, Palmer FT, Sisson ME, Starr KM, Dennison-Lagos L, Calvo L, Sherins RJ, Bick D, Schulman JD, Black SH: DNA-based X-enriched sperm separation as an adjunct to pre-implantation genetic testing for the prevention of X-linked disease. Hum Reprod 1995;10:979-982
9. Barlow P, Vosa CG: The Y chromosome in human spermatozoa. Nature 1970;226:961-962
10. Sumner AT, Robinson JA, Evans HJ: Distinguishing between X, Y and YY-bearing spermatozoa by fluorescence and DNA content. Nature 1971;229:231-233
11. Pearson PL, Bobrow M, Vosa CG: Technique for identifying Y chromosome in human interphase nuclei. Nature 1970;226:78-80
12. Thomsen JL, Niebuhr E: The frequency of false-positive and false-negative results in the detection of Y-chromosomes in interphase nuclei. Hum Genet 1986;73:27-30
13. van Kooij RJ, van Oost BA: Determination of sex ratio of spermatozoa with a deoxyribonucleic acid-probe and quinacrine staining: a comparison. Fertil Steril 1992;58:384-386
14. Wang H-X, Flaherty SP, Swann NJ, Matthews CD: Discontinuous Percoll gradients enrich X-bearing human spermatozoa: A study using double-label fluorescence in-situ hybridization. Hum Reprod 1994;9:1265-1270
15. Tohyama Y, Oshio S, Yotsukura M, Umeda T, Mohri H: Percoll density gradients centrifugation can separate human X-bearing sperm. J Assist Reprod Genet 1997;14 (Suppl):194s
16. Martini E, Speel EJ, Geraedts JP, Ramaekers FC, Hopman AH: Application of different in-situ hybridization detection methods for human sperm analysis. Hum Reprod 1995;10:855-861.
17. Gledhill BL: Selection and separation of X- and Y-chromosome-bearing mammalian sperm. Gamete Res 1988;20:377-395.