Sperm kinetics and morphology before and after fractionation on discontinuous percoll gradient for sex preselection: Computerized analyses

Archives of Andrology

Volumn 37, Issue 1, 1996, Pages 1-5

  Watkins, A M ^a   Chan, P J ^a   Patton, W C ^a   Jacobson, J D ^a   King, A ^a  

^a LOMA LINDA UNIVERSITY   (United States)

Author keywords

CASA; Gender selection; Morphology; Motility; Percoll; Sex preselection; Sperm wash; Spermatozoa

Indexed keywords

ARTICLE; CELL FRACTIONATION; CELL KINETICS; CELL STRUCTURE; COMPUTER ANALYSIS; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; GENDER; GENETIC ENGINEERING; HUMAN; HUMAN CELL; LONGEVITY; PRIORITY JOURNAL; SEMEN ANALYSIS; SEX DIFFERENCE; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL; X CHROMOSOME; Y CHROMOSOME;

EID: 79961236143     PISSN: 01485016     EISSN: None     Source Type: Journal    
DOI: 10.3109/01485019608988494     Document Type: Article

References (8)

1. Batzofin J, Tran C, Tan T, Reyes R, Mansourian M, Nelson J, Serafini P (1995): XY sperm separation for sex selection: experience with a percoll gradient technique in a clinical setting. Proceedings 43rd Pacific Coast Fertil. Soc. Meeting, Coronado Bay, California, No. 41, p a28.
2. Chan PJ, Tredway DR, Su BC, Corselli J, Pang SC (1992): A double method sperm wash for artificial insemination. Arch Androl 29:43-48.
3. Cui K-H, Matthews CD (1993): X larger than Y. Nature 366:117-118.
4. Iizuka R, Kaneko S, Aoki R, Kobayashi T (1987): Sexing of human sperm by discontinuous Percoll density gradient and its clinical application. Hum Reprod 2:573-575.
5. Kaneko S, Yamaguchi J, Kobayashi T, Iizuka R (1983): Separation of human X- and Y-bearing sperm using percoll density gradient centrifugation. Fertil Steril 40:661-665.
6. Shettles LB (1973): Sperm morphology, cervical milieu, time of insemination and sex ratios. Andrologie 5:227-230.
7. Shettles LB (1990): How sperm commence movement and their isolation for in vitro fertilization and sex selection. Am J Obstet Gynecol 163:270.
8. Wang H-X, Flaherty SP, Swann NJ, Matthews CD (1994): Discontinuous percoll gradients enrich X-bearing human spermatozoa: a study using double-label fluorescence in-situ hybridization. Hum Reprod 9:1265-1270.