Mechanism by which calcium phosphate coprecipitation enhances adenovirus-mediated gene transfer

Gene Therapy

Volumn 6, Issue 11, 1999, Pages 1845-1850

  Walters, R W ^a   Welsh, M J ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Adenovirus; Airway; Calcium phosphate; Cystic fibrosis; Gene transfer

Indexed keywords

CALCIUM PHOSPHATE; INTEGRIN; VIRUS VECTOR;

ADENOVIRUS; ARTICLE; CONTROLLED STUDY; ENDOCYTOSIS; GENE TRANSFER; HUMAN; HUMAN CELL; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; RESPIRATORY EPITHELIUM; VIRUS CELL INTERACTION; VIRUS INFECTION;

ADENOVIRIDAE; CALCIUM PHOSPHATES; CELLS, CULTURED; CYSTIC FIBROSIS; EPITHELIAL CELLS; GENE TRANSFER TECHNIQUES; HUMANS;

ADENOVIRIDAE; RNA VIRUSES;

EID: 0032708738     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301020     Document Type: Article

References (28)

1. O'Neal WK, Beaudet AL. Somatic gene therapy for cystic fibrosis. Hum Mol Genet 1994; 3: 1497-1502.
2. Crystal RG. Transfer of genes to humans: early lessons and obstacles to success. Science 1995; 270: 404-410.
3. Welsh MJ, Tsui L-C, Boat TF, Beaudet AL. Cystic fibrosis. In: Scriver CR, Beaudet AL, Sly WS, Valle D (eds). The Metabolic and Molecular Basis of Inherited Disease. McGraw-Hill: New York, 1995, pp 3799-3876.
4. Grubb BR et al. Inefficient gene transfer by adenovirus vector to cystic fibrosis airway epithelia of mice and humans. Nature 1994; 371: 802-806.
5. Zabner J, Zeiher BG, Friedman E, Welsh MJ. Adenovirus-mediated gene transfer to ciliated airway epithelia requires prolonged incubation time. J Virol 1996; 70: 6994-7003.
6. Zabner J et al. Lack of high affinity fiber receptor activity explains the resistance of ciliated airway epithelia to adenovirus infection. J Clin Invest 1997; 100: 1144-1149.
7. Pickles RJ et al. Limited entry of adenovirus vectors into well-differentiated airway epithelium is responsible for inefficient gene transfer. J Virol 1998; 72: 6014-6023.
8. Walters RW et al. Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia. J Biol Chem 1999; 274: 10219-10226.
9. Bergelson JM et al. Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5. Science 1997; 275: 1320-1323.
10. Wickham TJ, Mathias P, Cheresh DA, Nemerow GR. Integrins alpha beta 3 and alpha beta 5 promote adenovirus internalization but not virus attachment. Cell 1993; 73: 309-319.
11. Greber UF, Willetts M, Webster P, Helenius A. Stepwise dismantling of adenovirus 2 during entry into cells. Cell 1993; 75: 477-486.
12. Mathias P, Wickham T, Moore M, Nemerow G. Multiple adenovirus serotypes use alpha integrins for infection. J Virol 1994; 68: 6811-6814.
13. Fasbender A et al. Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo. J Clin Invest 1998; 102: 184-193.
14. Leopold PL et al. Fluorescent virions: dynamic tracking of the pathway of adenoviral gene transfer vectors in living cells. Hum Gene Ther 1998; 9: 367-378.
15. Lee JH, Zabner J, Welsh MJ. Delivery of an adenovirus vector in a calcium phosphate coprecipitate enhances the therapeutic index of gene transfer to airway epithelia. Hum Gene Ther 1999; 10: 603-613.
16. Bai M, Harfe B, Freimuth P. Mutations that alter an Arg-Gly-Asp (RGD) sequence in the adenovirus type 2 penton base protein abolish its cell-rounding activity and delay virus reproduction in flat cells. J Virol 1993; 67: 5198-5205.
17. Persson R, Svensson U, Everitt E. Virus receptor interaction in the adenovirus system. ii. capping and cooperative binding of virions on hela cells. J Virol 1983; 46: 956-963.
18. Greber UF, Singh I, Helenius A. Mechanisms of virus uncoating. Trends Microbiol 1994; 2: 52-56.
19. Wickham TJ, Filardo EJ, Cheresh DA, Nemerow GR. Integrin alpha beta 5 selectively promotes adenovirus mediated cell membrane permeabilixation. J Cell Biol 1994; 127: 257-264.
20. Seth P, Willingham MC, Pastan I. Adenovirus-dependent release of 51 cr from kb cells at an acidic ph. J Biol Chem 1984; 259: 14350-14353.
21. Svensson U. Role of vesicles during adenovirus 2 internalization into hela cells. J Virol 1985; 55: 442-449.
22. Greber UF et al. The role of the nuclear pore complex in adenovirus DNA entry. EMBO J 1997; 16: 5998-6007.
23. Kondo M, Finkbeiner WE, Widdicombe JH. Simple technique for culture of highly differentiated cells from dog tracheal epithelium. Am J Physiol 1991; 261: L106-L117.
24. Yamaya M, Finkbeiner WE, Chun SY, Widdicombe JH. Differentiated structure and function of cultures from human tracheal epithelium. Am J Physiol 1992; 262: L713-L724.
25. Welsh MJ et al. Adenovirus-mediated gene transfer for cystic fibrosis: Part A. Safety of dose and repeat administration in the nasal epithelium. Part B. Clinical efficacy in the maxillary sinus. Hum Gene Ther 1995; 6: 205-218.
26. Flotte TR et al. A fluorescence video-endoscopy technique for detection of gene transfer and expression. Gene Therapy 1998; 5: 166-173.
27. Zolotukhin S et al. A 'humanized' green fluorescent protein cDNA adapted for high-level expression in mammalian cells. J Virol 1996; 70: 4646-4654.
28. Zabner J et al. A chimeric type 2 adenovirus vector with a type 17 fiber enhances gene transfer to human airway epithelia. J Virol (in press).