cDNA cloning and characterization of two Dendranthema x morifolium anthocyanin malonyltransferases with different functional activities

Plant Science

Volumn 166, Issue 1, 2004, Pages 89-96

  Suzuki, Hirokazu ^a   Nakayama, Toru ^a   Yamaguchi, Masa Atsu ^b   Nishino, Tokuzo ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b MINAMI KYUSHU UNIVERSITY   (Japan)

Author keywords

Anthocyanin; Chrysanthemum; Compositae; Dendranthema x morifolium; Malonyltransferase; VAT family; Versatile acyltransferase family

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; CELLS; CLONING; DNA; ENZYMES; ESCHERICHIA COLI;

MALONYLATION;

PLANTS (BOTANY);

ASTERACEAE; CHRYSANTHEMUM; CHRYSANTHEMUM X MORIFOLIUM; DAHLIA; DAHLIA VARIABILIS; DENDRANTHEMA MORIFOLIUM; ESCHERICHIA COLI; PERICALLIS CRUENTA; SENECIO; SENECIO CRUENTUS;

EID: 1642526751     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plantsci.2003.08.010     Document Type: Article

References (15)

1. D. Strack, V. Wray, The anthocyanins, in: J.B. Harbone (Ed.), The Flavonoids, Chapman & Hall/CRC, Washington, DC, 1994, pp. 1-22.
2. Harbone J.B., Williams C.A. Advances in flavonoid research since 1992. Phytochemistry. 55:2000;481-504.
3. Honda T., Saito N. Recent progress in the chemistry of polyacylated anthocyanins as flower color pigments. Heterocycles. 56:2002;633-692.
4. Goto T., Kondo T. Structure and molecular stacking of anthocyanins: Flower color variation. Angew Chem. Int. Ed. Engl. 30:1991;17-33.
5. Suzuki H., Nakayama T., Yonekura-Sakakibara K., Fukui Y., Nakamura N., Yamaguchi M.-A., Tanaka Y., Kusumi T., Nishino T. cDNA cloning, heterologous expressions, and functional characterization of malonyl-coenzyme A: anthocyanidin 3-O-glucoside-6″-O-malonyltransferase from dahlia flowers. Plant Physiol. 130:2002;2142-2151.
6. Yoshimoto M., Okuno S., Yamaguchi M.-A., Yamakawa O. Antimutagenicity of deacylated anthocyanins in purple-fleshed sweet potato. Biosci. Biotech. Biochem. 65:2001;1652-1655.
7. Nakayama T., Suzuki H., Nishino T. Anthocyanin acyltransferases: specificities, mechanism, phylogenetics, and applications. J. Mol. Catal. B: Enzym. 23:2003;117-132.
8. Fujiwara H., Tanaka Y., Yonekura-Sakakibara K., Fukuchi-Mizutani M., Nakao M., Fukui Y., Yamaguchi M.-A., Ashikari T., Kusumi T. cDNA cloning, gene expression, and subcellular localization of anthocyanin 5-aromatic acyltransferase from Gentiana triflora. Plant J. 16:1998;421-431.
9. Yonekura-Sakakibara K., Tanaka Y., Fukuchi-Mizutani M., Fujiwara H., Fukui Y., Ashikari T., Murakami Y., Yamaguchi M.-A., Kusumi T. Molecular and biochemical characterization of a novel hydroxycinnamoyl-CoA:anthocyanin 3-O-glucoside-6″-O-acyltransferase from Perilla frutescens. Plant Cell. Physiol. 41:2000;495-502.
10. Suzuki H., Nakayama T., Yonekura-Sakakibara K., Fukui Y., Nakamura N., Nakao M., Tanaka Y., Yamaguchi M.-A., Kusumi T., Nishino T. Malonyl-CoA: anthocyanin 5-O-glucoside-6″′-O-malonyltransferase from scarlet sage (Salvia splendens) flowers. J. Biol. Chem. 276:2001;49013-49019.
11. Suzuki H., Sawada S., Yonekura-Sakakibara K., Nakayama T., Yamaguchi M.-A., Nishino T. Identification of a cDNA encoding malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6(-O-malonyltransferase from cineraria (Senecio cruentus) flowers. Plant Biotech. 20:2003;229-234.
12. Nakayama M., Koshioka M., Shibata M., Hiradate S., Sugie H., Yamaguchi M.-A. Identification of cyanidin 3-O-(3″,6″-O-dimalonyl-β- glucopyranoside) as a flower pigment of chrysanthemum (Dendranthema grandiflorum). Biosci. Biotech. Biochem. 61:1997;1607-1608.
13. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
14. St-Pierre B., Laflamme P., Alarco A.-M., De Luca V. The terminal O-acetyltransferase involved in vindoline biosynthesis defines a new class of proteins responsible for coenzyme A-dependent acyl transfer. Plant J. 14:1998;703-713.
15. Suzuki H., Nakayama T., Nishino T. Proposed mechanism and functional amino acid residues of malonyl-CoA:anthocyanin 5-O-glucoside-6″′-O- malonyltransferase from flowers of Salvia splendens, a member of the versatile plant acyltransferase family. Biochemistry. 42:2003;1764-1771.