Comparison of the Effect of Mitochondrial Inhibitors on Mitochondrial Membrane Potential in Two Different Cell Lines Using Flow Cytometry and Spectrofluorometry

Cytometry Part A

Volumn 52, Issue 2, 2003, Pages 110-116

  Kalbáčová, Marie ^a   Vrbacký, Marek ^a   Drahota, Zdeněk ^b   Mělková, Zora ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

^b INSTITUTE OF PHYSIOLOGY   (Czech Republic)

Author keywords

Flow cytometry; Mitochondrial inhibitors; Mitochondrial membrane potential; Oxygen consumption; Spectrofluorometry

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANTIMYCIN A1; ATRACTYLOSIDE; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; OLIGOMYCIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLOW CYTOMETRY; HELA CELL; HUMAN; HUMAN CELL; KIDNEY CELL; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; PRIORITY JOURNAL; SPECTROFLUOROMETRY;

EID: 1642474354     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10031     Document Type: Article

References (17)

1. Chen LB. Mitochondrial membrane potential in living cells. Annu Rev Cell Dev Biol 1988;4:155-181.
2. Voet D, Voet JG. Biochemistry. 2nd ed. New York: John Wiley & Sons; 1995. p 549.
3. Schlattner U, Dolder M, Wallimann T, Tokarska-Schlattner M. Mitochondrial creatine kinase and mitochondrial outer membrane porin show a direct interaction that is modulated by calcium. J Biol Chem 2001;276:48027-48030.
4. Fiore C, Trezeguet V, Le Saux A, Roux P, Schwimmer C, Dianoux AC, Noel F, Lauquin GJ, Brandolin G, Vignais PV. The mitochondrial ADP/ATP carrier: structural, physiological and pathological aspects. Biochimie 1998;80:137-150.
5. Moreira ME, Sorensen AC, Heard KJ, Harken AH, Banerjee A, Cairns CB. Reduction in the mitochondrial energy potential by inhibition of electron transport in human vascular smooth muscle cells. Acad Emerg Med 2001;8:548.
6. Macouillard-Poulletier de G, Belaud-Rotureau MA, Volsin P, Leducq N, Belloc F, Canioni P, Diolez P. Flow cytometric analysis of mitochondrial activity in situ: application to acetylceramide-induced mitochondrial swelling and apoptosis. Cytometry 1998;33:333-339.
7. Khaled AR, Reynolds DA, Young HA, Thompson CB, Muegge K, Durum SK. Interleukin-3 withdrawal induces an early increase in mitochondrial membrane potential unrelated to the Bcl-2 family. Roles of intracellular pH, ADP transport, and F(0)F(1)-ATPase. J Biol Chem 2001;276:6453-6462.
8. Skowronek P, Haferkamp O, Rodel G. A fluorescence-microscopic and flow-cytometric study of HeLa cells with an experimentally induced respiratory deficiency. Biochem Biophys Res Commun 1992;187:991-998.
9. Kalbacova M, Vrbacky M, Humlova Z, Melkova Z. Protooncogene Bcl-2 induces apoptosis in several cell lines. Folia Biol (Praha) 2002;48:15-27.
10. Bunting JR, Phan TV, Kamali E, Dowben RM. Fluorescent cationic probes of mitochondria. Metrics and mechanism of interaction. Biophys J 1989;56:979-993.
11. Poot M, Pierce RH. Analysis of mitochondria by flow cytometry. Methods Cell Biol 2001;64:117-128.
12. Shapiro HM. Cell membrane potential analysis. Methods Cell Biol 1990;33:25-35.
13. Gnaiger E, Steinlechner-Maran R, Mendez G, Eberl T, Margreiter R. Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 1995;27:583-596.
14. Pedersen PL. Tumor mitochondria and the bioenergetics of cancer cells. Prog Exp Tumor Res 1978;22:190-274.
15. Vrbacky M, Krijt J, Drahota Z, Mělková Z. Inhibitory Effects of Bcl-2 on Mitochondrial Respiration. Physiological Research 2003 (in press).
16. Bernardi P, Scorrano L, Colonna R, Petronilli V, Di Lisa F. Mitochondria and cell death. Mechanistic aspects and methodological issues. Eur J Biochem 1999;264:687-701.
17. Kroemer G, Petit P, Zamzami N, Vayssiere JL, Mignotte B. The biochemistry of programmed cell death. FASEB J 1995;9:1277-1287.