Analysis of mitochondria by flow cytometry

Methods in Cell Biology

Volumn 64, Issue 64, 2001, Pages 117-128

  Poot, Martin ^a   Pierce, Robert H ^b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b WRIGHT PATTERSON AIR FORCE BASE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARDIOLIPIN; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; PHOTOPROTEIN;

ANIMAL; ARTICLE; CELL MEMBRANE POTENTIAL; FLOW CYTOMETRY; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MITOCHONDRION; OXIDATION REDUCTION REACTION; STAINING; TISSUE FIXATION;

ANIMALS; CARDIOLIPINS; FLOW CYTOMETRY; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MEMBRANE POTENTIALS; MITOCHONDRIA; NAD; OXIDATION-REDUCTION; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING; TISSUE FIXATION;

EID: 35449002132     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)64009-3     Document Type: Article

References (32)

1. Backway, K. L., McCulloch, E. A., Chow, S., and Hedley, D. W. (1997). Relationships between the mitochondrial permeability transition and oxidative stress during ara-C toxicity. Cancer Res. 57, 2446-2451.
2. Chen, L. B. (1989). Fluorescent labeling of mitochondria. Methods Cell Biol. 29, 103-123.
3. Cossarizza, A., Kalashnikova, G., Grassilli, E., Chiapelli, F., Salvioli, S., Capri, M., Barbieri, D., Troiano, L., Monti, D., and Franceschi, C. (1994). Mitochondrial modifications during rat thymocyte apoptosis: A study at the single cell level. Exp. Cell Res. 214, 323-330.
4. Gallet, P. F., Maftah, A., Petit, J. M., Denis-Gay, M., and Julien, R. (1995). Direct cardiolipin assay in yeast using red fluorescence emission of 10-N-nonyl acridine orange. Eur. J. Biochem. 228, 113-119.
5. Gilmore, K., and Wilson, M. (1999). The use of chloromethyl-X-rosamine (Mito Tracker Red) to measure loss of mitochondrial membrane potential in apoptotic cells is incompatible with cell fixation. Cytometry 36, 355-358.
6. Green, D. R., and Reed, J. C. (1998). Mitochondria and apoptosis. Science 281, 1309-1312.
7. Hollinshead, M., Sanderson, J., and Vaux, D. J. (1997). Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin. J. Histochem. Cytochem. 45, 1053-1057.
8. Johnson, L. V., Walsh, M. L., Bockus, B. J., and Chen, L. B. (1981). Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy. J. Cell Biol. 88, 526-535.
9. Kreen, M., Farinas, J., Li, Y., and Verkman, A. S. (1998). Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74, 1591-1599.
10. Kendall, J. M., Dormer, R. L., and Campbell, A. K. (1992). Targeting aequorin to the endoplasmic reticulum of living cells. Biochem. Biophys. Res. Commun. 189, 1008-1016.
11. Kroemer, G., Dallaporta, B., and Resche-Rigon, M. (1998). The mitochondrial death/life regulator in apoptosis and necrosis. Annu. Rev. Physiol. 60, 619-642.
12. Llopis, J., McCaffery, M., Miyawaki, A., Farquhar, M. G., and Tsien, R. Y. (1998). Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 95, 6803-6808.
13. Macho, A., Decaudin, D., Castedo, M., Hirsch, T., Susin, S. A., Zamzami, N., and Kroemer, G. (1996). Chloromethyl-X-Rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis. Cytomelry 25, 333-340.
14. Maftah, A., Petit, J. M., Ratinaud, M. H., and Julien, R. (1989). 10 N-Nonyl-acridine orange: A fluorescent probe which stains mitochondria independently of their energetic state. Biochem. Biophys. Res. Commun. 164, 185-190.
15. M'etivier, D., Dallaporta, B., Zamzami, N., Larochette, N., Susin, S. A., Marzo, I., and Kroemer, G. (1998). Cytofluorometric detection of mitochondrial alterations in early CD95/Fas/APO-1-triggered apoptosis of Jurkat T lymphoma cells. Comparison of seven mitochondrion-specific fluorochromes. Immunol. Lett. 61, 157-163.
16. Petit, J.-M., Maftah, A., Ratinaud, M.-H., and Julien, R. (1992). 10 N-Nonyl-acidine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria. Eur. J. Biochem. 209, 267-273.
17. Pierce, R. H., Campbell, J. S., Stephenson, A. B., Franklin, C. C., Chaisson, M., Poot, M., Kavanagh, T. J., and Fausto, N. (2000). Disruption of redox homeostasis in tumor necrosis factor induced apoptosis in murine hepatocytes. Am. J. Pathol. 157, 221-236.
18. Poot, M., and Pierce, R. H. (1999). Detection of changes in mitochondrial function during apoptosis by simultaneous staining with multiple fluorescent dyes and correlated multiparameter flow cytometry. Cytometry 35, 311-317
19. Poot, M., Kavanagh, T. J., Kang, H.-C., Haugland, R. P., Rabinovitch, P. S. (1991). Flow cytometric analysis of cell cycle-dependent changes in cell thiol level by combining a new laser dye with Hoechst 33342. Cytometry 12, 184-187.
20. Poot, M., Zhang, Y.-Z., Krämer, J., Wells, K. S., Jones, L. J., Hanzel, D. K., Lugade, A. G., Singer, V. L., and Haugland, R. P. (1996). Analysis of mitochondrial morphology and function with novel fixable fluorescent stains. J. Histochem. Cytochem. 44, 1363-1372.
21. Reers, M., Smith, T. W., and Chen, L. B. (1991). J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 30, 4480-4486.
22. 2+ revealed by specifically targeted recombinant aequorin. Nature 358, 325-327.
23. Rizzuto, R., Brini, M., and Pozzan, T. (1994). Targeting recombinant aequorin to specific intracellular organelles. Methods Cell Biol. 40, 339-358.
24. 6(3) or rhodamine 123, is a reliable fluorescent probe to assess Δψ changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411, 77-82.
25. Shapiro, H. M. (1994). Cell membrane potential analysis. Methods Cell Biol. 41, 121-133.
26. Sims, P. J., Waggoner, A. S., Wang, C. H., and Hoffman, J. F. (1974). Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry 13, 3315-3330.
27. Smiley, S. T., Reers, M., Mottola-Hartshorn, C., Lin, M., Chen, A., Smith, T. W., Steele, G. D., Jr., and Chen, L. B. (1991). Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. U.S.A. 88, 3671-3675.
28. Terasaki, M., Song, J., Wong, J. R., Weiss, M. J., and Chen, L. B. (1984). Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 38, 101-108.
29. Thorell, B. (1983). Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levels. Cytometry 4, 61-65.
30. xL regulates the membrane potential and volume homeostasis of mitochondria. Cell 191, 627-637.
31. Wallace, D. C. (1995). Mitochondrial DNA variation in human evolution, degenerative disease, and aging. Am. J. Hum. Genet. 57, 201-223.
32. Whitaker, J. E., Moore, P. L., Haugland, R. L., and Haugland, R. P. (1991). Dihydrotetramethylrosamine: A long wavelength, fluorogenic peroxidase substrate evaluated in vitro and in a model phagocyte. Biochem. Biophys. Res. Commun. 175, 387-393.