Flow cytometric analysis of mitochondrial activity in situ: Application to acetylceramide-induced mitochondrial swelling and apoptosis

Cytometry

Volumn 33, Issue 3, 1998, Pages 333-339

  Macouillard Poulletier De Gannes, Florence ^a   Belaud Rotureau, Marc Antoine ^b   Voisin, Pierre ^a   Leducq, Nathalie ^a   Belloc, Francis ^b   Canioni, Paul ^a   Diolez, Philippe ^a  

^a CNRS University Bordeaux Segalen   (France)

^b HAUT LÉVÊQUE HOSPITAL   (France)

Author keywords

Acetyl ceramide; Apoptosis; Flow cytometry; Light scattering; Microglia; Mitochondrial membrane potential; Mitochondrial volume; U 937

Indexed keywords

APOPTOSIS; ARTICLE; DIAGNOSTIC VALUE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LIGHT SCATTERING; MICROGLIA; MITOCHONDRIAL MEMBRANE; MITOCHONDRION SWELLING; PRIORITY JOURNAL;

ANIMALS; APOPTOSIS; CELL LINE; CELL SURVIVAL; CERAMIDES; FLOW CYTOMETRY; HUMANS; MITOCHONDRIA, LIVER; MITOCHONDRIAL SWELLING; OLIGOMYCINS; OXYGEN CONSUMPTION; POTASSIUM CYANIDE; RATS; RATS, WISTAR; TIME FACTORS; U937 CELLS;

EID: 0032212192     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19981101)33:3<333::AID-CYTO7>3.0.CO;2-H     Document Type: Article

References (23)

1. Beavis AD, Brannan RD. Garlid KD: Swelling and contraction of the mitochondrial matrix. I. A structural interpretation of the relationship between light scattering and matrix volume. J Biol Chem 260:13424-13433, 1985.
2. Belloc F, Dumain P, Boisseau MR, Jaloustre C, Reiffers J, Bernard P, Lacombe F: A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells. Cytometry 17:59-65, 1994.
3. Bradford MM: Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254, 1976.
4. Brand MD, Harper M-E, Taylor HC: Control of the effective P/O ratio of oxidative phosphorylation in liver mitochondria and hepatocytes. Biochem J 291:739-748, 1993.
5. Chance B, Williams GR: Respiratory enzymes in oxidative phosphorylation. J Biol Chem 217:409-427, 1955.
6. Diolez P, Moreau F: Effect of bovine serum albumin on membrane potential in plant mitochondria. Physiol Plant 59:177-182, 1983.
7. Dufour S, Rousse N, Canioni P, Diolez P: Top-down control analysis of temperature effect on oxidative phosphorylation. Biochem J 314: 743-751, 1996.
8. Emaus RK, Grundwald R, Lemasters JJ: Rhodamine 123 as a probe of trasmembrane potential in isolated rat-liver mitochondria: Spectral and metabolic properties. Biochim Biophys Acta 850:436-448, 1986.
9. Estabrook RW: Mitochondrial respiratory control and the polarographic measurement of ADP/O ratios. Methods Enzymol 10:41-47, 1967.
10. Goubern M, Yazbeck J, Chapey M-F, Diolez P, Moreau F: Variations of energization parameters and proton conductance induced by cold adaptation and essential fatty acid deficiency in mitochondria of rat brown adipose tissue. Biochim Biophys Acta 1015:334-340, 1990.
11. Hackenbrock CR, Rehn TG, Weinbach EC, Lemasters JJ: Oxidative phosphorylation and ultrastructural transformation in mitochondria in the intact ascites tumor cell. J Cell Biol 51:123-157, 1971.
12. Halestrap A: The regulation of the matrix volume of mammalian mitochondria in vivo and in vitro and its role in the control of mitochondrial metabolism. Biochim Biophys Acta 973:355-382, 1989.
13. Liu Z, Bushnell WR, Brambl R: Potentiometric cyanine dyes are sensitive probes for mitochondria in intact plant cells. Plant Physiol 84:1385-1390, 1987.
14. Moore AL, Bonner JWD: Measurements of membrane potentials in plant mitochondria with safranine method. Plant Physiol 70:1271-1276, 1982.
15. Nobes CD, Lakin-Thomas PL. Brand MD: The contribution of ATP turnover by the Na+/K+-ATPase to the rate of respiration of hepatocytes. Effects of thyroid status and fatty acids. Biochim Biophys Acta 976:241-245, 1989.
16. Petit P, Diolez P, de Kouchkovsky Y: Flow cytometric analysis of energy transducing organelles: Mitochondria and chloroplasts. In: Flow Cytometry: Advanced Research and Clinical Applications, Yen A (ed). CRC Press, Boca Raton, FL, 1989, pp. 271-303.
17. Petit PX, O'Connor JE, Grundwald D, Brown NC: Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications. Eur J Biochem 194:389-397, 1990.
18. Petit PX, Susin SA, Zamzami N, Mignotte B, Kroemer G: Mitochondria and programmed cell death: Back to future. FEBS Lett 396:7-14, 1996.
19. Quinlan PT, Thomas AP, Armston AE, Halestrap AP: Measurement of the intramitochondrial volume in hepatocytes without cell disruption and its elevation by hormones and valinomycin. Biochem J 214:395-404, 1983.
20. Ronot XL, Benel M, Adolphe M, Mounolou JC: Mitochondrial analysis in living cells: The use of rhodamine 123 and flow cytometry. Biol Cell 57:1, 1986.
21. Smith JC: Potential-sensitive molecular probes in membranes of bioenergetic relevance. Biochim Biophys Acta 1016:1-28, 1990
22. Smyth MJ, Obeid LM, Hannun YA: Ceramide: A novel lipid mediator of apoptosis. Adv Pharmacol 41:133-153, 1997.
23. Susin SA, Zamzami N, Larochette N, Dallaporta B, Marzo I, Brenner C, Hirsch T, Petit PX, Geuskens M, Kroemer G: A cytofluorometric assay of nuclear apoptosis induced in a cell free system: Application to ceramide-induced apoptosis. Exp Cell Res 236:397-403, 1997.