Detection of streptomycetes in house dust - Comparison of culture and PCR methods

Indoor Air

Volumn 14, Issue 2, 2004, Pages 112-119

  Rintala, H ^a   Hyvarinen A ^a   Paulin, L ^b   Nevalainen, A ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Dust; Measuring methods; Microbe; Moisture damage; Polymerase chain reaction; Streptomyces

Indexed keywords

BACTERIA; CHAINS; DAMAGE DETECTION; DUST; FUNGI; HOUSES; METABOLITES; MOISTURE; RNA;

%MOISTURE; DUST SAMPLES; GRAM POSITIVE; HOUSE DUST; MEASURING METHOD; MICROBE; MOISTURE DAMAGE; POLYMERASE CHAIN REACTION METHODS; STREPTOMYCES; STREPTOMYCETES;

POLYMERASE CHAIN REACTION;

GLUCOSE; RNA 16S; BACTERIAL DNA; WATER;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; FISHER EXACT TEST; HOUSE DUST; MOISTURE; NONHUMAN; POLYMERASE CHAIN REACTION; RANK SUM TEST; SAMPLING; STATISTICAL ANALYSIS; STREPTOMYCES; BACTERIAL SPORE; COMPARATIVE STUDY; EVALUATION; GENETICS; HOUSING; INDOOR AIR POLLUTION; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION;

MARTES PENNANTI; POSIBACTERIA; STREPTOMYCES; STREPTOMYCINEAE; UNCULTURED ACTINOMYCETE;

AIR POLLUTION, INDOOR; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; HOUSING; POLYMERASE CHAIN REACTION; SPORES, BACTERIAL; STREPTOMYCES; WATER;

EID: 1642444027     PISSN: 09056947     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1600-0668.2003.00219.x     Document Type: Article

References (38)

1. Andersson, A.M., Weiss, N., Rainey, F. and Salkinoja-Salonen, M.S. (1999) Dustborne bacteria in animal sheds, schools and children's day care centres. J. Appl. Microbiol., 86, 622-634.
2. Beguin, H. and Nolard, N. (1996) Prevalence of fungi in carpeted floor environment: analysis of dust samples from livingrooms, bedrooms, offices and school classrooms. Aerobiologia, 12, 113-120.
3. Bornehag, C.-G., Blomquist, G., Gyntelberg, F., Järvholm, B., Malmberg, P., Nordvall, L., Nielsen, A., Pershagen, G. and Sundell, J. (2001) Dampness in buildings and health. Indoor Air 11, 72-96.
4. Braun-Fahrländer, C., Riedler, J., Herz, U., Eder, W., Waser, M., Grize, L., Maish S., Carr, D., Gerlach, F., Bufe, A., Lauener, R.P., Schierl, R., Renz, H., Nowak, D. and von Mutius, E. (2002) Environmental exposure to endotoxin and its relation to asthma in school-age children. N. Engl. J. Med., 347, 869-877.
5. Buttner, M.P., Cruz-Perez, P. and Stetzenbach, L.D. (2001) Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR. Appl. Environ. Microbiol., 67, 2564-2570.
6. Cruz-Perez, P., Buttner, M.P. and Stetzenbach, L.D. (2001) Detection and quantitation of Aspergillus fumigatus in pure culture using polymerase chain reaction. Mol. Cell. Probes., 15, 81-88.
7. Dales, R.E., Miller, D. and McMullen, E.D. (1997) Indoor air quality and health: validity and determinants of reported home dampness and moulds. Int. J. Epidemiol., 26, 120-125.
8. Demain, A.L. (1999) Pharmaceutically active secondary metabolites of microorganisms. Appl. Microbiol. Biotechnol., 52, 455-463.
9. Edwards, U., Rogall, T., Blöcker, H., Emde, M. and Böttger, E.C. (1989) Isolation and direct complete nucleotide determination of entire genes. Characterisation of a gene coging for 16S ribosomal RNA. Nucleic. Acids. Res., 17, 7843-7853.
10. Felsenstein, J. (1989) PHYLIP-phylogeny interference package (version 3.2). Cladistics, 5, 164-166.
11. Flannigan, B. (1997) Air sampling for fungi in indoor environments. J. Aerosol Sci., 28, 381-392.
12. Flannigan, B., Vicars, S., Pasanen, A. and Pasanen, P. (1994) Bioaerosols from housedust. In: Samson, R.A., Flannigan, B., Verhoeff, A.P., Adan, O.C.G and Hoekstra, E.S. Health Implications of Fungi in Indoor Air Environments, Amsterdam, Elsevier Science, pp. 529-538.
13. Gerber, N.N. and Lechevalier, H.A. (1965) Geosmin, an earthy-smelling substance isolated from actinomycetes. Appl. Microbiol., 13, 935-938.
14. Goodfellow, M. and Simpson, K.E. (1987) Ecology of streptomycetes. Frontiers Appl. Microbiol., 2, 97-125.
15. Hameed, A., Awad, A. and Farag, S.A. (1999) An indoor bio-contaminants air quality. Int. J. Environ. Health Res., 9, 313-319.
16. Haugland, R.A., Vesper, S.J. and Wymer, L.J. (1999) Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqMan™ fluorogenic probe system. Mol. Cell. Probes. 13, 329-340.
17. Haverinen, U., Husman, T., Vahteristo, M., Koskinen, O., Moschandreas, D., Nevalainen, A. and Pekkanen, J. (2001) Comparison of two-level and three-level classifications of moisture-damaged dwellings in relation to health effects. Indoor Air, 11, 192-199.
18. Hirvonen, M.-R., Ruotsalainen, M., Savolainen, K. and Nevalainen, A. (1997) Effect of viability of actinomycete spores on their ability to stimulate production of nitric oxide and reactive oxygen species in RAW264.7 macrophages. Toxicology, 124, 105-114.
19. Hyvärinen, A., Meklin, T., Vepsäläinen, A. and Nevalainen, A. (2002) Fungi and actinobacteria in moisture-damaged building materials - concentrations and diversity. Int. Biodeter. Biodegrad., 49, 27-37.
20. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16, 111-120.
21. Kuske, C.R., Banton, K.L., Adorada, D.L., Stark, P.C., Hill, K.K. and Jackson, P.J. (1998) Small scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil. Appl. Environ. Microbiol., 64, 2463-2472.
22. Macher, J.M. (2001) Evaluation of a procedure to isolate culturable microorganisms from carpet dust. Indoor Air, 11, 134-140.
23. Murtoniemi, T., Nevalainen, A., Suutari, M., Toivola, M., Komulainen, H. and Hirvonen, M.-R. (2001) Induction of cytotoxicity and production of inflammatory mediators in RAW264.7 macrophages by spores grown on six different plasterboards. Inhal. Toxicol., 13, 233-247.
24. Nevalainen, A., Pasanen, A.-L., Niininen, M., Reponen, T., Kalliokoski, P. and Jantunen, M.J. (1991) The indoor air quality in Finnish homes with mold problems. Environ. Int. 17, 299-302.
25. Nevalainen, A., Partanen, P., Jääskeläinen, E., Hyvärinen, A., Koskinen, O., Meklin, T., Vahteristo, M., Koivisto, J. and Husman, T. (1998) Prevalence of moisture problems in finnish houses. Indoor Air, 4, 45-49.
26. Rintala, H., Nevalainen, A., Rönkä, E. and Suutari, M. (2001) PCR primers targeting the 16S rRNA gene for the specific detection of streptomycetes, Mol. Cell. Probes, 15, 337-347.
27. Roe, J.D., Haugland, R.A., Vesper, S.J. and Wymer, L.J. (2001) Quantification of Stachybotrus chartarum conidia in indoor dust using real time, fluorescent probe-based detection of PCR products. J. Expo. Anal. Environ. Epidemiol., 11, 1-9.
28. Saitou, N. and Nei, M. (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4, 406-425.
29. Samson, R., Flannigan, B., Flannigan, M., Verhoeff, A., Adan, O. and Hoekstra, E. (1994) Recommendations. In: Health Implications of Fungi in Indoor Air Environments, Amsterdam, Elsevier Science, pp. 529-538.
30. Smedje, G. and Norbäck, D. (2001) Irritants and allergens at school in relation to furnishing and cleaning. Indoor Air, 11, 127-133.
31. Stackebrandt, E., Rainey, F.A. and Ward-Rainey, N.L. (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int. J. Syst. Bacteriol., 47, 479-491.
32. Sunesson, A.-L., Nilsson, C.-A., Carlson, R., Blomquist, G. and Andersson, B. (1997) Production of volatile metabolites from Streptomyces albidoflavus cultivated on gypsum board and tryptone glucose extract agar-influence of temperature, oxygen and carbon dioxide levels. Ann. Occup. Hyg., 41, 393-413.
33. Toivola, M., Aim, S., Reponen, T., Kolari, S. and Nevalainen, A. (2002) Personal exposures and microenvironmental concentrations of particles and bioaerosols. J. Environ. Monit., 4, 166-174.
34. Wickman, M., Gravesen, S., Nordvall, S.L., Pershagen, G. and Sundell, J. (1992) Indoor viable dust-bound microfungi in relation to residential characteristics, living habits, and symptoms in atopic and control children. J. Allergy Clin. Immunol., 89, 752-759.
35. Williams, S.T., Goodfellow, M., Alderson, G., Wellington, E.M.H., Sneath, P.H.A. and Sackin, M.J. (1983) Numerical classification of Streptomyces and related genera. J. Gen. Microbiol., 129, 1743-1813.
36. AL. In: Williams, S.T., Sharpe, M.E. and Holt, J.G. (eds) Bergey's Manual of Systematic Bacteriology, Vol. 4, Baltimore, Williams & Wilkins, 2452-2492.
37. von Wintzingerode, F., Göbel, U.B. and Stackebrandt, E. (1997) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS. Microbiol. Rev., 21, 213-229.
38. Zhou, G., Whong, W.-Z., Ong, T. and Chen, B. (2000) Development of a fungus-specific PCR assay foe detecting low-level fungi in an indoor environment. Mol. Cell. Probes., 14, 339-348.