Analysis of proteins copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: Comparison with affinity-tag based protein detection and evaluation of different solubilization methods

Journal of the American Society for Mass Spectrometry

Volumn 15, Issue 4, 2004, Pages 558-567

  Bernhard, Oliver K ^a,b   Cunningham, Anthony L ^a   Sheil, Margaret M ^b  

^a UNIVERSITY OF SYDNEY   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; GELS; MASS SPECTROMETRY; PURIFICATION;

IN-GEL DIGESTS; PROTEIN COMPLEXES;

PROTEINS;

CD4 ANTIGEN; PROTEIN KINASE LCK;

MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANTIGEN PURIFICATION; ARTICLE; CELL LINE; INTERMETHOD COMPARISON; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; SOLUBILIZATION; UNINDEXED SEQUENCE;

AMINO ACID SEQUENCE; ANTIGENS, CD4; BUFFERS; CELL LINE; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LYMPHOCYTE SPECIFIC PROTEIN TYROSINE KINASE P56(LCK); MACROMOLECULAR SUBSTANCES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEINS; REPRODUCIBILITY OF RESULTS; ROSANILINE DYES; SOLUBILITY;

EID: 1642421262     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2003.12.006     Document Type: Article

References (23)

1. Patterson S.D., Aebersold R.H. Proteomics The First Decade and Beyond. Nat. Gen. Suppl. 33:2003;311-323.
2. Ranish J.A., Yi E.C., Leslie D.M., Purvine S.O., Goodlet D.R., Eng J., Aebersold R. The Study of Macromolecular Complexes by Quantitative Proteomics. Nat. Gen. 33:2003;349-355.
3. Ho Y., Gruhler A., Heilbut A., Bader G.D., Moore L., Adams S.L., Millar A., Taylor P., Bennett K., Boutilier K., Yang L., Wolting C., Donaldson I., Schandorff S., Shewnarane J., Vo M., Taggart J., Goudreault M., Muskat B., Alfarano C., Dewar D., Lin Z., Michalickova K., Willems A.R., Sassi H., Nielsen P.A., Rasmussen K.J., Andersen J.R., Johansen L.E., Hansen L.H., Jespersen H., Podtelejnikov A., Nielsen E., Crawford J., Poulsen V., Sorensen B.D., Matthiesen J., Hendrickson R.C., Gleeson F., Pawson T., Moran M.F., Durocher D., Mann M., Hogue C.W., Figeys D., Tyers M. Systematic Identification of Protein Complexes in Saccharomyces cerevisiae. Nature. 415:2002;180-183.
4. Beyers A.D., Spruyt L.L., Williams A.F. Molecular Associations Between the T-lymphocyte Antigen Receptor Complex and the Surface Antigens CD2, CD4, or CD8, and CD5. Proc. Natl. Acad. Sci. U.S.A. 89:1992;2945-2949.
5. Wine R.N., Dial J.M., Tomer K.B., Borchers C.H. Identifications of Components of Protein Complexes Using a Fluorescent Photo-Cross-Linker and Mass Spectrometry. Anal. Chem. 74:2002;1939-1945.
6. Link A.J., Eng J., Schieltz D.M., Carmack E., Mize G.J., Morris D.R., Garvik B.M., Yates J.R. III. Direct Analysis of Protein Complexes Using Mass Spectrometry. Nat. Biotechnol. 17:1999;676-682.
7. Bernhard O.K., Burgess J.A., Hochgrebe T., Sheil M.M., Cunningham A.L. Mass Spectrometry Analysis of CD4 Associated Proteins Using Affinity Chromatography and Affinity Tag-Mediated Purification of Tryptic Peptides. Proteomics. 3:2003;139-146.
8. Spahr C.S., Susin S.A., Bures E.J., Robinson J.H., Davis M.T., McGinley M.D., Kroemer G., Patterson S.D. Simplification of Complex Peptide Mixtures for Proteomic Analysis Reversible Biotinylation of Cysteinyl Peptides. Electrophoresis. 21:2000;1635-1650.
9. Gay D., Maddon P., Sekaly R., Tale M.A., Godfrey M., Long E., Goldstein G., Ches L., Axel R., Kappler J., Marrack P. Functional Interaction Between Human T-Cell Protein CD4 and the Major Histocompatibility Complex HLA-DR Antigen. Nature. 328:1987;626-629.
10. Wu L., Gerard N.P., Wyatt R., Choe H., Parolin C., Ruffing N., Borsetti A., Cardoso A.A., Desjardin E., Newman W., Gerard C., Sodroski J. CD4-Induced Interaction of Primary HIV-1 gp120 Glycoproteins with the Chemokine Receptor CCR-5. Nature. 384:1996;179-183.
11. Lapham C.K., Ouyang J., Chandrasekhar B., Nguyen N.Y., Dimitrov D.S., Golding H. Evidence for Cell-Surface Associations Between Fusin and the CD4-gp120 Complex in Human Cell Lines. Science. 274:1996;602-605.
12. Veillette A., Bookman M.A., Horak E.M., Bolen J.B. The CD4 and CD8 T Cell Surface Antigens are Associated with the Internal Membrane Tyrosine-Protein Kinase p56lck. Cell. 55:1988;301-308.
13. Sheng M., Cummings J., Roldan L.A., Jan Y.N., Jan L.Y. Changing Subunit Composition of Heteromeric NMDA Receptors During Development of Rat Cortex. Nature. 368:1994;144-147.
14. Lynch G.W., Sloane A.J., Raso V., Lai A., Cunningham A.L. Direct Evidence for Native CD4 Oligomers in Lymphoid and Monocytoid Cells. Eur. J. Immunol. 29:1999;2590-2602.
15. Loo J.A., Ogorzalek-Loo R.R. Applying Charge Discrimination with Electrospray Ionisation-Mass Spectrometry to Protein Analyses. J. Am. Soc. Mass Spectrom. 6:1995;1098-1104.
16. Cruikshank W.W., Center D.M., Nisar N., Wu M., Natke B., Theodore A.C., Kornfeld H. Molecular and Functional Analysis of a Lymphocyte Chemoattractant Factor Association of Biologic Function with CD4 Expression. Proc. Natl. Acad. Sci. U.S.A. 91:1994;5109-5113.
17. Moseley A.M. Current Trends in Differential Expression Proteomics Isotopically Coded Tags. Trends Biotech. 19:2001;S10-S16.
18. Percherancier Y., Lagane B., Planchenault T., Staropoli I., Altmeyer R., Virelizier J.-L., Arenzana-Seisdedos F., Hoessli D.C., Bachelerie F. HIV-1 Entry into T-cells Is Not Dependent on CD4 and CCR5 Localization to Sphingolipid-Enriched, Detergent-Resistant Raft Membrane Domains. J. Biol. Chem. 278:2003;3153-3161.
19. Husi H., Grant S.G.N. Isolation of 2000-kDa Complexes of N-Methyl-D-Aspartate Receptor and Postsynaptic Density 95 from Mouse Brain. J. Neurochem. 77:2001;281-291.
20. Tingley W.G., Roche K.W., Thompson A.K., Huganir R.L. Regulation of NMDA Receptor Phosphorylation by Alternative Splicing of the C-Terminal Domain. Nature. 364:1993;70-73.
21. Knuesel, M. P., Wan, Y., Xiao, Z., Holinger, E. P., Lowe, N., Wang, W., Liu, X. Identification of Novel Protein-Portein Interactions Using a Versatile Mammalian TAP Expression System. Mol. Cell. Proteomics, unpublished. [Epub ahead of print]
22. Thuillier L., Hivroz C., Fagard R., Andreaoli C., Mangeat P. Ligation of CD4 Surface Antigen Induces Rapid Tyrosine Phosphorylation of the Cytoskeletal Proten Ezrin. Cell. Immunol. 156:1994;322-331.
23. Kinch M.S., Sanfridson A., Doyle C. The Protein Tyrosine Kinase p56lck Regulates Cell Adhesion Mediated by CD4 and Major Histocompatibility Complex Class II Proteins. J. Exp. Med. 180:1994;1729-1739.