Facile Quantification of Enantiomeric Excess and Concentration with Indicator-Displacement Assays: An Example in the Analyses of α-Hydroxyacids

Journal of the American Chemical Society

Volumn 126, Issue 12, 2004, Pages 3676-3677

  Zhu, Lei ^a   Anslyn, Eric V ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 HYDROXYACID; ATROLACTIC ACID; BORONIC ACID DERIVATIVE; CATECHOL;

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; ASSAY; CATALYST; CHIRALITY; CONCENTRATION (PARAMETERS); ENANTIOMER; ENANTIOMERIC EXCESS; ENANTIOSELECTIVITY; HIGH THROUGHPUT SCREENING; INDICATOR DISPLACEMENT ASSAY; MATHEMATICAL ANALYSIS; QUANTITATIVE ANALYSIS; RECEPTOR BINDING; ULTRAVIOLET SPECTROSCOPY;

CATALYSIS; HYDROXY ACIDS; KINETICS; MODELS, CHEMICAL; MOLECULAR STRUCTURE; STEREOISOMERISM;

EID: 1642404953     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja031839s     Document Type: Article

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34. Judged by the displacement profiles of PL with achiral receptor 1, the absorbance change is most sensitive when [PL]/[1] is between 2 and 6, which accounts for [PL] between 1 and 4 mM in the ensemble. Samples were prepared so that [PL] would fall into the effective range. The method is effective across the entire ee range and is more sensitive when the chiral receptor in use binds stronger with the minor enantiomer in the sample.