Cloning and expression of Lipomyces starkeyi α-amylase in Escherichia coli and determination of some of its properties

FEMS Microbiology Letters

Volumn 233, Issue 1, 2004, Pages 53-64

  Kang, Hee Kyoung ^a   Lee, Jin Ha ^a   Kim, Doman ^a   Day, Donal F ^b   Robyt, John F ^c   Park, Kwan Hwa ^d   Moon, Tae Wha ^d  

^a Chonnam National University   (South Korea)

^b Audubon Sugar Institute   (United States)

^c IOWA STATE UNIVERSITY   (United States)

^d Seoul National University   (South Korea)

Author keywords

amylase; Cloning; Expression; Lipomyces starkeyi

Indexed keywords

ACARBOSE; AMINO ACID; AMYLASE; CALCIUM ION; COMPLEMENTARY DNA; COPPER ION; DEXTRANASE; DNA FRAGMENT; EDETIC ACID; EGTAZIC ACID; ENZYME ANTIBODY; FUNGAL ENZYME; MAGNESIUM ION; MALTODEXTRIN; MUTANT PROTEIN; NUCLEOTIDE; POLYACRYLAMIDE GEL; STARCH;

ACIDITY; ALKALINITY; ANIMAL CELL; ARTICLE; CALCULATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROLYSIS; INTRON; LIPOMYCES STARKEYI; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAINING; TEMPERATURE; WESTERN BLOTTING; YEAST;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, FUNGAL; ECTHYMA, CONTAGIOUS; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, FUNGAL; HYDROGEN-ION CONCENTRATION; INTRONS; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYSACCHARIDES; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SACCHAROMYCETALES; SEQUENCE ANALYSIS, DNA; STARCH; SUBSTRATE SPECIFICITY; TEMPERATURE; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; LIPOMYCES; LIPOMYCES STARKEYI;

EID: 1642384434     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.01.036     Document Type: Article

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