Directed evolution of a dextransucrase for increased constitutive activity and the synthesis of a highly branched dextran

Journal of Molecular Catalysis B: Enzymatic

Volumn 26, Issue 3-6, 2003, Pages 167-176

  Kang, Hee Kyoung ^a   Seo, Eun Seong ^a   Robyt, John F ^b   Kim, Doman ^a  

^a Chonnam National University   (South Korea)

^b IOWA STATE UNIVERSITY   (United States)

Author keywords

Constitutivity; Dextran; Dextransucrase; Directed bacterial evolution; E. coli dextransucrase transformants; Leuconostoc mesenteroides mutants

Indexed keywords

AMINO ACIDS; CARBOHYDRATES; ESCHERICHIA COLI; GENES; NUCLEIC ACIDS; X RAYS;

OPEN READING FRAME (ORF);

ENZYMES;

DEXTRANSUCRASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE DELETION; IMMUNOHISTOCHEMISTRY; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN EXPRESSION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LEUCONOSTOC; LEUCONOSTOC MESENTEROIDES;

EID: 0242659251     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2003.05.006     Document Type: Article

References (45)

1. Robyt J.F. Adv. Carbohydr. Chem. Biochem. 51:1995;133.
2. Jeanes A.W., Haynes A.W., Williams C.A., Rankin J.C., Melvin E.H., Austin M.J., Cluskey J.E., Fisher B.E., Tsuchiya H.M., Rist C.E. J. Am. Chem. Soc. 76:1954;5041.
3. Kim D., Robyt J.F. Enzyme Microb. Technol. 17:1995;689.
4. Kim D., Kim D.W., Lee J.H., Park K.H., Day L.M., Day D.F. Biotechnol. Technol. 11:1997;319-321.
5. Kim D., Robyt J.F. Enzyme Microb. Technol. 16:1995;1010.
6. J.F. Robyt, in: Encyclopedia of Polymer Science and Engineering, fourth ed., Wiley, New York, 1986, p. 752.
7. Kim D., Robyt J.F. Enzyme Microb. Technol. 16:1994;659.
8. Kim D., Kim D.W. J. Microbiol. Biotechnol. 9:1999;219.
9. Seymour F.R., Knapp R.D., Bishop S.H., Jeanes A. Carbohydr. Res. 68:1979;123.
10. Seymour F.R., Chen E.C.M., Bishop S.H. Carbohydr. Res. 68:1979;113.
11. Kim H.S., Kim D., Ryu H.J., Robyt J.F. J. Microbiol. Biotechnol. 10:2000;559.
12. Monchois V., Willemot R.M., Remaud-Simeon M., Croux C., Monsan P. Gene. 182:1996;23.
13. Monchois V., Simeon M.R., Monsan P., Willemot R.M. FEMS Microbiol. Lett. 159:1998;307.
14. Ryu H.J., Kim D., Kim D.W., Moon Y.Y., Robyt J.F. Biotechnol. Lett. 22:2000;421.
15. Banas J.A., Russell R.R.B., Ferretti J.J. Infect. Immun. 58:1990;667.
16. Ferretti J., Gilpin M., Russell R. J. Bacteriol. 169:1987;4271.
17. Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S. FEMS Microbiol. Lett. 161:1998;331.
18. Mooser G., Hefta A., Paxton R., Shively J., Lee T. J. Biol Chem. 266:1991;8916.
19. Park M.R., Ryu H.J., Kim D., Choe J.Y., Robyt J.F. J. Microbiol. Biotechnol. 11:2001;628.
20. E.E. Koch, Handbook on synchrotron radiation, third ed., North-Holland, New York, 1983, p. 103.
21. E.F. Maniatis, E.F. Fritsch, J. Sambrook, Molecular cloning, second ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989, p. 1.25.
22. Sun J., Wanda S., Curtiss R. J. Bacteriol. 177:1995;1703.
23. Bradford M.M. Anal. Biochem. 72:1976;248.
24. Miller A.W., Eklund S.H., Robyt J.F. Carbohydr. Res. 147:1986;119.
25. Mukerjea R., Kim D., Robyt J.F. Carbohydr. Res. 292:1996;11.
26. Laemmli U.K. Nature. 227:1997;680.
27. Miller A.W., Robyt J.F. Anal. Biochem. 156:1986;357.
28. Towbin H., Staehelin T., Gordon J. Proc. Natl. Acad. Sci. 76:1979;4350.
29. Van Cleve J.W., Schaefer W.C., Rist C.E. J. Am. Chem. Soc. 78:1956;4435.
30. Jeanes A., Seymour F.R. Carbohydr. Res. 74:1979;31.
31. Robyt J.F., Mukerjea R. Carbohydr. Res. 251:1994;187.
32. Shimamura A. Carbohydr. Res. 185:1989;239.
33. Shokri A., Sanden A.M., Larsson G. Appl. Microbiol. Biotechnol. 60:2003;654.
34. Cornelis P. Curr. Opin. Biotechnol. 11:2000;450.
35. Yokota N., Kuroda T., Matsuyama S., Tokuda H. J. Biol. Chem. 274:1999;30955.
36. Blight M.A., Chervaux C., Holland I.B. Curr. Opin. Biotechnol. 5:1994;468.
37. Hederson I.R., Navarro-Garcia F., Nataro J.P. Trends Microbiol. 6:1998;370.
38. Kim D., Robyt J.F. Enzyme Microb. Technol. 17:1995;1050.
39. Botchway S.W., Stevens D.L., Hill M.A., Jenner T.J., O'neill P. Radiat. Res. 148:1997;317.
40. Djouzy Z., Andrieux C., Pelenc V., Somarriba S., Popot F., Paul F., Monsan P., Szylit O. J. Appl. Bacteriol. 79:1995;117.
41. Valette P., Pelenc V., Djouzy Z., Andrieux C., Paul F., Monsan P., Szylit O. J. Sci. Food Agric. 62:1993;121.
42. Monsan P., Paul F. FEMS Microbiol. Rev. 16:1995;187.
43. Monchois V., Remaud-Simeon M., Russel R.R.B., Monsan P., Willemot R.M. Appl. Microbiol. Biotechnol. 48:1997;465.
44. Bozonnet S., Dols-Laffargue M., Fabre E., Pizzut S., Remaud-Simeon M., Monsan P., Willemot R.M. J. Bacteriol. 184:2002;5753.
45. Arguello-Morales M., Remaud M., Pizzut S., Sarcabal P., Willemot R., Monsan P. FEMS Microbiol. Lett. 182:2000;81.