Molecular cloning and determination of the nucleotide sequence of a gene encoding an acid-stable α-amylase from Aspergillus kawachii

Journal of Fermentation and Bioengineering

Volumn 81, Issue 4, 1996, Pages 292-298

  Kaneko, Akihiro ^b,c   Sudo, Shigetoshi ^a   Takayasu Sakamoto, Yuko ^a   Tamura, Gakuzo ^b   Ishikawa, Takeaki ^a   Oba, Toshiteru ^a  

^a NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

^b Res Inst of Brewing Rsrc Co Ltd   (Japan)

^c MERCIAN CORPORATION   (Japan)

Author keywords

acid stable amylase gene; Aspergillus kawachii; nucleotide sequence

Indexed keywords

AMYLASE; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ASPERGILLUS; CARBOXY TERMINAL SEQUENCE; EXON; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURAL GENE;

EID: 0029885397     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0922-338X(96)80579-4     Document Type: Article

References (22)

1. Mikami, S., Iwano, K., Shiinoki, S., and Shimada, T.: Purification and some properties of acid-stable α-amylase from shochu-koji (Aspergillus kawachii). Agric. Biol. Chem., 51, 2495-2501 (1987).
2. Sudo, S., Ishikawa, T., Takayasu-Sakamoto, Y., Sato, K., and Oba, T.: Characteristics of acid-stable α-amylase production by submerged culture of Aspergillus kawachii. J. Ferment. Bioeng., 76, 105-110 (1993).
3. Ito, K., Ikemasu, T., and Ishikawa, T.: Cloning and sequencing of the xynA gene xylanase A of Aspergillus kawachii. Biosci. Biotech. Biochem., 56, 906-912 (1992).
4. Shibuya, I., Tamura, G., Ishikawa, T., and Kara, S.: Cloning of the α-amylase cDNA of Aspergillus shirousamii and its expression in Saccharomyces cerevisiae. Biosci. Biotech. Biochem., 56, 174-179 (1992).
5. Iimura, Y., Gomi, K., Uzu, H., and Hara, S.: Transformation of Aspergillus oryzae through plasmid-mediated complementation of the methonine-auxotrophic mutation. Agric. Biol. Chem., 51, 323-328 (1987).
6. Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., and Arnheim, N.: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350-1354 (1985).
7. GeneAmp PCR method Application Protocol No. 1, Takara Shuzo Co. Ltd., Kyoto (1993). (in Japanese)
8. Messing, J.: New M13 vectors for cloning. Methods Enzymol., 101, 20-78 (1983).
9. The annotation of the official method of analysis of the National Tax Administration, 4th ed., p. 213-218. The Brewing Society of Japan, Tokyo (1993). (in Japanese)
10. Dubois, M., Gilles, K. A., Hamiltone, J. K., Reberes, P. A., and Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-356 (1956).
11. Boel, E., Brady, A., Brzozowski, A. M., Derewenda, Z., Dodson, G. G., Jensen, V. J., Petersen, S. B., Swift, H., Thim, L., and Woldike, H. F.: Calcium binding in α-amylases: X-ray diffraction study at 2.1Å resolution of two enzymes from Aspergillus. Biochemistry, 29, 6244-6249 (1990).
12. Toda, H., Kondo, K., and Narita, K.: The complete amino acid sequence of Taka-amylase A. Proc. Jpn. Acad., 58, 200-212 (1982).
13. Tada, S., limura, Y., Gomi, K., Takahashi, K., Hara, S., and Yoshizawa, K.: Cloning and nucleotide sequence of the genomic Taka-amylase A gene of Aspergillus oryzae. Agric. Biol. Chem., 53, 593-599 (1989).
14. Sudo, S., Ishikawa, T., Sato, K., and Oba, T.: Comparison of acid-stable α-amylase production by Aspergillus kawachii in solid-state and submerged cultures. J. Ferment. Bioeng., 77, 483-489 (1994).
15. Gurr, S. J., Unkles, S. E., and Kinghorn, J.R.: The structure and organization of nuclear genes of filamentous fungi, p. 93-139, Gene structure in eukaryotic microbes. IRL Press, Oxford (1987).
16. Hata, Y., Kitamoto, K., Gomi, K., Kumagai, C., and Tamura, G.: Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase. Curr. Genet., 22, 85-91 (1992).
17. Tada, S., Gomi, K., Kitamoto, K., Kumagai, C., Tamura, G., and Hara, S.: Identification of the promoter region of the Taka-amylase A gene required for starch induction. Agric. Biol. Chem., 55, 1939-1941 (1991).
18. Tsuchiya, K., Tada, S., Gomi, K., Kitamoto, K., Kumagai, C., and Tamura, G.: Deletion analysis of the Taka-amylase A gene promoter using a homologous transformation system in Aspergillus oryzae. Biosci. Biotech. Biochem., 56, 1849-1853 (1992).
19. Hayashida, S., Nakahara, K., Kuroda, K., Miyata, T., and Iwanaga, S.: Structure of the raw-starch-afflnity site on the Aspergillus awamori var. kawachi glucoamylase I molecule. Agric. Biol. Chem., 53, 135-141 (1989).
20. Hayashida, S., Nakahara, K., Kanlayakrit, T., Hara, T., and Teramoto, Y.: Characteristics and function of raw-starch-afflnity site on Aspergillus awamori var. kawachi glucoamylase I molecule. Agric. Biol. Chem., 53, 143-149 (1989).
21. Matsuura, Y., Kusunoki, M., Harada, W., Tanaka, N., Iga, Y., Yasuoka, N., Toda, H., Narita, K., and Kakudo, M.: Molecular structure of Taka-amylase A, I. Backbone chain folding at 3Å resolution. J. Biochem., 87, 1555-1558 (1980).
22. Kusunoki, M., Matsuura, Y., Tanaka, N., and Kakudo, M.: X-ray structure and catalytic mechanism of Taka-amylase A. J. Jpn. Soc. Starch Sci., 30, 141-148 (1983). (in Japanese)