Differential mucin expression in colon carcinoma HT-29 clones with variable resistance to 5-fluorouracil and methotrexate

Biology of the Cell

Volumn 96, Issue 2, 2004, Pages 145-151

  Leteurtre, Emmanuelle ^a   Gouyer, Valérie ^a   Rousseau, Karine ^b   Moreau, Odile ^a   Barbat, Alain ^a   Swallow, Dallas ^b   Huet, Guillemette ^a   Lesuffleur, Thécla ^a  

^a INSERM   (France)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Colon cancer cells; Differentiation; Drug resistance; Enterocyte like cells; Mucus secreting cells

Indexed keywords

FLUOROURACIL; METHOTREXATE; MUCIN; MUCIN 1; MUCIN 2; MUCIN 3; MUCIN 4; MUCIN 5AC; MUCIN 5B; MUCIN 6;

ARTICLE; CANCER CELL CULTURE; CARCINOGENESIS; CELL CLONE; CELL MEMBRANE; CELL POLARITY; CELL SUBPOPULATION; COLON CARCINOMA; CONTROLLED STUDY; DRUG SENSITIVITY; EXPERIMENTAL MODEL; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; IN VITRO STUDY; INTESTINE CELL; MONOLAYER CULTURE; MUCUS SECRETION; NORTHERN BLOTTING; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

BLOTTING, NORTHERN; BLOTTING, WESTERN; CLONE CELLS; DRUG RESISTANCE, NEOPLASM; FLUOROURACIL; GENE EXPRESSION REGULATION, NEOPLASTIC; HT29 CELLS; HUMANS; METHOTREXATE; MICROSCOPY, ELECTRON; MUCINS; RNA, MESSENGER;

EID: 1642381066     PISSN: 02484900     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biolcel.2003.12.005     Document Type: Article

References (29)

1. Baldus S.E. Hanisch F.G. Biochemistry and pathological importance of mucin-associated antigens in gastrointestinal neoplasia Adv Cancer Res 79 2000 201-248
2. Bara J. Languille O. Gendron M.C. Daher N. Martin E. Burtin P. Immunohistological study of precancerous mucus modification in human distal colonic polyps Cancer Res 43 1983 3885-3891
3. Biemer-Huttmann A.E. Walsh M.D. McGuckin M.A. Simms L.A. Young J. Leggett B.A. Jass J.R. Mucin core protein expression in colorectal cancers with high levels of microsatellite instability indicates a novel pathway of morphogenesis Clin Cancer Res 6 2000 1909-1916
4. Bresalier R.S. Byrd J.C. Brodt P. Ogata S. Itzkowitz S.H. Yunker C.K. Liver metastasis and adhesion to the sinusoidal endothelium by human colon cancer cells is related to mucin carbohydrate chain length Int J Cancer 76 1998 556-562
5. Buisine M.P. Devisme L. Savidge T.C. Gespach C. Gosselin B. Porchet N. Aubert J.P. Mucin gene expression in human embryonic and fetal intestine Gut 43 1998 519-524
6. Carlstedt I. Herrmann A. Hovenberg H. Lindell G. Nordman H. Wickstrom C. Davies J.R. 'Soluble' and 'insoluble' mucins-identification of distinct populations Biochem Soc Trans 23 1995 845-851
7. Compton C.C. Colorectal carcinoma: diagnostic, prognostic, and molecular features Mod Pathol 16 2003 376-388
8. Devine P.L. McKenzie I.F. Mucins: structure, function, and associations with malignancy Bioessays 14 1992 619-625
9. Hennebicq-reig S. Lesuffleur T. Capon C. De Bolos C. Kim I. Moreau O. Richet C. Hémon B. Recchi M.A. Maës E. Aubert J.P. Real F.X. Zweibaum A. Delannoy P. Degand P. Huet G. Permanent exposure of mucin-secreting HT-29 cells to benzyl-N-acetyl-alpha-D-galactosamide induces abnormal O-glycosylation of mucins and inhibits constitutive and stimulated MUC5AC secretion Biochem J 334 1998 283-295
10. Huet G. Kim I. de Bolos C. Lo-Guidice J.M. Moreau O. Hemon B. Richet C. Delannoy P. Real F.X. Degand P. Characterization of mucins and proteoglycans synthesized by a mucin- secreting HT-29 cell subpopulation J Cell Sci 108 1995 1275-1285
11. Komatsu M. Jepson S. Arango M.E. Carothers Carraway C.A. Carraway K.L. Muc4/sialomucin complex, an intramembrane modulator of ErbB2/HER2/Neu, potentiates primary tumor growth and suppresses apoptosis in a xenotransplanted tumor Oncogene 20 2001 461-470
12. Lesuffleur T. Zweibaum A. Real F.X. Mucins in normal and neoplastic human gastrointestinal tissues Crit Rev Oncol Hematol 17 1994 153-180
13. Lesuffleur T. Barbat A. Dussaulx E. Zweibaum A. Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells Cancer Res 50 1990 6334-6343
14. Lesuffleur T. Violette S. Vasile-Pandrea I. Dussaulx E. Barbat A. Muleris M. Zweibaum A. Resistance to high concentrations of methotrexate and 5-fluorouracil of differentiated HT-29 colon-cancer cells is restricted to cells of enterocytic phenotype Int J Cancer 76 1998 383-392
15. Lesuffleur T. Porchet N. Aubert J.P. Swallow D. Gum J.R. Kim Y.S. Real F.X. Zweibaum A. Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT- 29 cell subpopulations J Cell Sci 106 1993 771-783
16. Lesuffleur T. Roche F. Hill A.S. Lacasa M. Fox M. Swallow D.M. Zweibaum A. Real F.X. Characterization of a mucin cDNA clone isolated from HT-29 mucus- secreting cells. The 3' end of MUC5AC? J Biol Chem 270 1995 13665-13673
17. Lesuffleur T. Barbat A. Luccioni C. Beaumatin J. Clair M. Kornowski A. Dussaulx E. Dutrillaux B. Zweibaum A. Dihydrofolate reductase gene amplification-associated shift of differentiation in methotrexate-adapted HT-29 cells J Cell Biol 115 1991a 1409-1418
18. Lesuffleur T. Kornowski A. Luccioni C. Muleris M. Barbat A. Beaumatin J. Dussaulx E. Dutrillaux B. Zweibaum A. Adaptation to 5-fluorouracil of the heterogeneous human colon tumor cell line HT-29 results in the selection of cells committed to differentiation Int J Cancer 49 1991b 721-730
19. Longley D.B. Harkin D.P. Johnston P.G. 5-fluorouracil: mechanisms of action and clinical strategies Nat Rev Cancer 3 2003 330-338
20. McIlhinney R.A. Patel S. Gore M.E. Monoclonal antibodies recognizing epitopes carried on both glycolipids and glycoproteins of the human milk fat globule membrane Biochem J 227 1985 155-162
21. Olsson N.O. Leclerc A. Jeannin J.F. Martin F. A simple photometric microassay for the quantitative evaluation of macrophage-mediated cytotoxicity on adherent cancer cells Ann Immunol (Paris) 133D 1982 245-254
22. Porchet N. Buisine M.P. Desseyn J.L. Moniaux N. Nollet S. Degand P. Pigny P. Van Seuningen I. Laine A. Aubert J.P. MUC genes: a superfamily of genes? Towards a functional classification of human apomucins J Soc Biol 193 1999 85-99
23. Price M.R. Edwards S. Owainati A. Bullock J.E. Ferry B. Robins R.A. Baldwin R.W. Multiple epitopes on a human breast-carcinoma-associated antigen Int J Cancer 36 1985 567-574
24. Spicer A.P. Rowse G.J. Lidner T.K. Gendler S.J. Delayed mammary tumor progression in Muc-1 null mice J Biol Chem 270 1995 30093-30101
25. Sylvester P.A. Myerscough N. Warren B.F. Carlstedt I. Corfield A.P. Durdey P. Thomas M.G. Differential expression of the chromosome 11 mucin genes in colorectal cancer J Pathol 195 2001 327-335
26. Truant S. Bruyneel E. Gouyer V. De Wever O. Pruvot F.R. Mareel M. Huet G. Requirement of both mucins and proteoglycans in cell-cell dissociation and invasiveness of colon carcinoma HT-29 cells Int J Cancer 104 2003 683-694
27. Van Seuningen I. Pigny P. Perrais M. Porchet N. Aubert J.P. Transcriptional regulation of the 11p15 mucin genes. Towards new biological tools in human therapy, in inflammatory diseases and cancer? Front Biosci 6 2001 D1216-D1234
28. Violette S. Poulain L. Dussaulx E. Pepin D. Faussat A.M. Chambaz J. Lacorte J.M. Staedel C. Lesuffleur T. Resistance of colon cancer cells to long-term 5-fluorouracil exposure is correlated to the relative level of Bcl-2 and Bcl-X(L) in addition to Bax and p53 status Int J Cancer 98 2002 498-504
29. Wickstrom C. Davies J.R. Eriksen G.V. Veerman E.C. Carlstedt I. MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: identification of glycoforms and C-terminal cleavage Biochem J 334 1998 685-693