Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription-PCR-hybridization assay

Journal of Clinical Microbiology

Volumn 37, Issue 9, 1999, Pages 2813-2816

  Blomqvist, S ^a   Skytta A ^a   Roivainen, M ^a   Hovi, T ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CULTURE; GENE ISOLATION; HUMAN; HUMAN RHINOVIRUS; HYBRIDIZATION; INFANT; MAJOR CLINICAL STUDY; PRESCHOOL CHILD; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CHARACTERIZATION; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION;

EID: 0032812763     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.9.2813-2816.1999     Document Type: Article

References (18)

1. Abzug, M. J., A. C. Beam, E. A. Gyorkos, and M. J. Levin. 1990. Viral pneumonia in the first month of life. Pediatr. Infect. Dis. J. 9:881-885.
2. Andréoletti, L., D. Hober, S. Belaich, P. E. Lobert, A. Dewilde, and P. Wattré. 1996. Rapid detection of enterovirus in clinical specimens using PCR and microwell capture hybridization assay. J. Virol. Methods 62:1-10.
3. Arola, M., T. Ziegler, O. Ruuskanen, J. Mertsola, K. Näntö-Salonen, and P. Halonen. 1988. Rhinoviruses in acute otitis media. J. Pediatr. 113:693-695.
4. Arruda, E., and F. G. Hayden. 1993. Detection of human rhinovirus RNA in nasal washings by PCR. Mol. Cell. Probes 7:373-379.
5. Arruda, E., C. E. Crump, B. S. Rollins, A. Ohlin, and F. G. Hayden. 1996. Comparative susceptibility of human embryonic fibroblasts and HeLa cells for isolation of human rhinoviruses. J. Clin. Microbiol. 34:1277-1279.
6. Atmar, R. L., and P. R. Georghiou. 1993. Classification of respiratory tract picornavirus isolates as enteroviruses or rhinoviruses by using reverse transcription-polymerase chain reaction. J. Clin. Microbiol. 31:2544-2546.
7. Couch, R. B. 1992. Rhinoviruses, p. 709-729. In E. H. Lennette (ed.), Laboratory diagnosis of viral infections. Marcel Dekker, Inc., New York, N.Y.
8. Gama, R. E., P. R. Horsnell, P. J. Hughes, C. North, C. B. Bruce, W. Al-Nakib, and G. Stanway. 1989. Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction. J. Med. Virol. 28:73-77.
9. Halonen, P., E. Rocha, J. Hierholzer, B. Holloway, T. Hyypiä, P. Hurskainen. and M. Pallansch. 1995. Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization. J. Clin. Microbiol. 33:648-653.
10. Hyypiä, T., P. Auvinen, and M. Maaronen. 1989. Polymerase chain reaction for human picornaviruses. J. Gen. Virol. 70:3261-3268.
11. Hyypiä, T., T. Puhakka, O. Ruuskanen, M. Mäkelä, A. Arola, and P. Arstila. 1998. Molecular diagnosis of human rhinovirus infections: comparison with virus isolation. J. Clin. Microbiol. 36:2081-2083.
12. Ireland, D. C., J. Kent, and K. G. Nicholsson. 1993. Improved detection of rhinoviruses in nasal and throat swabs by seminested RT-PCR. J. Med. Virol. 40:96-101.
13. Johnston, S. L., G. Sanderson, P. K. Pattemore, S. Smith, P. G. Bardin, C. B. Bruce, P. R. Lambden, D. A. J. Tyrrell, and S. T. Holgate. 1993. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J. Clin. Microbiol. 31:111-117.
14. Johnston, S. L., P. K. Pattemore, G. Sanderson, S. Smith, F. Lampe, L. Josephs, P. Symington, S. O'Toole, S. H. Myint, D. A. J. Tyrrell, and S. T. Holgate. 1995. Community study of role of virus infections in exacerbations of asthma in 9-11 year old children. Br. Med. J. 310:1225-1229.
15. Mori, J., and J. P. Clewley. 1994. Polymerase chain reaction and sequencing for typing rhinovirus RNA. J. Med. Virol. 44:323-329.
16. Nicholson, K. G., J. Kent, and D. C. Ireland. 1993. Respiratory viruses and exacerbations of asthma in adults. Br. Med. J. 307:982-986.
17. Pitkãranta, A., E. Arruda, H. Malmberg, and F. G. Hayden. 1997. Detection of rhinovirus in sinus brushings of patients with acute community-acquired sinusitis by reverse transcription-PCR. J. Clin. Microbiol. 35:1791-1793.
18. Vesanen, M., H. Piiparinen, A. Kallio, and A. Vaheri. 1996. Detection of herpes simplex virus DNA in cerebrospinal fluid samples using the polymerase chain reaction and microplate hybridization. J. Virol. Methods 59:1-11.