Evaluation of Mannheimia haemolytica leukotoxin prepared in nonsupplemented and BSA or FBS supplemented RPMI 1640 medium

Polish Journal of Veterinary Sciences

Volumn 7, Issue 1, 2004, Pages 1-8

  Urban Chmiel, R ^a   Wernicki, A ^a   Puchalski, A ^a   Mikucki, P ^a  

^a UNIVERSITY OF LIFE SCIENCES IN LUBLIN   (Poland)

Author keywords

Leukotoxin; Lkt; Mannheimia haemolytica; Respiratory syndrome

Indexed keywords

BOVINE SERUM ALBUMIN; LEUKOTOXIN; MONOCLONAL ANTIBODY;

ARTICLE; CATTLE; CATTLE DISEASE; CULTURE MEDIUM; EVALUATION; FETAL CALF SERUM; IMMUNOBLOTTING; IMMUNOGENICITY; MANNHEIMIA HAEMOLYTICA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABBIT; SEROTYPE; SUPPLEMENTATION; WESTERN BLOTTING;

ANIMALS; BACTERIAL TOXINS; CATTLE; CULTURE MEDIA; CYTOTOXINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXOTOXINS; MANNHEIMIA HAEMOLYTICA; SERUM ALBUMIN, BOVINE;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; MANNHEIMIA; MANNHEIMIA HAEMOLYTICA; MANNHEIMIA HAEMOLYTICA SEROTYPE 1; ORYCTOLAGUS CUNICULUS;

EID: 1642333504     PISSN: 15051773     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Ambagala TC, Ambagala AP, Srikumaran S (1999) The leukotoxin of Pasteurella haemolytica bind to beta (2) integrins on bovine leukocytes. Microb Lett 179: 161-167.
2. Ball JH, Connolly M, Cassidy J (1993) Pasteurella haemolytica isolated in Northen Ireland during 1989 -1991. Br Vet J 149: 561-570.
3. Baluyut CS, Simonson RR, Bemrick WJ, Maheswaran SK (1981) Interaction of Pasteurella haemolytica with bovine neutrophils: Identification and partial characterization of a cytotoxin. Am J Vet Res 42: 1920-1926.
4. Berggren KA, Baluyut CS, Simonson RR, Bemrick WJ, Maheswaran SK (1981) Cytotoxic effects of P. haemolytica on bovine neutrophils. Am J Vet Res 42: 1383-1388.
5. Burrows LL, Olah - Winfield E, Lo RYC (1993) Molecular analysis of the leukotoxin determinants from P. haemolytica serotypes 1 to 16. Infect Immun 61: 5001-5007.
6. Chang YF, Renshaw HW, Young RY (1988) Pneumonic pasteurellosis: Examination of typable and untypable P. haemolytica strains for leukotoxin production, plasmid content, and antimicrobial susceptibility. Am J Vet Res 48: 378-384.
7. Chang YF, Young R, Post D, Struck DK (1987) Identyfication and characterization of the P. haemolytica leukotoxin. Infect Immun 55: 2348-2354.
8. Chidambaram M, Sharma B, Petras FS, Reese PC, Froshauer S, Weinstock MG (1995) Isolation of P. haemolytica leukotoxin mutans. Infect Immun 63: 1027-1032.
9. Cho HJ, Bohac JG, Yates WDG (1984) Anticytotoxin activity of bovine sera and fluids against P. haemolytica A1 cytotoxin. Can J Comp Med 48: 151-155.
10. Clinkenbeard KD, Clarke CR, Morton RJ, Panciera RJ, Confer AW, Mosier DA (1992) Role of P.haemolytica leukotoxin in virulence and immunity in shipping fever pneumonia. Comp Food Anim Pract 14: 1249-1263.
11. Clinkenbeard KD, Clinkenbeard PA, Waurzyniak BJ (1995) Chaotropic agents cause disaggregation and enhanced activity of Pasteurella haemolytica leukotoxin. Vet Micobiol 45: 201-209.
12. Clinkenbeard KD, Mosier DA, Confer AW (1989) Effect of Pasteurella haemolytica on isolated bovine neutrophils. Toxicon 27: 797-804.
13. Clinkebeard DK, Upton LM (1991) Lysis of bovine plateles by Pasteurella haemolytica leukotoxin. Am J Vet Res 52: 453-457.
14. Confer AW, Clinkenbeard KD, Gatewood DM, Driskel BA, Montelongo M (1997) Serum antibody responses of cattle vaccinated with partially purified native Pasteurella haemolytica leukotoxin. Vaccine 15: 1423-1429.
15. Confer AW, Durham JA (1992) Sequential development of antigens and toxins of Pasteurella haemolytica serotype 1 grown in cell culture medium. Am J Vet Res 53: 646-649.
16. Confer AW, Panciera RJ, Mosier DA (1986) Serum antibodies to Pasteurella haemolytica lipopolysaccharide: Relationship to experimantal bovine pneumonic pasteurellosis. Am J Vet Res 47: 1134-1138.
17. Feist H, Geschwend G, Erler W, Schimmel D (1995) Untersuchungen zu den protein der äußeren membran von Pasteurellen - Mitteilung: Polyacrylamidgelelectrophorese von Proteinen der äußeren membrane möglichkeit der differenzierung von P. haemolytica-Stammen. Berl Munch Tierarztl Wochensc 108: 249-256.
18. Frank GH (1986) The role of Pasteurella haemolytica in the bovine respiratory disease complex. Vet Med 91: 838-846.
19. Frank GH (1988) When Pasteurella haemolytica colonizes the nasal passages of cattle. Vet Med 83: 1060-1064.
20. Gentry MJ, Confer AW, Weinberg ED, Homer JT (1986) Cytotoxin (leukotoxin) production by Pasteurella haemolytica: Requirement for an iron - containing compound. Am J Vet Res 47: 1919-1923.
21. Gonzalez CT, Maheswaran SK (1993) The role of induced virulence factors produced by Pasteurella haemolytica in the pathogenesis of bovine pneumonic pasteurellosis: Review and hypothesis. Br Vet J 149: 183-187.
22. Highlander SK (1997) Growth of Pasteurella haemolytica and production of this leukotoxin in semi - defined media Am J Vet Res 58: 749-754.
23. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
24. Lally ET, Hill RB, Kieba IR, Korostoff J (1999) The interaction between RTX toxins and target cells. Trends in Microb 7: 356-361.
25. Lo RYC, Strathdee SA, Shewen PE (1987) Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1 Infect Immun 55: 1987-1996.
26. Mosier DA, Simons KR, Chengappa MM, Confer AW (1994) Antigenic composition of Pasteurella haemolytica serotype 1 supernatants from supplemented and nonsupplemented media Am J Vet Res 55: 348-352.
27. Petras FS, Chidambaram M, Illyes FE, Froshrauer S, Weinstock MG, Reese P (1995) Antigenic and virulence properties of P. haemolytica leukotoxin mutan. Infect Immun 63: 1033-1038.
28. Saadati M, Gibbs HA, Parton R, Coote JG (1997) Characterisation of the leukotoxin produced by different strains of Pasteurella haemolytica. J Med Microb 46: 276-284.
29. Shewen PE, Wilkie BN (1983) Pasteurella haemolytica cytotoxin: Production by recognized serotypes and neutralization by type - specific rabbit antisera. Am J Vet Res 44: 715-719.
30. Srinand S, Ames TR, Werdin RE, Yoo SH, Maheswaran SK (1996) Evaluation of three experimental subunit vaccines against pneumonic pasteurellosis in cattle. Vaccine 14: 147-154.
31. Sun Y, Clinkenbeard KD (1998) Serum - free culture of P. haemolytica optimized for leukotoxin production. Am J Vet Res 59: 851-855.
32. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA, 76: 4350-4354.
33. Vega MV, Maheswaran SK, Leininger JR (1987) Adaptation of a colorimetric microtitration assay for quantifying Pasteurella haemolytica A1 leukotoxin and antileukotoxin. Am J Vet Res 48: 1559-1564.
34. Wang JF, Kieba JR, Korostoff J, Guo TL, Yamaguchi N, Rozmiarek H, Billings PC, Shenker BJ, Lally ET (1998) Molecular and biochemical mechanisms of Pasteurella haemolytica leukotoxin - induced cell death. Microb Pathog 25: 317-331.
35. Waurzyniak BJ, Clinkenbeard KD, Confer AW, Srikumaran S (1994) Enchancement of Pasteurella haemolytica leukotoxic activity by bovine serum albumin. Am J Vet Res 55: 1267-1274.
36. Wernicki A, Urban-Chmiel R, Puchalski A, Mikucki P (2001) Utilization of leukotoxin as vaccine antigen in immunoprophylaxis of shipping fever in cattle. Pol J Vet Sci 4: 193-198.
37. Whiteley OL, Maheswaran KS, Weiss JD, Ames RT (1990) Immunohistochemical localization of Pasteurella haemolytica A1-derived endotoxin, leukotoxin and capsular polisaccharide in experimental bovine pasteurella pneumonia. Vet Pathol 27: 150-161.
38. Whiteley OL, Maheswaran KS, Weiss JD, Ames RT (1991) Morphological and morphometrical analysis of the acute response of the bovine alveolar wall to Pasteurella haemolytica A1-derived endotoxin and leukotoxin. J Comp Pathol 104: 23-32
39. Yoo HS, Rajagopal BS, Maheswaran SF, Ames TR (1995) Purified Pasteurella haemolytica leukotoxin induces expression of inflammatory cytokines from bovine alveolar macrophages. Microb Pathol 18: 231-252.